Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Abstract

BACKGROUND The objective of this work was to evaluate the activation of Candida antarctica lipase B (CALB) in pressurized CO2 or liquefied petroleum gas (LPG) and, thereafter, to evaluate the synthesis of esters using activated CALB as a biocatalyst. Before activation, CALB was immobilized in polyurethane. For the esterification reactions, mechanical or ultrasonic agitations were studied. The stability of CALB after reuse cycles was also evaluated. RESULTS Before activation, the enzymatic activity of immobilized CALB was 789 U g‐1. After activation in pressurized CO2 and LPG, the activities increased to 2486 U g‐1 and 1413 U g‐1, respectively. Consequently, the residual activities of CALB after activation in pressurized CO2 and LPG were 315% and 179%, respectively. For the best condition of reaction under ultrasonic agitation, oleic acid conversion was 51.2% when using non‐activated CALB. Otherwise, oleic acid conversions were 66.5% and 77.1% when using CALB activated in LPG and CO2, respectively. This biocatalyst maintained more than 80% of its residual activity when reused for up to 13 cycles. CONCLUSION Activation of CALB in pressurized CO2 provided enzymatic activities 1.76 times higher than activation in pressurized LPG did. Immobilized CALB had good performance as a stable biocatalyst for esterification reactions under ultrasonic agitation. © 2017 Society of Chemical Industry

Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane

“…Higher temperatures can reduce the viscosity of liquid media, as those studied in this work, thus reducing mass transfer resistances. Similar results were observed for the synthesis of different esters, such as hexyl levulinate (Badgujar & Bhanage, ) and ethyl oleate (Nyari et al, ). Furthermore, other similar results are reported on the acylation of rutin with lauric acid using CALB and Thermomyces lanuginosus lipases immobilized on different supports as catalysts (Razak & Annuar, ).…”

Efficient synthesis of the key chiral alcohol intermediate of Crizotinib using dual‐enzyme@CaHPO4 hybrid nanoflowers assembled by mimetic biomineralization

“…Compared with free enzyme, immobilized enzyme presents several advantages: first, immobilized enzyme can be easily fixed on the surface of carriers of various shapes such as granular, tubular, and membranous, which is easy to remove and facilitates continuous and repeated use. Moreover, improved tolerance to environment conditions can be observed after immobilization, resulting in enhanced stability and longer working cycle for immobilized enzymes.…”

Sustainable enzymatic technologies in waste animal fat and protein management

“…Adewale et al(2016) remarked that the reaction time was significantly reduced by ultrasound compared with traditional method; 96.8% yield was achieved within 20 min. In addition, the application of supercritical CO2 in enzymatic transesterification of waste animal fats to biodiesel is also a promising method to enhance enzyme activity and reaction rate by increasing the mass transfer rate and further reducing the toxicity of methanol to the enzyme(Lee et al, 2017;Nyari et al, 2018) Nyari et al (2018). demonstrated that the enzymatic activity of immobilized Candida antarctica lipase B increased from 189 U/g to 2486 U/g after activation in pressurized CO2,…”