Activation of P2Y1 receptor triggers two calcium signaling pathways in bone marrow erythroblasts

Paredes‐Gamero, Edgar Julian; Craveiro, Rogerio B.; Pesquero, João Bosco; França, Jerônimo Pereira de; Oshiro, Maria E.M.; Ferreira, Alice Teixeira

doi:10.1016/j.ejphar.2006.01.010

Cited by 18 publications

(16 citation statements)

References 36 publications

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“…A high concentration of ATP (1 mM) was required to promote different effects in the hematopoietic cells. 7, 24 However, this concentration did not induce hematopoietic cell death 6, 17 (Supplementary Figure 2). Concentrations lower than 1 mM did not induce significant changes in the percentage of HSCs (Supplementary Figure 3).…”

Section: Resultsmentioning

confidence: 92%

Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines

et al. 2011

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Extracellular nucleotides are emerging as important regulators of inflammation, cell proliferation and differentiation in a variety of tissues, including the hematopoietic system. In this study, the role of ATP was investigated during murine hematopoiesis. ATP was able to reduce the percentage of hematopoietic stem cells (HSCs), common myeloid progenitors and granulocyte–macrophage progenitors (GMPs), whereas differentiation into megakaryocyte–erythroid progenitors was not affected. In addition, in vivo administration of ATP to mice reduced the number of GMPs, but increased the number of Gr-1+Mac-1+ myeloid cells. ATP also induced an increased proliferation rate and reduced Notch expression in HSCs and impaired HSC-mediated bone marrow reconstitution in sublethally irradiated mice. Moreover, the effects elicited by ATP were inhibited by suramin, a P2 receptor antagonist, and BAPTA, an intracellular Ca2+ chelator. We further investigated whether the presence of cytokines might modulate the observed ATP-induced differentiation. Treatment of cells with cytokines (stem cell factor, interleukin-3 and granulocyte–monocyte colony stimulator factor) before ATP stimulation led to reduced ATP-dependent differentiation in long-term bone marrow cultures, thereby restoring the ability of HSCs to reconstitute hematopoiesis. Thus, our data suggest that ATP induces the differentiation of murine HSCs into the myeloid lineage and that this effect can be modulated by cytokines.

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Section: Resultsmentioning

confidence: 92%

Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines

et al. 2011

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“…The presence of P2Y receptors seems to be a characteristic on hematopoietic cells (7,(13)(14)(15). However, the presence of P2X receptors has also been described in macrophages, platelets, and granulocytic cells (14, 16, 17).…”

Section: Gr-1mentioning

confidence: 99%

Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Paredes‐Gamero

Leon

Borojević

et al. 2008

Journal of Biological Chemistry

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“…The P 1 purinergic receptors recognize adenosine while the P 2 receptors recognize ATP and analogues such as UTP [Burnstock, 1978]. ATP activation of purinergic receptors is responsible for a diverse range of cellular responses such as expansion of intercellular Ca 2þ waves in articular chondrocytes [Kono et al, 2006]; triggering of excocytosis by mobilization of acidic Ca 2þ stores in rat pancreatic beta cells; and both 1,4,5-inositol triphosphate dependent and kinase-dependent increase in Ca 2þ concentration in erythroblasts [Paredes-Gamero et al, 2006]. Because activation of purinergic receptors by ATP is closely linked to cytosolic free calcium levels, internal or external factors that affect activation could result in an alteration in the cytosolic free calcium concentration.…”

Section: Introductionmentioning

confidence: 99%

Real‐time measurement of cytosolic free calcium concentration in HL‐60 cells during static magnetic field exposure and activation by ATP

Belton

Commerford

Hall

et al. 2008

Bioelectromagnetics

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Calcium ions are involved in a number of important signal transduction pathways in cells. Cytosolic calcium concentration ([Ca(2+)](c)) can be affected by the activation of Ca(2+) channels through the action of ligands such as ATP. The response of [Ca(2+)](c) to ligands may be affected by external factors like magnetic fields. The purpose of this study was to determine if exposure to a static magnetic field (SMF) for 800 s altered the [Ca(2+)](c) response to ATP in undifferentiated HL-60 cells. We sham exposed or field exposed fura-2 loaded HL-60 cells to a SMF of 1, 10, and 100 mT. Cells were activated with ATP 300 s into the exposure. The level of [Ca(2+)](c) was followed before, during, and after field or sham exposure with a ratiometric fluorescence spectroscopy system. It was found that high concentrations of ATP resulted in greater [Ca(2+)](c) responses, but faster recovery to near basal levels. The application of 1, 10, or 100 mT SMF did not affect the [Ca(2+)](c) response to ATP. Future work could examine the effect of a longer SMF exposure on the [Ca(2+)](c) response to ATP. Longer exposures might provide sufficient time for morphological changes in the plasma membrane to occur.

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Activation of P2Y1 receptor triggers two calcium signaling pathways in bone marrow erythroblasts

Cited by 18 publications

References 36 publications

Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines

Differentiation of hematopoietic stem cell and myeloid populations by ATP is modulated by cytokines

Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Real‐time measurement of cytosolic free calcium concentration in HL‐60 cells during static magnetic field exposure and activation by ATP

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