Activation of the Albino Sterlet <i>Acipenser ruthenus</i> Eggs by <scp>UV</scp>‐Irradiated Bester Hybrid Spermatozoa to Provide Gynogenetic Progeny

Fopp‐Bayat, Dorota; Ocalewicz, Konrad

doi:10.1111/rda.12521

Cited by 19 publications

(16 citation statements)

References 34 publications

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“…Both reports indicate that differences in the expression of the maternal genes primarily result from differences between mothers. In the androgenetic and gynogenetic experiments, the differences in survival rates of embryos developing in eggs originating from different females have been reported by several authors [13][14][15], among others. Similar events were also observed in the gynogenetic experiment described here, where significant differences in the survival rates between gynogenetic DH rainbow trout developing in eggs originated from four gamete donors were shown.…”

Section: Introductionmentioning

confidence: 81%

Preliminary Identification of Candidate Genes Related to Survival of Gynogenetic Rainbow Trout (Oncorhynchus mykiss) Based on Comparative Transcriptome Analysis

Ocalewicz

Gurgul

Polonis

et al. 2020

Animals

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In the present research, the eggs from four rainbow trout females were used to provide four groups of gynogenetic doubled haploids (DHs). The quality of the eggs from different clutches was comparable, however, interclutch differences were observed in the gynogenetic variants of the experiment and the survival of DH specimens from different groups varied from 3% to 57% during embryogenesis. Transcriptome analysis of the eggs from different females exhibited inter-individual differences in the maternal genes’ expression. Eggs originating from females whose gynogenetic offspring had the highest survival showed an increased expression of 46 genes when compared to the eggs from three other females. Eggs with the highest survival of gynogenetic embryos showed an up-regulation of genes that are associated with cell survival, migration and differentiation (tyrosine-protein kinase receptor TYRO3-like gene), triglyceride metabolism (carnitine O-palmitoyltransferase 1 gene), biosynthesis of polyunsaturated fat (3-oxoacyl-acyl-carrier-protein reductase gene), early embryogenic development (protein argonaute-3 gene, leucine-rich repeat-containing protein 3-like gene), 5S RNA binding (ribosome biogenesis regulatory protein homolog) as well as senescence and aging (telomerase reverse transcriptase, TERT gene), among others. Positive correlation between the genotypic efficiency and egg transcriptome profiles indicated that at least some of the differentially expressed genes should be considered as potential candidate genes for the efficiency of gynogenesis in rainbow trout.

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Section: Introductionmentioning

confidence: 81%

Preliminary Identification of Candidate Genes Related to Survival of Gynogenetic Rainbow Trout (Oncorhynchus mykiss) Based on Comparative Transcriptome Analysis

Ocalewicz

Gurgul

Polonis

et al. 2020

Animals

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“…Normally‐fertilized fish larvae possess higher survival rates than do the gynogentic larvae (Chourrout & Quillet, ; Fujioka, ; Kato et al., ; Nagy, Rajki, Horvath, & Csany, ; Peruzzi et al., ; Purdom, ). Semen irradiation and thermal shock to induce the gynogenesis process are main factors in causing a negative impact on the gynogentic larvae survival rate (Fopp‐Bayat & Ocalewicz, ). According to Fujioka (), gynogenetic honmroko (130 dph) fed with live food ( Brachionus spp.…”

Section: Discussionmentioning

confidence: 99%

“…The induction of meiotic gynogenesis of the sterlet was performed following the procedure of Fopp‐Bayat and Ocalewicz (). The gynogenetic larvae were identified according to the procedures described by Fopp‐Bayat and Woznicki () and Fopp‐Bayat and Ocalewicz (). Eggs from the three females (20 g eggs per female) were mixed together and then divided into six equal groups (K1, K2, K3, G1, G2, and G3).…”

