Activation of IGF-1/IGFBP-3 signaling by berberine improves intestinal mucosal barrier of rats with acute endotoxemia

He, Yan; Yuan, Xiaoming; Guoliang, Zhou; Feng, Aiwen

doi:10.1016/j.fitote.2017.11.012

Cited by 18 publications

(10 citation statements)

References 52 publications

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“…In this study, IGF-1 protein and mRNA expression in the ileum decreased after intraperitoneal injection of endotoxin, as reported previously[44]. Intragastric administration of Bifidobacterium increased IGF-1 protein and mRNA expression, indicating that IGF-1 plays an important role in the recovery of ileal mucosal damage.…”

Section: Discussionsupporting

confidence: 90%

Effects of Bifidobacterium infantis on cytokine-induced neutrophil chemoattractant and insulin-like growth factor-1 in the ileum of rats with endotoxin injury

Wang

Sun

Zheng

et al. 2019

WJG

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BACKGROUND The digestive tract is the maximal immunizing tissue in the body, and mucosal integrity and functional status of the gut is very important to maintain a healthy organism. Severe infection is one of the most common causes of gastrointestinal dysfunction, and the pathogenesis is closely related to endotoxemia and intestinal barrier injury. Bifidobacterium is one of the main probiotics in the human body that is involved in digestion, absorption, metabolism, nutrition, and immunity. Bifidobacterium plays an important role in maintaining the intestinal mucosal barrier integrity. This study investigated the protective mechanism of Bifidobacterium during ileal injury in rats. AIM To investigate the effects of Bifidobacterium on cytokine-induced neutrophil chemoattractant (CINC) and insulin-like growth factor 1 (IGF-1) in the ileum of rats with endotoxin injury. METHODS Preweaning rats were randomly divided into three groups: Control (group C), model (group E) and treatment (group T). Group E was intraperitoneally injected with lipopolysaccharide (LPS) to create an animal model of intestinal injury. Group T was intragastrically administered Bifidobacterium suspension 7 d before LPS. Group C was intraperitoneally injected with normal saline. The rats were killed at 2, 6 or 12 h after LPS or physiological saline injection to collect ileal tissue samples. The expression of ileal CINC mRNA was evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and expression of ileal IGF-1 protein and mRNA was detected by immunohistochemistry and RT-PCR, respectively. RESULTS The ileum of rats in Group C did not express CINC mRNA, ileums from Group E expressed high levels, which was then significantly decreased in Group T ( F = 23.947, P < 0.05). There was no significant difference in CINC mRNA expression at different times ( F = 0.665, P > 0.05). There was a high level of IGF-1 brown granules in ileal crypts and epithelial cells in Group C, sparse staining in Group E, and dark, dense brown staining in Group T. There was a significant difference between Groups C and E and Groups E and T ( P < 0.05). There was no significant difference in IGF-1 protein expression at different times ( F = 1.269, P > 0.05). IGF-1 mRNA expression was significantly different among the three groups ( P < 0.05), though not at different times ( F = 0.086, P > 0.05). CONCLUSION Expression of CINC mRNA increased in the ileum of preweaning rats with endotoxin injury, and exogenous administration of ...

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Section: Discussionsupporting

confidence: 90%

Effects of Bifidobacterium infantis on cytokine-induced neutrophil chemoattractant and insulin-like growth factor-1 in the ileum of rats with endotoxin injury

Wang

Sun

Zheng

et al. 2019

WJG

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“…Indeed, in the intestine the activation of Wnt/beta-catenin pathway helps to maintain GVB function [2,34]. Given that berberine is capable of ameliorating impaired gut mucosal barrier [28,[40][41][42][43], in this study we investigated if berberine might maintain GVB function though the modulation of the Wnt/beta-catenin pathway. The experimental findings revealed serum endotoxin level was very low in sham-operated rats but significantly elevated in CLP matches, indicating the possibility that sepsis disturbed GVB function, thereby resulting in substantial entrance of bacteria and toxins into the bloodstream through capillaries which are located beneath the intestinal epithelium [2].…”

Section: Discussionmentioning

confidence: 99%

“…24 hr later, the rats were perfused transcardially with saline. EB was extracted from the scraped fresh mucosal tissues [28] of distal jejunum by incubation in 5 ml of formamide at 54 °C for 24 hr. EB was quantified by measuring its absorption at a wavelength of 620 nm by a spectrometer.…”

Section: Jejunal Microvascular Permeabilitymentioning

confidence: 99%

Berberine Exerts a Protective Effect on Gut-Vascular Barrier via the Modulation of the Wnt/Beta-Catenin Signaling Pathway During Sepsis

