1999
DOI: 10.1016/s0014-5793(99)01328-9
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Activation of matrix metalloproteinase‐2 in human breast cancer cells overexpressing cyclooxygenase‐1 or ‐2

Abstract: Human breast cancer cell line Hs578T was stably transfected with cDNA for cyclooxygenase-1 or -2. When the cells overexpressing cyclooxygenase-1 or -2 were stimulated with concanavalin A, the processing of matrix metalloproteinase-2 was observed with the aid of gelatin zymography. This processing was not seen in mock-transfected and original cells which did not express detectable cyclooxygenase activity. Furthermore, Northern blotting showed 8^13 fold induction of membrane-type 1 matrix metalloproteinase which… Show more

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Cited by 67 publications
(34 citation statements)
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“…COX-2 expressing tumour cells are associated with the production of a number of angiogenic growth factors and the synthesis and activation of matrix metalloproteinases favouring tumour invasion and angiogenesis (Tsujii et al, 1997;Tsujii et al, 1998;Takahashi et al, 1999).…”
Section: Relationship Between Angiogenesis and The Immune Responsementioning
confidence: 99%
“…COX-2 expressing tumour cells are associated with the production of a number of angiogenic growth factors and the synthesis and activation of matrix metalloproteinases favouring tumour invasion and angiogenesis (Tsujii et al, 1997;Tsujii et al, 1998;Takahashi et al, 1999).…”
Section: Relationship Between Angiogenesis and The Immune Responsementioning
confidence: 99%
“…42 COX-1 or COX-2 overexpression in a human breast cancer cell line stimulated induced expression of MMP-2 and MT-1 MMP. 43 Finally, tumor-derived PG can also have a paracrine effect in stimulating the production of hepatocyte growth factor by stromal cells which may in turn stimulate uPA activity in tumor cells. 44 …”
Section: Prostaglandins and Cellular Invasivenessmentioning
confidence: 99%
“…It remains to determine whether this suppressive eect of NSAIDs on the loss of proteoglycans is due either to an inhibition of the production of ODFR (Halliwell, 1995), or to a reduction in the synthesis of metalloproteinases (MMPs) and other proteolytic enzymes and/or to a stimulation of the synthesis and secretion of tissue inhibitors of proteolytic enzymes (Poole et al, 1995). Recent studies also suggest that NSAIDs could act either as reversible enzymatic inhibitors (Barracchini et al, 1998) or by inhibiting COXs which mediate the induction of membranetype metalloproteinase-1 (MMP-14), an enzyme able to activate gelatinase A (MMP-2) and collagenase-3 (MMP-13) (Takahashi et al, 1999).…”
mentioning
confidence: 99%