Activation of Intestinal Mucosal Immunity in Tumor‐bearing Mice by Lactoferrin

Wang, Wenping; Iigo, Masaaki; Sato, Jun; Sekine, Kazunori; Adachi, Isamu; Tsuda, Hiroyuki

doi:10.1111/j.1349-7006.2000.tb00880.x

Cited by 116 publications

(79 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous report indicated that IFN-ª production by CD4 þ splenocytes in mice is increased by oral administration of LF [30]. LF feeding also increased the number of IFN-ª þ cells in the small intestinal mucosa of mice [31]. These findings suggest the possibility that the macrophage-activating component released from the guinea-pig MNC is an IFN-ª-like cytokine.…”

Section: Discussionmentioning

confidence: 48%

Effect of lactoferrin feeding on the host antifungal response in guinea-pigs infected or immunised with Trichophyton mentagrophytes

Wakabayashi

Takakura

Yamauchi

et al. 2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

Earlier studies revealed that oral administration of lactoferrin (LF), a multi-functional milk protein, facilitated curing of dermatophytosis in guinea-pigs and man by an unknown mechanism. The present study aimed to assess the effect of feeding bovine LF on the host antifungal defence systems in guinea-pigs infected or immunised with Trichophyton mentagrophytes, a dermatophytosis-causing fungus. The unbound ironbinding capacity (UIBC) of the plasma of individual animals varied, and plasma with higher UIBC inhibited growth of T. mentagrophytes in vitro. However, LF administration did not enhance plasma UIBC or the anti-T. mentagrophytes activity of plasma in infected or uninfected animals. Phagocytic activity and reactive oxygen (RO) production of blood neutrophil polymorphonuclear leucocytes (PMNLs) were estimated by flow cytometry. LF administration caused no significant effects on phagocytic activity or RO production of neutrophil PMNLs in infected or uninfected animals. The functions of mononuclear cells (MNC) from the spleen were investigated in guinea-pigs immunised with heat-killed T. mentagrophytes conidia. The MNC were cultured with concanavalin A or inactivated T. mentagrophytes. In the bromo-deoxyuridine incorporation assay, the stimulation index was higher for MNC derived from LF-treated animals than for those from control animals. The culture supernates of MNC enhanced the ability of macrophages to kill T. mentagrophytes conidia. Furthermore, stronger augmentation was observed with the culture supernate from LF-treated animals than with that from control animals. In conclusion, LF feeding may potentiate the host antifungal defence systems by modulating MNC function rather than plasma antifungal activity or peripheral blood neutrophil PMNL activity.

show abstract

Section: Discussionmentioning

confidence: 48%

Effect of lactoferrin feeding on the host antifungal response in guinea-pigs infected or immunised with Trichophyton mentagrophytes

Wakabayashi

Takakura

Yamauchi

et al. 2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…A similar effect in spleen from adult mice fed with colostrum has been described 14 . An increase in the NK cell proportion after lactoferrin administration has been found in circulating cells in adult rats 15 and in intestinal cells in mice 16 . Moreover, our data derived from the end of suckling (day 21) suggested that NKR-P1Aþ LPL from WPC supplemented animals exhibited a more mature intestinal phenotype (NKR-P1Aþ CD8-), which corresponds to that found in adult age, than reference animals did.…”

Section: Discussionmentioning

confidence: 99%

Bovine whey protein concentrate supplementation modulates maturation of immune system in suckling rats

et al. 2007

View full text Add to dashboard Cite

During neonatal life, challenges from breast milk and microbial flora promote immune system maturation. Immunonutrition in these stages may become an important way to increase natural defence systems. The aim of this study was to determine the effect of a daily bovine milk whey protein concentrate (WPC) supplement on the intestinal and systemic immune systems in suckling rats. The composition of intraepithelial and lamina propria lymphocytes (IEL and LPL) was analysed by flow cytometry. Systemic and intestinal humoral immune responses were determined by sera Ig levels and Ig-secreting cell quantification by ELISA and ELISPOT, respectively. From birth, suckling Wistar rats were supplemented with WPC or standard infant formula (SIF). The WPC group showed the same proportion of most of the main mucosal cell subsets as the reference animals. However, in the first days of life WPC enhanced the innate immunity by increasing the NK cell proportion in both epithelial and lamina propria (LP) compartments. A rise in intestinal CD8aaþ IEL was also induced by WPC supplementation. A timecourse of sera Ig levels and spontaneous IgA, IgM and IgG production by LPL and mononuclear cells from blood and spleen, in the WPC group, exhibited a similar pattern to those pups fed only by dam's milk. In summary, the present results show the effects of WPC on enhancing mucosal innate immunity during early life.

