Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes

Lipnik‐Štangelj, Metoda; Carman-Krzan, M

doi:10.1007/s00011-004-1247-3

Cited by 30 publications

(19 citation statements)

References 44 publications

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“…The staining indicated that more than 90 % of the cells in our experimental model were astrocytes, which repeats our previous purity results (29).…”

Section: Preparation Of Astrocyte Culturessupporting

confidence: 74%

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Šarc

Lipnik‐Štangelj²

2009

Archives of Industrial Hygiene and Toxicology

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This study compared the effects of toxicity of ethanol and its fi rst metabolite acetaldehyde in rat astrocytes through cell viability and cell proliferation. The cells were treated with different concentrations of ethanol in the presence or absence of a catalase inhibitor 2-amino-1,2,4 triazole (AMT) or with different concentrations of acetaldehyde. Cell viability was assessed using the trypan blue test. Cell proliferation was assessed after 24 hours and after seven days of exposure to either ethanol or acetaldehyde. We showed that both ethanol and acetaldehyde decreased cell viability in a dose-dependent manner. In proliferation studies, after seven days of exposure to either ethanol or acetaldehyde, we observed a signifi cant dose-dependent decrease in cell number. The protein content study showed biphasic dose-response curves, after 24 hours and seven days of exposure to either ethanol or acetaldehyde. Co-incubation in the presence of AMT signifi cantly reduced the inhibitory effect of ethanol on cell proliferation. We concluded that long-term exposure of astrocytes to ethanol is more toxic than acute exposure. Acetaldehyde is a much more potent toxin than ethanol, and at least a part of ethanol toxicity is due to ethanol's fi rst metabolite acetaldehyde.

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“…The staining indicated that more than 90 % of the cells in our experimental model were astrocytes, which repeats our previous purity results (29).…”

Section: Preparation Of Astrocyte Culturessupporting

confidence: 74%

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Šarc

Lipnik‐Štangelj²

2009

Archives of Industrial Hygiene and Toxicology

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“…DRG cultures were serum-deprived and treated with either 10 M histamine (a stimulant of NGF secretion) or 10 M ATP␥S for 24 h. NGF concentrations in conditioned medium prepared from DRG cultures were measured in triplicate by ELISA (R&D Systems, Minneapolis, MN) as previously described (Lipnik-Stangelj and Carman-Krzan, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…org as supplemental material) and found that the concentration of NGF was Ͻ80 pg/ml after 72 h growth in serum-containing medium. Serum starvation, alone or together with ATP␥S, did not stimulate NGF secretion, whereas histamine, a known stimulator of NGF secretion (Lipnik-Stangelj and Carman-Krzan, 2004), significantly ( p Ͻ 0.01) increased NGF concentrations in DRG cultures (supplemental Fig. 2, available at www.jneurosci.org as supplemental material).…”

Section: P2y 2 Activation In Drg Neurons Inhibits Apoptosis Independementioning

confidence: 99%

Inhibition of Apoptosis by P2Y₂Receptor Activation: Novel Pathways for Neuronal Survival

Arthur

Georgi²,

Akassoglou³

et al. 2006

J. Neurosci.

102

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Cell survival is an essential function in the development and maintenance of the nervous system. We demonstrate here a previously unappreciated role for extracellular nucleotide signaling through the P2Y 2 receptor in the survival of neurons: PC12 (pheochromocytoma 12) cells and dorsal root ganglion neurons are protected from serum starvation-induced apoptosis by ATP, UTP, and ATP␥S, an effect mediated via P2Y 2 receptors, as demonstrated by small interfering RNA and genetic knock-out models. This protection occurs independently of neurophin signaling but requires Src activation of ERK (extracellular signal-regulated kinase) and Akt. Moreover, ATP␥S and NGF act synergistically to enhance neuronal survival through enhanced TrkA signaling. The results, which define a novel mechanism for inhibition of apoptosis, implicate parallel, interacting systems-extracellular nucleotides/P2Y 2 receptors and neurotrophin/TrkA-to sustain neuronal survival.

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“…After the cell cultures became confluent (12-14 days), the cells were treated with 1 mM histamine (Sigma Chemical Co., St. Louis) and 10 ng/ml recombinant rat IL-6 (R&D Systems, Minneapolis) for 24 h. In additional experiments, cells were pre-treated with 1 mM histamine for 24 h, and than incubated in the presence of 10 ng/ml IL-6, for the next 24 h. The concentrations of histamine and IL-6 used in the experiment were chosen from dose-response curves obtained in our previous studies [4,7]. After treatment, culture medium was collected and NGF levels determined.…”

Section: Methodsmentioning

confidence: 99%

Histamine and IL-6 interaction in the stimulation of nerve growth factor secretion from cultured astrocytes

Lipnik‐Štangelj

Carman-Krzan

2005

Inflamm. res.

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Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes

Abstract: Our results indicate that histamine H(1)-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.

Cited by 30 publications

References 44 publications

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Inhibition of Apoptosis by P2Y₂Receptor Activation: Novel Pathways for Neuronal Survival

Histamine and IL-6 interaction in the stimulation of nerve growth factor secretion from cultured astrocytes

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Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes

Abstract: Our results indicate that histamine H(1)-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.

Cited by 30 publications

References 44 publications

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Comparison of Ethanol and Acetaldehyde Toxicity in Rat Astrocytes in Primary Culture

Inhibition of Apoptosis by P2Y2Receptor Activation: Novel Pathways for Neuronal Survival

Histamine and IL-6 interaction in the stimulation of nerve growth factor secretion from cultured astrocytes

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Inhibition of Apoptosis by P2Y₂Receptor Activation: Novel Pathways for Neuronal Survival