Activation of mouse ear lobe tissue regeneration by metabolites of earthworm

Ponesakki, Vasanthakumar; Syamala, Sandhya Soman; Paul, Sayan; Arumugaperumal, Arun; Selvakumar, Padmashree; Vijayan, Vijithkumar; Raj, Audre Preena Maria Sundar; Sivasubramaniam, Sudhakar

doi:10.51470/jez.2023.26.1.1191

Cited by 2 publications

(1 citation statement)

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“…On cDNA templates, a quantitative RT-PCR experiment was performed using the SYBR Green PCR master mix (Bio-Rad) as per the manufacturer's protocols (BioRad) using the designed oligonucleotide primers given in table 1 (Schmidt and Delaney, 2010). A 25µl reaction including 2.5µl of 10x PCR buffer with SyBr green, 2.5µl of 10mM dNTPs, 10pmoles/l of each primer, 10.4µl of PCR water, 0.1µl of 5U Taq polymerase (Genei), and 2µl of cDNA was used to amplify the cagA and housekeeping genes (Actin) (Expósito-Rodríguez et al, 2008;Paul et al, 2021b;Paul et al, 2023;Ponesakki et al, 2023). Initial denaturation of cDNA was performed at 94°C for 10 minutes, followed by 35 cycles of amplification at 94°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds.…”

Section: Real-time Pcrmentioning

confidence: 99%

Stable expression ofHelicobacter pylori cagAoncoprotein in brinjal

Mehran,

Barzigar,

Sathish Kumar

et al. 2023

Preprint

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Helicobacter pylori is closely connected to upper gastrointestinal tract diseases including gastric cancer. Transgenic plants are found to be successful in expressing the bacterial antigens, which could elicit an immune response when consumed. The Cytotoxicity-associated immunodominant antigen protein (cagA) of H. pylori is kindred with pathogenicity and cancer risk. We expressed the cagA transgenically in the brinjal. We amplified the cagA gene from H. pylori strain 26695 chromosomal DNA and transformed it into brinjal callus derived from leaf explants using the pBI121 expression vector. The stable expression and accumulation of the recombinant cagA gene were confirmed by using quantitative real-time PCR, western blot analysis and ELISA. The RT-PCR, western blot and ELISA showed stable expression of cagA gene in the transgenic lines B3, B5, B11, B17 and B21. Among them, B11 and B17 samples showed higher expression of the cagA compared to the other samples. Besides, the immunohistochemistry assay showed the abundant expression of cagA protein in the parenchymal regions of the transgenic plants. Out of the 52 plants, a set five plants were found to be positive for cagA expression. Our experimental outcomes can be used further to design the vaccines against H. pylori from the transgenic brinjal plants.

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Section: Real-time Pcrmentioning

confidence: 99%

Stable expression ofHelicobacter pylori cagAoncoprotein in brinjal

Mehran,

Barzigar,

Sathish Kumar

et al. 2023

Preprint

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Functional significance of earthworm clitellum in regulating the various biological aspects of cell survival and regeneration

Selvan Christyraj,

Vaidhyalingham,

Sengupta

et al. 2024

Developmental Dynamics

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Earthworms are a highly abundant species in nature, with nearly 7000 different species being discovered. Despite the similarities in morphology among earthworm species, their regeneration capabilities vary based on the clitellum. The clitellum plays a crucial role in the clitellum‐dependent worms, as it is involved in the processes of regeneration and reproduction in earthworms. The fascinating characteristic of the clitellum, which serves as a hub for stem cells in clitellum‐dependent worms, plays a crucial role in various biological processes that require further exploration. This review focuses on the overall physiological functions and uncovers the lesser‐known roles of the clitellum that have been documented in various research articles. In recent times, numerous studies have been conducted using the earthworm model to explore various areas. In that regard, the clitellum's different roles in regulating and controlling stem cells, the regeneration process, regulation of organogenesis, stress response, aging, autotomy, and various features have been briefly discussed. Ultimately, we emphasized the unique and versatile role of the clitellum in the animal model, making it an ideal choice for studying development, regeneration, stem cells, organogenesis, toxicology, autotomy, and aging response.

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Activation of mouse ear lobe tissue regeneration by metabolites of earthworm

Cited by 2 publications

References 0 publications

Stable expression ofHelicobacter pylori cagAoncoprotein in brinjal

Stable expression ofHelicobacter pylori cagAoncoprotein in brinjal

Functional significance of earthworm clitellum in regulating the various biological aspects of cell survival and regeneration

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