show abstract

“…Therefore, bLF may be applicable for inhibition of carcinogenesis by other heterocyclic amines with similar activation processes by CYP1A2. However, as administration of 2% bLF alone slightly, but significantly, reduced DEN/ PH-induced liver carcinogenesis (Table II), the observed up-regulation of immune reactions in the intestine with stimulation of cytotoxic T cells and induction of interleukin-18 in mice, 41) may also play a role. Further studies are required to clarify this point.…”

Section: Discussionmentioning

confidence: 99%

Down‐regulation of 2‐Amino‐3,8‐dimethylimidazo[4,5‐f]quinoxaline (MeIQx)‐induced CYP1A2 Expression Is Associated with Bovine Lactoferrin Inhibition of MeIQx‐induced Liver and Colon Carcinogenesis in Rats

Fujita

Ohnishi

Sekine

et al. 2002

Japanese Journal of Cancer Research

Self Cite

View full text Add to dashboard Cite

The inhibitory influence of bovine lactoferrin (bLF) on induction of preneoplastic hepatic glutathione S-transferase placental form-positive (GST-P + ) cell foci and colon aberrant crypt foci (ACF) by diethylnitrosamine (DEN) and 2-amino-3,8-dimethylimidazo[4,5-f ]quinoxaline (MeIQx) was investigated in F344 rats. Rats were initially treated with DEN, then placed on basal diet containing MeIQx (200 ppm) alone, MeIQx plus 2% bLF, or MeIQx plus 0.2% bLF from week 2 to week 8, with partial hepatectomy performed at week 3. Concomitant administration of 2% or 0.2% bLF with MeIQx caused significant dose-dependent decreases in both number and unit area of GST-P + cell foci (2% bLF, P < < < <0.001; 0.2% bLF, P < < < <0.01). Similar results were observed for MeIQx-induced colon ACF in the groups without DEN treatment (2% and 0.2% bLF, P < < < <0.05). To investigate the underlying mechanisms, we analyzed the influence of bLF on levels of cytochrome P4501A2 (CYP1A2), a metabolically activating enzyme of MeIQx in the liver. The results demonstrated that combined administration of 2% bLF significantly reduced levels of MeIQx-induced CYP1A2 mRNA (P < < < <0.05) and protein (P < < < <0.05) to the normal levels, in association with reduced values for MeIQx-DNA adducts (P < < < <0.05), liver GST-P + cell foci and colon ACF. These results suggest that bLF is a chemopreventive agent for DEN alone or DEN plus MeIQx-induced liver, and MeIQx-induced colon carcinogenesis in rats. One possible mechanism is a normalizing down-regulation of CYP1A2 expression by bLF, with consequent reduction of carcinogen activation and adduct formation.

show abstract

Activation of Intestinal Mucosal Immunity in Tumor‐bearing Mice by Lactoferrin

Cited by 116 publications

References 18 publications

Effect of lactoferrin feeding on the host antifungal response in guinea-pigs infected or immunised with Trichophyton mentagrophytes

Effect of lactoferrin feeding on the host antifungal response in guinea-pigs infected or immunised with Trichophyton mentagrophytes

Bovine whey protein concentrate supplementation modulates maturation of immune system in suckling rats

Down‐regulation of 2‐Amino‐3,8‐dimethylimidazo[4,5‐f]quinoxaline (MeIQx)‐induced CYP1A2 Expression Is Associated with Bovine Lactoferrin Inhibition of MeIQx‐induced Liver and Colon Carcinogenesis in Rats

Contact Info

Product

Resources

About