Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)

Huang, Jian; Tang, Ding; Shen, Yi; Qin, Baoxiang; Hong, Lilan; You, Aiqing; Li, Ming; Wang, Xin; Yu, Hengxiu; Gu, Minghong; Cheng, Zhukuan

doi:10.1016/s1673-8527(09)60022-9

Cited by 99 publications

(66 citation statements)

References 43 publications

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“…However, it should be noted that GA2ox4 did not respond to application of GA 3 in M82 and pro DGRAS , without prior exposure to PAC, and the PAC treatment itself, typically weakly promoted expression. Similar results were found previously in rice (Huang et al, 2010). …”

Section: Della-independent Ga Responsessupporting

confidence: 92%

Uncovering DELLA-Independent Gibberellin Responses by Characterizing New TomatoproceraMutants

et al. 2015

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Gibberellin (GA) regulates plant development primarily by triggering the degradation/deactivation of the DELLA proteins. However, it remains unclear whether all GA responses are regulated by DELLAs. Tomato (Solanum lycopersicum) has a single DELLA gene named PROCERA (PRO), and its recessive pro allele exhibits constitutive GA activity but retains responsiveness to external GA. In the loss-of-function mutant pro DGRAS , all examined GA developmental responses were considerably enhanced relative to pro and a defect in seed desiccation tolerance was uncovered. As pro, but not pro DGRAS , elongation was promoted by GA treatment, pro may retain residual DELLA activity. In agreement with homeostatic feedback regulation of the GA biosynthetic pathway, we found that GA20oxidase1 expression was suppressed in pro DGRAS and was not affected by exogenous GA 3 . In contrast, expression of GA2oxidase4 was not affected by the elevated GA signaling in pro DGRAS but was strongly induced by exogenous GA 3 . Since a similar response was found in Arabidopsis thaliana plants with impaired activity of all five DELLA genes, we suggest that homeostatic GA responses are regulated by both DELLA-dependent and -independent pathways. Transcriptome analysis of GA-treated pro DGRAS leaves suggests that 5% of all GA-regulated genes in tomato are DELLA independent. INTRODUCTIONThe phytohormone gibberellin (GA) regulates numerous developmental processes throughout the plant life cycle, including seed germination, stem elongation, flowering, and fruit set (Yamaguchi, 2008). The signaling pathway from GA perception to transcriptional activation has been intensively studied over the past two decades and its major components have been identified. The nuclear DELLA proteins, a subgroup of the GRAS transcription factors family, suppress GA signaling (Locascio et al., 2013). GA binding to the soluble GIBBERELLIN INSENSITIVE DWARF1 (GID1) receptor triggers GID1 interaction with the DELLA proteins (Ueguchi-Tanaka et al., 2005;Nakajima et al., 2006;Griffiths et al., 2006), which then stimulates assembly of the DELLA proteins into an SCF E3 ubiquitin ligase complex via the GID2/SLEEPY1 F-box proteins. The SCF complex polyubiquitinates the DELLA proteins, targeting them for destruction by the 26S proteosome (Sasaki et al., 2003;Dill et al., 2004;Griffiths et al., 2006;Harberd et al., 2009;Hauvermale et al., 2012). GA, via GID1, can also reduce DELLA activity through a degradation-independent mechanism (Ariizumi et al., , 2013Ueguchi-Tanaka et al., 2008).Despite the central role of DELLAs in GA signaling, the mechanism underlying this regulation is not fully understood. Several studies have shown that protein-protein interactions play a major role in DELLA function. DELLAs bind to various transcription factors and proteins affecting transcription, including PHYTOCHROME-INTERACTING FACTORs (PIFs), ALCATRAZ, MYC2, JASMONATE-ZIM-DOMAIN PROTEIN9, SCARECROW LIKE3 (SCL3), and TCP transcription factors (de Lucas et al., 2008;Feng et al., 2008;Arnaud et al., 2010;Ga...

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Section: Della-independent Ga Responsessupporting

confidence: 92%

Uncovering DELLA-Independent Gibberellin Responses by Characterizing New TomatoproceraMutants

et al. 2015

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“…7C). The GA2-oxidase gene OsGA2ox6, which controls plant height and tiller number (Lo et al, 2008;Huang et al, 2010), was repressed in OX-Ghd7 HJ19 plants (Fig. 7C).…”

Section: Ghd7 Regulates the Transcriptomes Of Multiple Processesmentioning

confidence: 98%

Grain Number,Plant Height,and Heading Date7Is a Central Regulator of Growth, Development, and Stress Response

et al. 2014

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Grain number, plant height, and heading date7 (Ghd7) has been regarded as an important regulator of heading date and yield potential in rice (Oryza sativa). In this study, we investigated functions of Ghd7 in rice growth, development, and environmental response. As a long-day dependent negative regulator of heading date, the degree of phenotypic effect of Ghd7 on heading date and yield traits is quantitatively related to the transcript level and is also influenced by both environmental conditions and genetic backgrounds. Ghd7 regulates yield traits through modulating panicle branching independent of heading date. Ghd7 also regulates plasticity of tiller branching by mediating the PHYTOCHROME B-TEOSINTE BRANCHED1 pathway. Drought, abscisic acid, jasmonic acid, and high-temperature stress strongly repressed Ghd7 expression, whereas low temperature enhanced Ghd7 expression. Overexpression of Ghd7 increased drought sensitivity, whereas knock-down of Ghd7 enhanced drought tolerance. Gene chip analysis of expression profiles revealed that Ghd7 was involved in the regulation of multiple processes, including flowering time, hormone metabolism, and biotic and abiotic stresses. This study suggests that Ghd7 functions to integrate the dynamic environmental inputs with phase transition, architecture regulation, and stress response to maximize the reproductive success of the rice plant.

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“…The RNAi vector (pCam23A) construction and transformation were performed as previously described (Wang et al, 2009;Huang et al, 2010).…”

Section: Rnai Knockdown Of Crc1mentioning

confidence: 99%

CENTRAL REGION COMPONENT1, a Novel Synaptonemal Complex Component, Is Essential for Meiotic Recombination Initiation in Rice

et al. 2013

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Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)

Cited by 99 publications

References 43 publications

Uncovering DELLA-Independent Gibberellin Responses by Characterizing New TomatoproceraMutants

Uncovering DELLA-Independent Gibberellin Responses by Characterizing New TomatoproceraMutants

Grain Number,Plant Height,and Heading Date7Is a Central Regulator of Growth, Development, and Stress Response

CENTRAL REGION COMPONENT1, a Novel Synaptonemal Complex Component, Is Essential for Meiotic Recombination Initiation in Rice

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