Activation of Latent Transgenes in Arabidopsis Using a Hybrid Transcription Factor

Guyer, Dave; Tuttle, Ann; Rouse, Sabrina; Volrath, Sandra; Johnson, Marie; Potter, Sharon; Görlach, Jörn; Goff, Steve; Crossland, Lyle; Ward, Eric

doi:10.1093/genetics/149.2.633

Cited by 43 publications

(3 citation statements)

References 22 publications

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“…To create the tomato plants ectopically expressing the PD::AtzDof1.3 , two separate constructs; an activator construct containing the phytoene desaturase promoter, and a target construct containing the AtzDof1.3 transcription factor were transformed separately into individual plants. These two lines were crossed and T 1 plants inheriting both constructs were selected [ 84 , 85 ]. The method used to create these lines was described in detail in Powell et al [ 86 ].…”

Section: Methodsmentioning

confidence: 99%

Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use

Luengwilai

Jiménez

et al. 2022

IJMS

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A large collection of transgenic tomato lines, each ectopically expressing a different Arabidopsis thaliana transcription factor, was screened for variants with alterations in leaf starch. Such lines may be affected in carbon partitioning, and in allocation to the sinks. We focused on ‘L4080’, which harbored an A. thaliana zDof (DNA-binding one zinc finger) isoform 1.3 (AtzDof1.3) gene, and which had a 2–4-fold higher starch-to-sucrose ratio in source leaves over the diel (p < 0.05). Our aim was to determine whether there were associated effects on productivity. L4080 plants were altered in nitrogen (N) and carbon (C) metabolism. The N-to-C ratio was higher in six-week-old L4080, and when treated with 1/10 N, L4080 growth was less inhibited compared to the wild-type and this was accompanied by faster root elongation (p < 0.05). The six-week-old L4080 acquired 42% more dry matter at 720 ppm CO2, compared to ambient CO2 (p < 0.05), while the wild-type (WT) remained unchanged. GC-MS-TOF data showed that L4080 source leaves were enriched in amino acids compared to the WT, and at 49 DPA, fruit had 25% greater mass, higher sucrose, and increased yield (25%; p < 0.05) compared to the WT. An Affymetrix cDNA array analysis suggested that only 0.39% of the 9000 cDNAs were altered by 1.5-fold (p < 0.01) in L4080 source leaves. 14C-labeling of fruit disks identified potential differences in 14-DPA fruit metabolism suggesting that post-transcriptional regulation was important. We conclude that AtzDof1.3 and the germplasm derived therefrom, should be investigated for their ‘climate-change adaptive’ potential.

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Section: Methodsmentioning

confidence: 99%

Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use

Luengwilai

Jiménez

et al. 2022

IJMS

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“…特异表达部位包括: 根、叶鞘(分蘖期、抽穗期)、叶片(分蘖期、抽穗期)、幼叶、茎、茎节、叶枕(叶舌、叶耳)、颖壳、雄蕊、雌蕊、胚、胚乳和全生育期组成型表达 [23] [34,35] . 1998 年修饰过的GAL4/UAS系统首次被运用于拟南芥中 [36] . 2003 年带有GAL4/UAS元件的增强子诱捕载体被引入水稻, 建立获得了组织器官特异表达的系列模式系 [22] .…”

Section: 迄今为止已发展了几种不同类型的Gene Trapunclassified

中国水稻功能基因组研究进展

2009

SCI SIN Vitae C

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“…More than 90% of the world's rice is grown and consumed in Asia, where 60% of the world's population lives. Rice accounts for between 35-60% of the caloric intake of three billion Asians (Guyer et al, 1998;Pravin et al, 2011). Over 150 million hectares of rice are planted annually, covering about 10% of the world's arable land.…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF DIFFERENT HORMONAL CONCENTRATIONS ONIN-VITRO REGENERAT-ION OF RICE (Oryza sativaL.)

Khan¹,

Mumtaz²,

Mehmood³

et al. 2022

Pak. J. Biotechnol

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The present study was aimed to evaluate the effect of different hormonal concentrations on the in vitro growth and multiplication of rice seeds. The experiment was performed in the tissue culture laboratory, department of biotechnology, UAJ&K Muzaffarabad, Azad Kashmir. Seeds were collected from the local market. Seeds were surface sterilized with different concentrations of Chlorox i. e. (30, 50 and 50 %) and germination percentage was calculated. The maximum (70 %) germination was recorded for seeds treated with 50% chlorox. Whereas minimum germination (37.5%) was recorded for seeds treated with 70% chlorox. The seeds were cultured on MS medium supplemented with varying concentrations of IAA (0.1, 0.2, 0.3, 0.75 and 2mg/l) and KIN at (0.5, 1, 1.5 mg/l). Maximum stem length (30.5 cm), root number (20), root length (6.6 cm), leaf number (70) and leaf length (7 cm) was recorded for MS medium supplemented with higher concentration of IAA (2mg/l) and KIN (1.5 mg/l). Minimum stem length (6.5 cm), root length (2.5 cm), root number (1) root length (2.5 cm), leaf number (1) and leaf length 1.7cm) was recorded on MS medium supplemented with IAA (0.3 mg/l) and KIN (1.5 mg/l). Results indicates that maximum germination percentage was observed in seeds treated with 50% chlorox and medium which was supplemented with high amount of IAA and KIN (2.0 mg/l, 1.5mg/l) significantly increase regeneration percentage.

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Activation of Latent Transgenes in Arabidopsis Using a Hybrid Transcription Factor

Cited by 43 publications

References 22 publications

Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use

Ectopic Expression of Arabidopsis thaliana zDof1.3 in Tomato (Solanum lycopersicum L.) Is Associated with Improved Greenhouse Productivity and Enhanced Carbon and Nitrogen Use

中国水稻功能基因组研究进展

EFFECT OF DIFFERENT HORMONAL CONCENTRATIONS ONIN-VITRO REGENERAT-ION OF RICE (Oryza sativaL.)

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