Activation of skeletal muscle FAPs by LPA requires the Hippo signaling via the FAK pathway

Cruz-Soca, Meilyn; Faundez-Contreras, Jennifer; Córdova-Casanova, Adriana; Gallardo, Felipe; Bock-Pereda, Alexia; Chun, Jerold; Casar, Juan Carlos; Brandan, Enrique

doi:10.1016/j.matbio.2023.03.010

Cited by 6 publications

(1 citation statement)

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“…When partnered with drugs or bioactive molecules, it can improve and accelerate bone formation in vivo . LPA could promote the expression of connective tissue growth factor (CTGF/CCN2), which subsequently promoted the proliferation of MC cells via the LPA 1 receptor to regulate FAP activation . In the study, it was confirmed that the PP-pDA/nHA-LPA scaffold significantly promoted osteoblast proliferation and demonstrated high cell affinity.…”

Section: Resultsmentioning

confidence: 99%

Lysophosphatidic Acid/Polydopamine-Modified nHA Composite Scaffolds for Enhanced Osteogenesis via Upregulating the Wnt/Beta-Catenin Pathway

Chen,

Qian,

et al. 2024

ACS Appl. Mater. Interfaces

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Guided bone regeneration (GBR) technology has been widely used for the regeneration of periodontal bone defects. However, the limited mechanical properties and bone regeneration potential of the currently available GBR membranes often limit their repair effectiveness. In this paper, serum-derived growth factor lysophosphatidic acid (LPA) nanoparticles and dopamine-decorative nanohydroxyapatite (pDA/nHA) particles were double-loaded into polylactic-glycolic acid/polycaprolactone (PLGA/PCL) scaffolds as an organic/inorganic biphase delivery system, namely, PP-pDA/nHA-LPA scaffolds. Physicochemical properties and osteogenic ability in vitro and in vivo were performed. Scanning electron microscopy and mechanical tests showed that the PP-pDA/nHA-LPA scaffolds had a 3D bionic scaffold structure with improved mechanical properties. In vitro cell experiments demonstrated that the PP-pDA/nHA-LPA scaffolds could significantly enhance the attachment, proliferation, osteogenic differentiation, and mineralization of MC3T3-E1 cells. In vivo, the PP-pDA/nHA-LPA scaffolds exhibited great cytocompatibility and cell recruitment ability in 2- and 4-week subcutaneous implantation experiments and significantly promoted bone regeneration in the periodontal defect scaffold implantation experiment. Moreover, LPA-loaded scaffolds were confirmed to enhance osteogenic activities by upregulating the expression of β-catenin and further activating the Wnt/β-catenin pathway. These results demonstrate that the biphase PP-pDA/nHA-LPA delivery system is a promising material for the GBR.

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Section: Resultsmentioning

confidence: 99%