Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization

Bates, Ryan; Fees, Colby P.; Holland, William L.; Winger, Courtney C.; Khulan, Batbayar; Ancar, Rachel; Bergren, Todd; Petcoff, Douglas W.; Stith, Bradley J.

doi:10.1016/j.ydbio.2013.11.006

Cited by 30 publications

(82 citation statements)

References 118 publications

(212 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Xenopus fertilization, sperm activate phospholipase D1b (PLD1b) to produce PA which then binds and activates Src tyrosine kinase (Bates et al, 2014). A new model for Src activation through PA binding to the SH4, SH3 and a unique domain is presented.…”

Section: Regulation Of Xenopus Fertilization Through Multiple Lipid Pmentioning

confidence: 99%

“…As two different PLD inhibitors fully inhibited the increase of PA and Src activation at fertilization, but only partially inhibited the increase in IP 3 mass and [Ca +2 ] i release (the latter, by ~87%), sperm activate a different pathway that induces a delayed, weak [Ca +2 ] i release (Bates et al, 2014). In addition, multiple studies with tyrosine kinase inhibitors or inhibition of PLCγ activation with SH2 peptides do not eliminate the [Ca +2 ] i increase induced by sperm (Runft et al, 1999).…”

Section: Regulation Of Xenopus Fertilization Through Multiple Lipid Pmentioning

confidence: 99%

See 1 more Smart Citation

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Stith

2015

Developmental Biology

Self Cite

View full text Add to dashboard Cite

This review emphasizes how lipids regulate membrane fusion and the proteins involved in three developmental stages: oocyte maturation to the fertilizable egg, fertilization and during first cleavage. Decades of work show that phosphatidic acid (PA) releases intracellular calcium, and recent work shows that the lipid can activate Src tyrosine kinase or phospholipase C during Xenopus fertilization. Numerous reports are summarized to show three levels of increase in lipid second messengers inositol 1,4,5-trisphosphate and sn 1,2-diacylglycerol (DAG) during the three different developmental stages. In addition, possible roles for PA, ceramide, lysophosphatidylcholine, plasmalogens, phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 4,5-bisphosphate, membrane microdomains (rafts) and phosphatidylinositol 3,4,5-trisphosphate in regulation of membrane fusion (acrosome reaction, sperm-egg fusion, cortical granule exocytosis), inositol 1,4,5-trisphosphate receptors, and calcium release are discussed. The role of six lipases involved in generating putative lipid second messengers during fertilization is also discussed: phospholipase D, autotaxin, lipin1, sphingomyelinase, phospholipase C, and phospholipase A2. More specifically, proteins involved in developmental events and their regulation through lipid binding to SH3, SH4, PH, PX, or C2 protein domains is emphasized. New models are presented for PA activation of Src (through SH3, SH4 and a unique domain), that this may be why the SH2 domain of PLCγ is not required for Xenopus fertilization, PA activation of phospholipase C, a role for PA during the calcium wave after fertilization, and that calcium/calmodulin may be responsible for the loss of Src from rafts after fertilization. Also discussed is that the large DAG increase during fertilization derives from phospholipase D production of PA and lipin dephosphorylation to DAG.

show abstract

Section: Regulation Of Xenopus Fertilization Through Multiple Lipid Pmentioning

confidence: 99%

Section: Regulation Of Xenopus Fertilization Through Multiple Lipid Pmentioning

confidence: 99%

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Stith

2015

Developmental Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…laevis [10–13], here we present several pieces of evidence documenting that it is absolutely required. Primarily, we report that both BAPTA and EGTA effectively prevented fertilization and embryonic development in X .…”

Section: Discussionmentioning

confidence: 92%

“…Moreover, it is a widely held belief in the field of reproductive physiology that Ca 2+ is unessential for fertilization in X . laevis [10–13]. …”

