Activation of peroxisome proliferation–activated receptor‐γ inhibits transforming growth factor‐β1‐induced airway smooth muscle cell proliferation by suppressing Smad–miR‐21 signaling

Liu, Lu; Pan, Yilin; Zhai, Cui; Zhu, Yanting; Rui, Ke; Shi, Wenhua; Wang, Jian; Yan, Xin; Su, Xiaofan; Song, Yang; Gao, Li; Li, Manxiang

doi:10.1002/jcp.26839

Cited by 25 publications

(13 citation statements)

References 65 publications

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“…On the other hand, HGFs cultured on Zn-Ti had the highest proliferation rate and amount among all groups. Since Smad2 and Smad3 were closely related to cell proliferation, the relatively higher expression of them in HGFs on Zn-Ti than other groups, especially Mg&Zn-Ti whose Zn 2+ release was lower than Zn-Ti, indicated that Zn 2+ might be a main reason in regulating HGFs proliferation [ 65 , 66 ]. It was reported that the function and homeostasis of Zn in cells or organisms were mainly controlled by Zn transporters [ 67 , 68 ].…”

Section: Resultsmentioning

confidence: 99%

Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts

Wang

Luo

Zhang

et al. 2021

Bioactive Materials

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Soft tissue sealing around implants acts as a barrier between the alveolar bone and oral environment, protecting implants from the invasion of bacteria or external stimuli. In this work, magnesium (Mg) and zinc (Zn) are introduced into titanium by plasma immersed ion implantation technology, and their effects on the behaviors of human gingival fibroblasts (HGFs) as well as the underlying mechanisms are investigated. Surface characterization confirms Mg and Zn exist on the surface in metallic and oxidized states. Contact angle test suggests that surface wettability of titanium changes after ion implantation and thus influences protein adsorption of surfaces. In vitro studies disclose that HGFs on Mg ion-implanted samples exhibit better adhesion and migration while cells on Zn ion-implanted samples have higher proliferation rate and amounts. The results of immunofluorescence staining and real-time reverse-transcriptase polymerase chain reaction (RT-PCR) suggest that Mg mainly regulates the motility and adhesion of HGFs through activating the MAPK signal pathway whereas Zn influences HGFs proliferation by triggering the TGF-β signal pathway. The synergistic effect of Mg and Zn ions ensure that HGFs cultured on co-implanted samples possessed both high proliferation rate and motility, which are critical to soft tissue sealing of implants.

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Section: Resultsmentioning

confidence: 99%

Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts

Wang

Luo

Zhang

et al. 2021

Bioactive Materials

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“…The importance of PPARγ activation in the resolution of pneumonia has been confirmed by experimental studies [42,67,68]. A decrease in the proliferation of smooth muscle cells of airways in rats upon the activation of PPARγ was observed [69]. Lakshmi et al have shown that the PPARγ deficiency contributes to the development of airway hyperresponsiveness, bronchial remodeling, and the inhibition of MUC5AC activity [70].…”

Section: Ppar-dependent Mechanisms In Asthmamentioning

confidence: 91%

Peroxisome Proliferator-Activated Receptors as a Therapeutic Target in Asthma

et al. 2020

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The complexity of the pathogenetic mechanisms of the development of chronic inflammation in asthma determines its heterogeneity and insufficient treatment effectiveness. Nuclear transcription factors, which include peroxisome proliferatoractivated receptors, that is, PPARs, play an important role in the regulation of initiation and resolution of the inflammatory process. The ability of PPARs to modulate not only lipid homeostasis but also the activity of the inflammatory response makes them an important pathogenetic target in asthma therapy. At present, special attention is focused on natural (polyunsaturated fatty acids (PUFAs), endocannabinoids, and eicosanoids) and synthetic (fibrates, thiazolidinediones) PPAR ligands and the study of signaling mechanisms involved in the implementation of their anti-inflammatory effects in asthma. This review summarizes current views on the structure and function of PPARs, as well as their participation in the pathogenesis of chronic inflammation in asthma. The potential use of PPAR ligands as therapeutic agents for treating asthma is under discussion.

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“…TGF-β1 has been reported to be elevated and involved in airway remodeling or shortening in patients with asthma [19]. A recent study revealed that introduction of TGF-β1 facilitates proliferation of bronchial epithelial cells and smooth muscle cells in asthma [20,21]. Since TGF-β1 opens up the possibility of cell proliferation, we hypothesized TGF-β1 is required for miR-744-mediated progression in severe asthma.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-744 Inhibits Proliferation of Bronchial Epithelial Cells by Regulating Smad3 Pathway via Targeting Transforming Growth Factor-β1 (TGF-β1) in Severe Asthma

Huang

Liang

et al. 2019

Med Sci Monit

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Background Bronchial epithelial cells proliferation plays a pivotal role in airway remodeling in children with severe asthma. MicroRNAs (miRNAs) have gained great attention for many diseases, including asthma. The purpose of this study was to explore the mechanisms that underlie miR-744 modulating bronchial epithelial cells proliferation in severe asthma in children. Material/Methods Bronchial epithelial cells were isolated from bronchial biopsies of normal controls and asthmatic subjects. miR-744 and transforming growth factor-β1 (TGF-β1) expressions were measured by quantitative reverse transcription PCR (qRT-PCR). Proliferating cell nuclear antigen (PCNA), phosphorylation or total of mothers against decapentaplegic homolog3 (Smad3) and production of Smad anchor for receptor activation (SARA) were measured via Western blot analysis. A link between miR-744 and TGF-β1 was probed by luciferase activity and RNA immunoprecipitation. Cell proliferation was evaluated using the Cell Proliferation Assay Kit. Results Severe asthma showed a significantly elevated cell proliferation rate and reduced abundance of miR-744, which in turn inhibited cell proliferation of bronchial epithelial cells. In particular, TGF-β1 might be a candidate target of miR-744, and enrichment of miR-744 lowered the expression of TGF-β1 at mRNA and protein levels. Indeed, overexpression of miR-744 lowered the proliferation rate of bronchial epithelial cells via driving TGF-β1. Moreover, addition of miR-744 limited phosphorylation of Smad3 but reversed SARA protein abundance by regulating TGF-β1. Conclusions The presence of miR-744 repressed bronchial epithelial cells proliferation through mediating the Smad3 pathway by modulating TGF-β1, providing a promising therapeutic approach for epithelial function in severe asthma.

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Activation of peroxisome proliferation–activated receptor‐γ inhibits transforming growth factor‐β1‐induced airway smooth muscle cell proliferation by suppressing Smad–miR‐21 signaling

Cited by 25 publications

References 65 publications

Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts

Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts

Peroxisome Proliferator-Activated Receptors as a Therapeutic Target in Asthma

MicroRNA-744 Inhibits Proliferation of Bronchial Epithelial Cells by Regulating Smad3 Pathway via Targeting Transforming Growth Factor-β1 (TGF-β1) in Severe Asthma

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