Activation of nucleolar DNA expression in hepatocytes by glucocorticoids and high density lipoproteins

Панин, Л. Е.; Максимов, В. Ф.; Usynin, I. F.; Korostyshevskaya, I. M.

doi:10.1016/s0960-0760(02)00049-3

Cited by 15 publications

(27 citation statements)

References 10 publications

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“…The intensity of a.b. at 1253 cm Ϫ1 (PO 2 bond) increased to a much less extent compared with oligonucleotide CC(GCC) 5 . Its value is comparable to the same band in the mixture of GM5ЈP with THC.…”

Section: Figure 7 Ir Absorption Spectra For 5ј-cc(gcc) 5 -3јmentioning

confidence: 93%

“…Figure 6 shows IR spectra of oligonucleotide CC(GCC) 5 for control samples and after the action of THC with concentration 1.25 ⅐ 10 Ϫ6 M. In the absorption region of nitrous bases, it was revealed that the integral intensity of absorption shifted by 2-3 cm Ϫ1 to the short-wave region (Fig. 6), which relates to the formation of hydrogen bonds between G-C bases in the process of oligonucleotides structuring.…”

Section: Analysis Of Interaction Of Tetrahydrocortisol With Oligonuclmentioning

confidence: 98%

“…Computer search of the database of mammalian nucleotide sequences (GenBank, EMBL DNA library) revealed the presence of a sequence of CC(GCC) [3][4][5] type at the 3Ј-end nontranslated section of the human apoA-I gene. The same sequence, but with a greater number of iterations, was found in the regulatory region of many mammalian and human genes.…”

Section: Synthesis Of Oligonucleotidesmentioning

confidence: 99%

“…The IR spectra of the interaction between GM5ЈP mononucleotide and THC at a concentration of Essentially different results were obtained in the analysis of the interaction between the CC(GCC) 5 oligonucleotide and THC-apoA-I complex. In this case, the IR spectra of CC(GCC) 5 oligonucleotide mixture with THC at the same concentration of components were used as a control.…”

Section: Figure 7 Ir Absorption Spectra For 5ј-cc(gcc) 5 -3јmentioning

confidence: 99%

“…Tetrahydroderivatives of steroid hormones were previously considered the biologically inactive forms [4]. Then it was shown that in the complex with apolipoprotein A-I (apoA-I), they activate the protein biosynthesis in hepatocytes significantly [5]. This complex also forms in Kupffer cells, with high-density lipoprotein (HDL) providing the source of apoA-I.…”

Section: Introductionmentioning

confidence: 99%

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Effect of glucocorticoids and their complexes with apolipoprotein A‐I on the secondary structure of eukaryotic DNA

Панин¹,

Kunitsyn²,

Tusikov³

2004

Int J of Quantum Chemistry

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ABSTRACT:The tetrahydrocortisol-apolipoprotein A-I complex specifically interacts with eukaryotic DNA isolated from rat liver. This interaction is highly cooperative and of a saturating nature. One DNA molecule binds about 54 molecules of the complex. Smallangle X-ray scattering has shown that hydrogen bonds between nitrous bases are destroyed and that single-stranded structures are formed at the interaction of the tetrahydrocortisolapolipoprotein A-I complex with eukaryotic DNA. The most probable site of binding the tetrahydrocortisol-apolipoprotein A-I complex with DNA is the sequence of the CC(GCC) n type entering the structure of many genes, among them the structure of the human apolipoprotein A-I gene. Oligonucleotide of this type has been synthesized. The association constant (K ass ) of its complexation was shown to be 1.66 ⅐ 10 6 M Ϫ1. Substitution of tetrahydrocortisol for cortisol in the complex results in a considerable decrease of K ass . IRspectroscopy study has shown that the interaction of tetrahydrocortisol with oligonucleotide CC(GCC) 3-5 is accompanied by the formation of hydrogen bonds via the CO-NH, PO 2 , and OH groups of desoxycytidinephosphate. The tetrahydrocortisol-apolipoprotein A-I complex alters the DNA secondary structure formed at the interaction with the hormone, causing the structural transition "order 3 tangle." It is assumed that in the GC-pairs of the given DNA sequence, tetrahydrocortisol initiates the rupture of hydrogen bonds, while the hydrophobic interactions between nitrous bases and apoA-I intensify this process.

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Section: Figure 7 Ir Absorption Spectra For 5ј-cc(gcc) 5 -3јmentioning

confidence: 93%

Section: Analysis Of Interaction Of Tetrahydrocortisol With Oligonuclmentioning

confidence: 98%

Section: Synthesis Of Oligonucleotidesmentioning

confidence: 99%

Section: Figure 7 Ir Absorption Spectra For 5ј-cc(gcc) 5 -3јmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of glucocorticoids and their complexes with apolipoprotein A‐I on the secondary structure of eukaryotic DNA

Панин¹,

Kunitsyn²,

Tusikov³

2004

Int J of Quantum Chemistry

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Effect of complexes of apolipoprotein A-I with tetrahydrocortisol and pregnenolone on protein biosynthesis in rat hepatocytes culture

et al. 2007

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Complexes of apolipoprotein A-I with tetrahydrocortisol and pregnenolone exhibit high biological activity and increase the rate of protein biosynthesis in the culture of rat hepatocytes. An important role in this process is played by reduced delta44-3-keto group in the A-ring of steroid hormones. A complex of apolipoprotein A-I and pregnenolone modulated the rate of protein biosynthesis in liver cells. Hence, the observed changes are not organ-specific for this steroid. Our results suggest that this mechanism of regulation play an important role in intracellular regeneration and proliferation.

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Interaction of apolipoproteins A-I and E in the regulation of DNA, RNA, and protein biosynthesis in cultured rat hepatocytes

et al. 2007

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Experiments on hepatocyte culture showed that apolipoprotein A-I-tetrahydrocortisol complex increases the rate of DNA, RNA, and protein biosynthesis measured by radioactive label incorporation. Apolipoprotein E acted as competitor of the apolipoprotein A-I-tetrahydrocortisol complex and abolished biological activity of the latter. We hypothesize that this mechanism of regulation plays an important role in processes of intracellular regeneration and proliferation.

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Activation of nucleolar DNA expression in hepatocytes by glucocorticoids and high density lipoproteins

Cited by 15 publications

References 10 publications

Effect of glucocorticoids and their complexes with apolipoprotein A‐I on the secondary structure of eukaryotic DNA

Effect of glucocorticoids and their complexes with apolipoprotein A‐I on the secondary structure of eukaryotic DNA

Effect of complexes of apolipoprotein A-I with tetrahydrocortisol and pregnenolone on protein biosynthesis in rat hepatocytes culture

Interaction of apolipoproteins A-I and E in the regulation of DNA, RNA, and protein biosynthesis in cultured rat hepatocytes

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