Activation of JAK/STAT signaling pathway in Drosophila melanogaster S2 cell culture

Шапошников, А. А.; Kryndushkin, A. S.; Nikolenko, Yu. V.; Panov, V. V.; Набирочкина, Е. Н.; Лебедева, Л. А.; Shidlovskii, Yulii V.

doi:10.1134/s0026893313030138

Cited by 1 publication

(1 citation statement)

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“…This type of cell‐based analysis allows for systems‐level analysis of signaling pathways, in which variables such as signal strength or duration may be systematically tested to determine the signaling output that would elicit a meaningful biological outcome. Detailed assay methods and relevant reagents for the various signaling methods are available in the following research and review articles (Albert & Bokel, ; J. Li, Housden, & Bray, ; Shaposhnikov et al, ; Vincent, ).…”

Section: Introductionmentioning

confidence: 99%

Generating and working with Drosophila cell cultures: Current challenges and opportunities

Luhur

Klueg

Zelhof

2018

WIREs Developmental Biology

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The use of Drosophila cell cultures has positively impacted both fundamental and biomedical research. The most widely used cell lines: Schneider, Kc, the CNS and imaginal disc lines continue to be the choice for many applications. Drosophila cell lines provide a homogenous source of cells suitable for biochemical experimentations, transcriptomics, functional genomics, and biomedical applications. They are amenable to RNA interference and serve as a platform for high‐throughput screens to identify relevant candidate genes or drugs for any biological process. Currently, CRISPR‐based functional genomics are also being developed for Drosophila cell lines. Even though many uniquely derived cell lines exist, cell genetic techniques such the transgenic UAS‐GAL4‐based RasV12 oncogene expression, CRISPR‐Cas9 editing and recombination mediated cassette exchange are likely to drive the establishment of many more lines from specific tissues, cells, or genotypes. However, the pace of creating new lines is hindered by several factors inherent to working with Drosophila cell cultures: single cell cloning, optimal media formulations and culture conditions capable of supporting lines from novel tissue sources or genotypes. Moreover, even though many Drosophila cell lines are morphologically and transcriptionally distinct it may be necessary to implement a standard for Drosophila cell line authentication, ensuring the identity and purity of each cell line. Altogether, recent advances and a standardized authentication effort should improve the utility of Drosophila cell cultures as a relevant model for fundamental and biomedical research. This article is categorized under: Technologies > Analysis of Cell, Tissue, and Animal Phenotypes

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Section: Introductionmentioning

confidence: 99%