Yuan

Zuo

et al. 2018

Cell Physiol Biochem

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Background/Aims: The gut-vascular barrier (GVB) has recently been depicted to dampen the bacterial invasion of the bloodstream. The intestinal mucosa is a tissue rich in small vessels including capillaries. In this study, the protective effect of berberine on GVB in small bowel mucosa was investigated. Methods: The rat cecal ligation and puncture (CLP) sepsis model was employed to evaluate the effect of berberine on serum endotoxin level and intestinal vascular permeability to Evans blue in vivo. The rat intestinal microvascular endothelial cells (RIMECs) treated by lipopolysaccharide (LPS) were used to assess the effect of berberine on endothelial permeability to FITC-labeled dextran, transendothelial electrical resistance (TEER), and tight junction (TJ) and adherens junction (AJ) expression in vitro. Results: After 24-hr CLP operation the serum endotoxin concentration and gut vascular permeability were significantly increased, while berberine markedly reduced endotoxin level and vascular leakage. In vitro, LPS not only dramatically increased endothelial permeability of RIMECs to FITC-dextran, but also decreased TEER and inhibited claudin-12, beta-catenin and VE-cadherin expression. These effects of LPS were antagonized by berberine. In addition, our in vivo and vitro studies also confirmed that the effect of berberine on GVB could be partially abolished by ICG001. Conclusion: Berberine exerted a protective effect on GVB function in sepsis, which was strictly related to the modulation of the Wnt/beta-catenin signaling pathway.

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“…The interferon-alpha signaling pathway is activated in the human degenerative annulus fibrosis via induction of 3 IFITs and other genes, such as IGFBP3. In contrast, He et al found that activation of IGF-1/IGFBP3 signaling by berberine improves the intestinal mucosal barrier in rats with acute endotoxemia [ 33 ]. Deng et al reported that IGFBP3 is an important MSC homing molecule and reported on the therapeutic potential of hMSC extracellular matrix in bone regeneration [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model

et al. 2018

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BackgroundThe LIM-homeobox transcription factor islet-1 (ISL1) has been proposed as a marker for cardiovascular progenitor cells. This study investigated whether forced expression of ISL1 in human mesenchymal stem cells (hMSCs) improves myocardial infarction (MI) treatment outcomes.MethodsThe lentiviral vector containing the human elongation factor 1α promoter, which drives the expression of ISL1 (EF1α-ISL1), was constructed using the Multisite Gateway System and used to transduce hMSCs. Flow cytometry, immunofluorescence, Western blotting, TUNEL assay, and RNA sequencing were performed to evaluate the function of ISL1-overexpressing hMSCs (ISL1-hMSCs).ResultsThe in vivo results showed that transplantation of ISL1-hMSCs improved cardiac function in a rat model of MI. Left ventricle ejection fraction and fractional shortening were greater in post-MI hearts after 4 weeks of treatment with ISL1-hMSCs compared with control hMSCs or phosphate-buffered saline. We also found that ISL1 overexpression increased angiogenesis and decreased apoptosis and inflammation. The greater potential of ISL1-hMSCs may be attributable to an increased number of surviving cells after transplantation. Conditioned medium from ISL1-hMSCs decreased the apoptotic effect of H2O2 on the cardiomyocyte cell line H9c2. To clarify the molecular basis of this finding, we employed RNA sequencing to compare the apoptotic-related gene expression profiles of control hMSCs and ISL1-hMSCs. The results showed that insulin-like growth factor binding protein 3 (IGFBP3) was the only gene in ISL1-hMSCs with a RPKM value higher than 100 and that the difference fold-change between ISL1-hMSCs and control hMSCs was greater than 3, suggesting that IGFBP3 might play an important role in the anti-apoptosis effect of ISL1-hMSCs through paracrine effects. Furthermore, the expression of IGFBP3 in the conditioned medium from ISL1-hMSCs was almost fourfold greater than that in conditioned medium from control hMSCs. Moreover, the IGFBP3 neutralization antibody reversed the apoptotic effect of ISL1-hMSCs-CM.ConclusionsThese results suggest that overexpression of ISL1 in hMSCs promotes cell survival in a model of MI and enhances their paracrine function to protect cardiomyocytes, which may be mediated through IGFBP3. ISL1 overexpression in hMSCs may represent a novel strategy for enhancing the effectiveness of stem cell therapy after MI.Electronic supplementary materialThe online version of this article (10.1186/s13287-018-0803-7) contains supplementary material, which is available to authorized users.

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Activation of IGF-1/IGFBP-3 signaling by berberine improves intestinal mucosal barrier of rats with acute endotoxemia

Cited by 18 publications

References 52 publications

Effects of Bifidobacterium infantis on cytokine-induced neutrophil chemoattractant and insulin-like growth factor-1 in the ileum of rats with endotoxin injury

Effects of Bifidobacterium infantis on cytokine-induced neutrophil chemoattractant and insulin-like growth factor-1 in the ileum of rats with endotoxin injury

Berberine Exerts a Protective Effect on Gut-Vascular Barrier via the Modulation of the Wnt/Beta-Catenin Signaling Pathway During Sepsis

ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model

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