Activation of polyphosphoinositide metabolism at artificial maturation of Patella vulgata oocytes

Borg, Béatrice; Renzis, Guy De; Payan, P.; Ciapa, Brigitte

doi:10.1016/0012-1606(92)90277-n

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…Fertilization (the normal trigger for GVBD in Spisula) initiates a decrease in 32~-labeled phosphatidylinositol bisphosphate (PIPZ), the immediate precursor of IP3, which then recovers after GVBD has occurred. In contrast, in the gastropod Patella, an increase in labeling of PIP2 and no change in IP3 occurred when oocyte maturation was triggered by pH change (Borg, et al, 1992). In some organisms (e.g., in starf~sh;Witchell and Steinhardt, 1990), an increase in [cazfIi appears to be neither necessary nor sufficient to cause GVBD; however, the mechanisms regulating GVBD in deuterostomes (e.g., echinoderms) may differ in this aspect from those of protostomes, such as bivalves.…”

Section: Matsutani and Nomuramentioning

confidence: 66%

Serotonergic mechanisms mediating spawning and oocyte maturation in the zebra mussel,Dreissena polymorpha

Ram¹,

Fong

Kyozuka

1996

Invertebrate Reproduction & Development

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The zebra mussel, Dreissena polymorpha, is a freshwater biofouling bivalve unintentionally introduced in the 1980s into North America from Europe. Oocyte maturation (germinal vesicle breakdown, GVBD) and spawning of the zebra mussel can be triggered with serotonin (5-hydroxytryptamine, 5-HT). In pharmacological experiments to characterize the receptor mediating spawning, the serotonin receptor agonists 8-OH-DPAT, TFMPP, and 1-(1 -naphthyl)piperazine were effective at stimulating spawning; whereas, 2-methylserotonin and alpha-methylserotonin had no effect. In experiments with antagonists of serotonin receptors ketanserin and propranolol had no effect; mianserin, NAN-190, and cyproheptadine had partial inhibitory effects; and methiothepin was a very effective antagonist. Metergoline had mixed agonistlantagonist properties. Ergotamine was the most effective activator of spawning in females. Compared to serotonergic receptors in other organisms, the receptors that activate spawning in zebra mussels resemble SHTlym, 5HTdro2 and human SHTIDP, which are receptors that may act both by inhibiting adenylyl cyclase and by activating phospholipase C. In zebra mussels, 5-HT and 8-OH-DPAT activate GVBD in gonad lkgments, a process also initiated by manual dissection of gonad hgments. GVBD can be inhibited by pre-treatment of ovaries with forskolin and theophylline, suggesting an inhibitory role for cyclic AMP. The ca2+ ionophore A23187 can trigger GVBD and polar body formation. Thus, oocyte maturation in zebra mussels may be initiated via serotonergic receptors simultaneously inhibiting adenylyl cyclase and activating ca2+ mechanisms.

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Section: Matsutani and Nomuramentioning

confidence: 66%

Serotonergic mechanisms mediating spawning and oocyte maturation in the zebra mussel,Dreissena polymorpha

Ram¹,

Fong

Kyozuka

1996

Invertebrate Reproduction & Development

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“…An Ins(1,4,5)P3-induced Ca 2+ release mechanism develops as oocyte maturation advances in starfish (Chiba et al, 1990) and hamster oocytes (Fujiwara et al, 1993). Intracellular stocks of polyphosphoinositides, precursors of Ins(1,4,5)P3, increase between PI and MI in MItype limpet oocytes (Borg et al, 1992). Such circumstances observed in the non-PI-type animals, which all indicate the incomplete establishment of an Ins(1,4,5)P3-mediated Ca 2+ release mechanism at PI stage, may be responsible in part for the inability of Mactra oocytes to use internally stored Ca 2+ at fertilization.…”

Section: Discussionmentioning

confidence: 99%

External Ca2+ is predominantly used for cytoplasmic and nuclear Ca2+ increases in fertilized oocytes of the marine bivalveMactra chinensis

