Activation of phospholipase C in SH‐SY5Y neuroblastoma cells by potassium‐induced calcium entry

Abstract

1 We used SH-SY5Y human neuroblastoma cells to investigate whether depolarization with high K+ could stimulate inositol (1,4,5)trisphosphate (Ins(1,4,5)P3) formation and, if so, the mechanism involved.

Influence of Muscarinic Agonists and Tyrosine Kinase Inhibitors on L-type Ca2+Channels in Human and Bovine Trabecular Meshwork Cells

“…The current was dihydropyridine-sensitive : nifedipine reduced, BayK8644 increased inward current. These are characteristics typical of L-type Ca# + channels (McDonald et al, 1994 ;Perez-Reyes and Schneider, 1995 (Misler et al, 1992 ;Smart, Wandless and Lambert, 1995). Thus, Ca# + influx through L-type Ca# + channels could increase [Ca# + ] i in TM cells.…”

The activation of nitric oxide synthase by copper ion is mediated by intracellular Ca2+ mobilization in human pulmonary arterial endothelial cells

“…Additionally, a dihydropyridine sensitive L‐type calcium channel antagonist, nifedipine or nitrendipine, dose‐dependently suppressed the [Ca 2+ ] i increase induced by Cu 2+ , suggesting that the Cu 2+ ‐induced increase in [Ca 2+ ] i is largely due to Ca 2+ entry through an L‐type voltage‐sensitive calcium channel in HPAEC (Carlson et al , 1993; Smart et al , 1995). Although there is very little evidence for voltage‐opened calcium channels in endothelial cells, our study using Ca 2+ ‐agonist, and antagonists, implies the existence of such channels in endothelial cells.…”

Mu-Opioid Receptor-Mediated Phosphorylation of IκB Kinase in Human Neuroblastoma SH-SY5Y Cells

“…Theopioid receptor has been shown to associate with calmodulin directly [14] . The binding of agonist to the -opioid receptor leads to an elevation in cytosolic Ca 2+ [26] and the subsequent activation of calmodulin [14] and its downstream effectors including CaMK-II. Thus, this feature of the -opioid receptor provides an alternative route to activate IKK ␣ ␣ ␤ , independent of PLC ␤ and PKC.…”