Activation of Mechanosensitive Ion Channels by Ultrasound

Chu, Yu-Yi; Lim, Jormay; Chien, Andy; Chen, Chih‐Cheng; Wang, Jaw‐Lin

doi:10.1016/j.ultrasmedbio.2022.06.008

Cited by 25 publications

(25 citation statements)

References 86 publications

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“…Offline TUS effects are of particular interest because they may reflect long-term potentiation/depression-like neuroplasticity 2 , lasting longer than transient neuronal adaption effects, with the potential to be used to modulate aberrant activity in brain regions or networks for therapeutic applications. It is thought that TUS induces neuromodulation primarily through mechanical interactions of the ultrasound wave as it passes through cells at the target location 3,4 . However, the mechanism by which this translates into excitatory or inhibitory neuromodulation and its effects on large scale human brain connectivity remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Transcranial focused ultrasound-mediated neurochemical and functional connectivity changes in deep cortical regions in humans

Yaakub

White

Roberts

et al. 2023

Preprint

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Low-intensity transcranial ultrasound stimulation (TUS) is an emerging non-invasive technique for focally modulating human brain function. The mechanisms and neurochemical substrates underlying TUS neuromodulation in humans and how these relate to excitation and inhibition are still poorly understood. In 24 healthy controls, we separately stimulated two deep cortical regions and investigated the effects of theta-burst TUS, a protocol shown to increase corticospinal excitability, on the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) and functional connectivity. We show for the first time in humans that theta-burst TUS selectively reduces GABA levels in the posterior cingulate, but not the dorsal anterior cingulate cortex. Functional connectivity increased following TUS in both regions. Our findings suggest that TUS changes overall excitability by reducing GABAergic inhibition, that changes in TUS-mediated neuroplasticity last at least 50 minutes after stimulation, and that these effects may be state-dependent - a mechanism increasingly recognized to influence the brain's response to neuromodulation.

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Section: Introductionmentioning

confidence: 99%

Transcranial focused ultrasound-mediated neurochemical and functional connectivity changes in deep cortical regions in humans

Yaakub

White

Roberts

et al. 2023

Preprint

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“…It has been shown that ultrasounds, including those of frequency approaching 2 MHz, impacted various mechanosensitive ion channels involved in self‐organization of tissues (Chu et al, 2022; Kim et al, 2022; Wu et al, 2017). In addition to the acoustic effects, cells also respond to various environmental mechanical stimuli, such as compression, substrate stiffness (Biswas, 2020) or shear‐flow (Kim et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Acoustic levitation as a tool for cell‐driven self‐organization of human cell spheroids during long‐term 3D culture

Rabiet,

Arakelian,

Jeger‐Madiot

et al. 2024

Biotech & Bioengineering

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Acoustic levitation, which allows contactless manipulation of micro‐objects with ultrasounds, is a promising technique for spheroids formation and culture. This acoustofluidic technique favors cell–cell interactions, away from the walls of the chip, which leads to the spontaneous self‐organization of cells. Using this approach, we generated spheroids of mesenchymal stromal cells, hepatic and endothelial cells, and showed that long‐term culture of cells in acoustic levitation is feasible. We also demonstrated that this self‐organization and its dynamics depended weakly on the acoustic parameters but were strongly dependent on the levitated cell type. Moreover, spheroid organization was modified by actin cytoskeleton inhibitors or calcium‐mediated interaction inhibitors. Our results confirmed that acoustic levitation is a rising technique for fundamental research and biotechnological industrial application in the rapidly growing field of microphysiological systems. It allowed easily obtaining spheroids of specific and predictable shape and size, which could be cultivated over several days, without requiring hydrogels or extracellular matrix.

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“…A frequently employed strategy revolves around the utilization of MS ion channels, necessitating the discovery and subsequent genetic expression of these channels within target cells. [102] Two primary methods are employed in this endeavor: top-down screening and bottom-up channel engineering. The latter approach, though highly effective, demands considerable labor input, [103] while the former relies predominantly on calcium imaging as its readout, which is characterized by limited sensitivity.…”

Section: Challenges and Future Outlookmentioning

confidence: 99%

Sonogenetics for Monitoring and Modulating Biomolecular Function by Ultrasound

Hahmann,

Ishaqat,

Lammers

et al. 2024

Angewandte Chemie

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Ultrasound technology, synergistically harnessed with genetic engineering and chemistry concepts, has started to open the gateway to the remarkable realm of sonogenetics – a pioneering paradigm for remotely orchestrating cellular functions at the molecular level. This fusion not only enables precisely targeted imaging and therapeutic interventions, but also advances our comprehension of mechanobiology to unparalleled depths. Sonogenetic tools harness mechanical force within small tissue volumes while preserving the integrity of the surrounding physiological environment, reaching depths of up to tens of centimeters with high spatiotemporal precision. These capabilities circumvent the inherent physical limitations of alternative in vivo control methods such as optogenetics and magnetogenetics. In this review, we first discuss mechanosensitive ion channels, the most commonly utilized sonogenetic mediators, in both mammalian and non‐mammalian systems. Subsequently, we provide a comprehensive overview of state‐of‐the‐art sonogenetic approaches that leverage thermal or mechanical features of ultrasonic waves. Additionally, we explore strategies centered around the design of mechanochemically reactive macromolecular systems. Furthermore, we delve into the realm of ultrasound imaging of biomolecular function, encompassing the utilization of gas vesicles and acoustic reporter genes. Finally, we shed light on limitations and challenges of sonogenetics and present a perspective on the future of this promising technology.

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Activation of Mechanosensitive Ion Channels by Ultrasound

Cited by 25 publications

References 86 publications

Transcranial focused ultrasound-mediated neurochemical and functional connectivity changes in deep cortical regions in humans

Transcranial focused ultrasound-mediated neurochemical and functional connectivity changes in deep cortical regions in humans

Acoustic levitation as a tool for cell‐driven self‐organization of human cell spheroids during long‐term 3D culture

Sonogenetics for Monitoring and Modulating Biomolecular Function by Ultrasound

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