Deguchi

Morisawa

2003

Journal of Cell Science

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IntroductionFully-grown oocytes arrested at the first meiotic prophase (prophase I, PI) in ovaries progress oocyte maturation, when exposed to hormones or released from inhibitory substances, to acquire the ability for fertilization in most animal species. These oocytes are again arrested at species-specific stages including the first metaphase (metaphase I, MI), second metaphase (metaphase II, MII), and pronuclear stage (PN), until fertilization (Masui, 1985). In the bivalves such as Mytilus and Ruditapes, the second arrest of meiosis occurs at MI prior to fertilization (MI-type) (Masui, 1985;Osanai and Kuraishi, 1988). In contrast, there are some bivalve species (e.g. Spisula and Mactra) in which meiosis reinitiation from PI is physiologically triggered concomitantly with fertilization without a process of oocyte maturation (PI-type) (Masui, 1985;Deguchi and Osanai, 1994b). Regardless of the stages of fertilization, single or multiple increases in intracellular Ca 2+ in fertilized oocytes or eggs have been detected in all animal species investigated so far (reviewed by Jaffe, 1985;Miyazaki et al., 1993;Stricker, 1999). The Ca 2+ increases are recognized as essential for the oocytes or eggs to be released from the cell cycle arrest (for a review, see Whitaker and Patel, 1990).As for MI-type bivalves, temporal patterns of Ca 2+ increases at fertilization have been analyzed in five different species: Mytilus, Crassostrea, Ruditapes, Limaria and Hiatella. When the Ca 2+ indicator Fluo-3 is introduced as AM ester, only a single blunt Ca 2+ increase, which persists for several minutes, is observed in fertilized oocytes of Mytilus (Abdelmajid et al., 1993) and Ruditapes (Leclerc et al., 2000). In the oocytes injected with Ca 2+ indicators such as Fura-2 and Calcium Green-1, however, Ca 2+ response at fertilization comprises an initial sharp Ca 2+ transient and subsequent repetitive Ca 2+ spikes (Ca 2+ oscillations) in all of the five species, including Mytilus and Ruditapes (Deguchi and Osanai, 1994a;Deguchi and Morisawa, 1997). In Mytilus, the initial Ca 2+ transient at fertilization arises almost synchronously in the oocyte without forming a point-source Ca 2+ wave (Deguchi and Osanai, 1994a). A more recent analysis revealed that the increased Ca 2+ 367Oocytes of the marine bivalve Mactra chinensis are spawned and arrested at the germinal vesicle stage (first meiotic prophase) until fertilization, without undergoing a process called oocyte maturation. As is the case of other animals, a fertilized oocyte of the bivalve displays increases in intracellular free Ca 2+ . We have clarified here the spatiotemporal patterns and sources of the intracellular Ca 2+ changes at fertilization. Shortly after insemination, increased Ca 2+ simultaneously appeared at the whole cortical region of the oocyte and spread inwardly to the center, attaining the maximal Ca 2+ levels throughout the oocyte, including the cytoplasm and nucleus. The initial maximal Ca 2+ peak was followed by a submaximal plateau phase of cytoplasmic and nuclear...

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Spawning and multiple end points of the embryo-larval bioassay of the blue mussel Mytilus galloprovincialis (Lmk)

Resgalla

2016

Ecotoxicology

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Since the 1960s, little has been done to improve and simulate the use of short-duration chronic bioassays of bivalve embryos, particularly in mussels. However, these test organisms offer great advantages in relation to other groups, due to the ease of obtaining breeders in cultivation systems, in the environment and any time, and due to their high sensitivity to chemicals or contaminants. To contribute some methodological aspects, this study uses techniques to stimulate spawning or improve the obtaining of gametes for use in bioassays with the mussel Mytilus galloprovincialis. It also evaluates different criteria for determining the effect on the larvae, for estimation of EC and NOEC values, based on morphological analysis of developmental delay and the biometrics of the larvae. KCl proved to be a reliable inducer of spawning, with positive responses in 10 of the 12 months of the year tested. Moreover, this chemical, in association with NHCl, demonstrated the capacity to activate immature oocytes obtained from extirpated gonads, enabling an improvement in fertilization rates. The different criteria adopted to determine the effects on the larvae in the assays with reference toxicants (SDS and KCrO) resulted in EC and NOEC values without significant differences, indicating reliability in the results and freedom in the choice of criteria of effect to be adopted in the trials.

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Activation of polyphosphoinositide metabolism at artificial maturation of Patella vulgata oocytes

Cited by 7 publications

References 26 publications

Serotonergic mechanisms mediating spawning and oocyte maturation in the zebra mussel,Dreissena polymorpha

Serotonergic mechanisms mediating spawning and oocyte maturation in the zebra mussel,Dreissena polymorpha

External Ca2+ is predominantly used for cytoplasmic and nuclear Ca2+ increases in fertilized oocytes of the marine bivalveMactra chinensis

Spawning and multiple end points of the embryo-larval bioassay of the blue mussel Mytilus galloprovincialis (Lmk)

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