Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell

Kim, Myung‐Joo; Choi, Myung-Un; Kim, Chang-Whe

doi:10.1016/j.biomaterials.2006.06.023

Cited by 59 publications

(48 citation statements)

References 46 publications

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“…Several studies have demonstrated the influence of surface roughness on ALP activity [12,22,35]. We could not, however, corroborate the finding of ALP activity increasing with the roughness parameters, as reported by Kim et al [16], though this discrepancy may be attributed to the short duration (21 days) of our experiments.…”

Section: Discussioncontrasting

confidence: 99%

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Lim

Kwon

Sun

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Microarc oxidation (MAO) is a surface treatment that provides nanoporous pits, and thick oxide layers, and incorporates calcium and phosphorus into the coating layer of titanium alloy. We presumed such modification on the surface of titanium alloy by MAO would improve the ability of cementless stems to osseointegrate. We therefore compared the in vitro ability of cells to adhere to MAOed titanium alloy to that of two different types of surface modifications: machined and grit-blasted. We performed energy-dispersive x-ray spectroscopy and scanned electron microscopy investigations to assess the structure and morphology of the surfaces. Biologic and morphologic responses to osteoblast cell lines (SaOS-2) were then examined by measuring cell proliferation, cell differentiation (alkaline phosphatase activity), and avb3 integrin. The cell proliferation rate, alkaline phosphatase activity, and cell adhesion in the MAO group increased in comparison to those in the machined and grit-blasted groups. The osteoblast cell lines of the MAO group were also homogeneously spread on the surface, strongly adhered, and well differentiated when compared to the other groups. This method could be a reasonable option for treating the surfaces of titanium alloy for better osseointegration.

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Section: Discussioncontrasting

confidence: 99%

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Lim

Kwon

Sun

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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“…Previous reports have demonstrated the influence of surface roughness on ALP activity 37,39 . Like a report of Kim et al 36 , ALP activity was enhanced in parallel with the roughness parameters in our study. Moreover this osteoblastic phenotypic characterization can be based on the analysis of various osteobalstic markers including COL1, OSN, OSX and Runx-2 (cbfa-1/OSF-2).…”

Section: A B C D E Fsupporting

confidence: 90%

“…surfaces while their osteoblastic differentiation was enhanced by rough topography [35][36][37][38][39] . Little is known about the regulatory mechanism of the osteoblastic cell adhesion, proliferation, and differentiation on the titanium discs.…”

Section: A B C D E Fmentioning

confidence: 99%

Effect of implant surface microtopography by hydroxyapatite grit-blasting on adhesion, proliferation, and differentiation of osteoblast-like cell line, MG-63

Park

Bae

Kim

et al. 2011

J Korean Assoc Oral Maxillofac Surg

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Objective: This study examined the potential of the in vitro osteogenesis of microtopographically modified surfaces, RBM (resorbable blasting media) surfaces, which generate hydroxyapatite grit-blasting. Methods: RBM surfaces were modified hydroxyapatite grit-blasting to produce microtopographically modified surfaces and the surface morphology, roughness or elements were examined. To investigate the potential of the in vitro osteogenesis, the osteoblastic cell adhesion, proliferation, and differentiation were examined using the human osteoblast-like cell line, MG-63 cells. Osteoblastic cell proliferation was examined as a function of time. In addition, osteoblastic cell differentiation was verified using four different methods of an ALP activity assay, a mineralization assay using alizarin red-s staining, and gene expression of osteoblastic differentiation marker using RT-PCR or ELISA. Results: Osteoblastic cell adhesion, proliferation and ALP activity was elevated on the RBM surfaces compared to the machined group. The cells exhibited a high level of gene expression of the osteoblastic differentiation makers (osteonectin, type I collagen, Runx-2, osterix). imilar data was represented in the ELISA produced similar results in that the RBM surface increased the level of osteocalcin, osteopontin, TGF-beta1 and PGE2 secretion, which was known to stimulate the osteogenesis. Moreover, alizarin red-s staining revealed significantly more mineralized nodules on the RBM surfaces than the machined discs. Conclusion: RBM surfaces modified with hydroxyapatite grit-blasting stimulate the in vitro osteogenesis of MG-63 cells and may accelerate bone formation and increase bone-implant contact.Key words: Osseointegration, Osteogenesis, Microtopography, Surface roughness, RBM (resorbable blasting media)[paper submitted 2011. 2. 8 / revised 2011. 5. 31 / accepted 2011. 6. 7] Abstract (J Korean Assoc Oral Maxillofac Surg 2011;37:214-24)

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“…1C), which is higher than the molecular dimensions of large proteins. However, the Ra value of culture-grade polystyrene plate is approximately 0.05 μm [35], which is higher than that of the titanium surface used in the present study (approximately 0.025 μm). This indicates that the mirror-polished titanium surfaces used in the present study could be regarded as very smooth surface, with no significant topographical features.…”

Section: Discussioncontrasting

confidence: 65%

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

et al. 2010

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Ultraviolet (UV)-photofunctionalization of titanium substantially enhances the strength and quality of osseointegration by promoting osteogenic cellular attachment and proliferation. However, the mechanism underlying the initial interaction between the cells and the surface of the material remains to be elucidated, especially where the influence of surface roughness is excluded as a factor.We evaluated the effect of UV-photofunctionalization on the adhesive strength and cellular stiffness of a single osteoblast and its association with extent of cell spread, cytoskeletal development and focal adhesion assembly on a very smooth titanium surface. Rat bone marrow-derived osteoblasts were cultured on UV-treated or -untreated mirror-polished titanium disks. The mean critical shear force required to initiate detachment of a single osteoblast (n = 10) was over 2000 nN on a UV-treated surface at 3 hr incubation, which was 17 times greater than that on an untreated surface.The mean total energy required to complete the detachment of osteoblasts (n = 10) was consistently over 60 pJ on a UV-treated titanium surface after 24 hr culture, which was up to 42 times greater than that on an untreated surface. Cellular shear modulus, which represents cellular stiffness, was consistently greater on a UV-treated surface than on an untreated surface after 24 hr incubation (n = 10). This strengthening of cell adhesion and cellular mechanical properties on UV-treated titanium was accompanied by enhanced cell spread and actin fiber development and increased levels of vinculin expression. These results indicate that UV-photofunctionalization substantially strengthens osteoblast retention on titanium bulk material with no topographical features and that this is associated with enhancement of intracellular structural development during the cell adhesion process.

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Activation of phospholipase D1 by surface roughness of titanium in MG63 osteoblast-like cell

Cited by 59 publications

References 46 publications

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Effect of implant surface microtopography by hydroxyapatite grit-blasting on adhesion, proliferation, and differentiation of osteoblast-like cell line, MG-63

Enhancement of adhesion strength and cellular stiffness of osteoblasts on mirror-polished titanium surface by UV-photofunctionalization

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