Section: Introductionmentioning

confidence: 99%

Extracellular Ca2+ Is Required for Fertilization in the African Clawed Frog, Xenopus laevis

et al. 2017

View full text Add to dashboard Cite

BackgroundThe necessity of extracellular Ca2+ for fertilization and early embryonic development in the African clawed frog, Xenopus laevis, is controversial. Ca2+ entry into X. laevis sperm is reportedly required for the acrosome reaction, yet fertilization and embryonic development have been documented to occur in high concentrations of the Ca2+ chelator BAPTA. Here we sought to resolve this controversy.Methodology/principal findingUsing the appearance of cleavage furrows as an indicator of embryonic development, we found that X. laevis eggs inseminated in a solution lacking added divalent cations developed normally. By contrast, eggs inseminated in millimolar concentrations of BAPTA or EGTA failed to develop. Transferring embryos to varying solutions after sperm addition, we found that extracellular Ca2+ is specifically required for events occurring within the first 30 minutes after sperm addition, but not after. We found that the fluorescently stained sperm were not able to penetrate the envelope of eggs inseminated in high BAPTA, whereas several had penetrated the vitelline envelope of eggs inseminated without a Ca2+ chelator, or with BAPTA and saturating CaCl2. Together these results indicate that fertilization does not occur in high concentrations of Ca2+ chelators. Finally, we found that the jelly coat includes >5 mM of readily diffusible Ca2+.Conclusions/SignificanceTaken together, these data are consistent with requirement of extracellular Ca2+ for fertilization. Based on our findings, we hypothesize that the jelly coat surrounding the egg acts as a reserve of readily available Ca2+ ions to foster fertilization in changing extracellular milieu.

show abstract

“…, thereby reducing the endoplasmic reticulum stress caused by calcium and affecting macrophage endocytosis [4]. Moreover, PA substances can activate protein kinase C and other related pathways, thus affecting the cell cycle arrest and apoptosis [5].…”

Section: Introductionmentioning

confidence: 99%

Dynamic analysis of phospholipid metabolism of mouse macrophages treated with common non-steroidal anti-inflammatory drugs

Peng

Zhao

et al. 2015

Mol Cell Biochem

View full text Add to dashboard Cite

Through studying the changes of the total phospholipid components in mouse macrophages under the inflammatory status and the drug intervention status, we found the targets of non-steroidal anti-inflammatory drugs on phospholipids, thus providing the basis for the targets of in vitro anti-inflammatory effects of non-steroidal anti-inflammatory drugs. After RAW264.7 cells were pretreated with common non-steroidal anti-inflammatory drugs (aspirin and ibuprofen) and, respectively, stimulated with KLA for various periods (0.5, 4, 12, 16, and 24 h), the phospholipids were extracted. The dynamic changes of phospholipids in cells under various stimulations were analyzed with UPLC-Q-TOF-MS technique. Through the statistical analysis of Simca-P, we explored the potential targets of non-steroidal anti-inflammatory drugs on phospholipids. Through the dynamic analysis of phospholipids, we found two biomarkers (PC(17:1/18:1), PA(18:0/18:4)) which might be in vitro intervention inflammatory response targets of non-steroidal anti-inflammatory drugs. The analysis results show that in anti-inflammatory effects, non-steroidal anti-inflammatory drugs can inhibit COX, induce the cellular fatty acid desaturation and the changes of phospholipid components, stimulate free fatty acids, activate calcium ion channels of endoplasmic reticulum, and promote cell endocytosis, thus controlling inflammation and activating cells. Non-steroidal anti-inflammatory drugs can promote endocytosis, alter cell inflammatory response, and activate the process cells, thus realizing the anti-inflammatory effects.

show abstract

Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization

Cited by 30 publications

References 118 publications

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Phospholipase C and D regulation of Src, calcium release and membrane fusion during Xenopus laevis development

Extracellular Ca2+ Is Required for Fertilization in the African Clawed Frog, Xenopus laevis

Dynamic analysis of phospholipid metabolism of mouse macrophages treated with common non-steroidal anti-inflammatory drugs

Contact Info

Product

Resources

About