Section: Methodsmentioning

confidence: 99%

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Effects of feeding rate on the growth performance of gynogenetic albino sterlet,Acipenser ruthenus(Linnaeus, 1758) larvae

et al. 2017

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Summary The feeding rate effects were studied on the growth performance of gynogenetic diploid larvae of sterlet Acipenser ruthenus during the first 4 weeks of exogenous feeding. The experimental rearing was conducted from 7 to 38 days post‐hatch (dph) in a circulation system. This was set up in four groups with three replicates (440 individuals/replicate), viz: AC‐control larvae fed Artemia sp., CFC‐control larvae fed compound feed, AG‐gynogenetic larvae fed Artemia sp., and CFG‐gynogenetic larvae fed compound feed. The larvae were reared in glass tanks (44 L volume, 10 individuals/L) with the temperature maintained at 18 ± 0.5°C, photoperiod of 12L:12D and water flow regime of 1‐L/min and fed 50%, 25%, 25%, and 9% of their total biomass/day during feeding. Highest TL and WBW of gynogenetic diploid larvae (AG) were observed with 50.6 ± 1.2 mm and 607.3 ± 36.1 mg (n = 30) at 38 dph. Highest TL and WBW of control larvae (AC) were recorded with 49.5 ± 3.8 mm and 600.8 ± 88.0 mg (n = 30), respectively, with 73.1% ± 11.4% survival; the lowest survival rate was at 46.4% ± 7.1% (n = 30) for the CFG group. The results indicate that the gynogenetic and normal larvae of sterlet fed with live food (Artemia nauplii) from 7 dph can achieve higher growth and survivability compared to the larvae fed with formulated test feed. Results of this study suggest that the effective rearing of sterlet larvae from 7 to 38 dph strongly depends upon the types of feed rather than the genome manipulation performed.

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“…Only maternal or only paternal chromosomes inheritance may be analysed at three different levels. The efficacy of the radiation-induced genetic inactivation of the eggs (androgenesis) or spermatozoa (gynogenesis) may be verified easily by application of the gametes originating from albino males or albino females, respectively, as albinism is usually a recessive trait (Ocalewicz et al 2004; Fopp-Bayat and Ocalewicz 2015). Recovery of the diploid state in fish from the androgenetic and gynogenetic experiments must also be confirmed; thus, such studies require the application of tools to confirm the ploidy level of the provided progeny (Fopp-Bayat and Woźnicki 2006).…”

Section: Introductionmentioning

confidence: 99%

Disturbances in the ploidy level in the gynogenetic sterlet Acipenser ruthenus

et al. 2017

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Artificial mitotic gynogenesis, a chromosome set manipulation, is applied to provide the homozygous progeny with only maternal inheritance. Here, gynogenetic development was induced in the sterlet Acipenser ruthenus L. (Acipenseridae) by activation of the eggs originating from albino females with the UV-irradiated spermatozoa from wild-coloured males, followed by the heat shock applied to suppress the first mitotic division in the haploid zygotes. All experimentally obtained gynogenetic offspring possessed recessive albino coloration. Moreover, the genetic verification, based on three microsatellite DNA markers, confirmed the only maternal inheritance in the albino progeny. Cytogenetic screening enabled identification of the aneuploids, haploids, diploids, triploids, tetraploids and mosaic individuals among the gynogenetic larvae that hatched from the eggs subjected to the heat shock. Furthermore, 40% of the larvae from the haploid variants of the research that were not exposed to the temperature shock showed the diploid chromosome number. A variation of the ploidy level observed in the gynogenetic sterlets may be the consequence of the spontaneous polyploidisation that occurred in the haploid zygotes. Moreover, observation during embryogenesis showed varied stages of eggs development and the asynchronous cell cleavages that may have resulted in the chromosomal disturbances observed in the gynogenetic sterlets here.

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Activation of the Albino Sterlet Acipenser ruthenus Eggs by UV‐Irradiated Bester Hybrid Spermatozoa to Provide Gynogenetic Progeny

Cited by 19 publications

References 34 publications

Preliminary Identification of Candidate Genes Related to Survival of Gynogenetic Rainbow Trout (Oncorhynchus mykiss) Based on Comparative Transcriptome Analysis

Preliminary Identification of Candidate Genes Related to Survival of Gynogenetic Rainbow Trout (Oncorhynchus mykiss) Based on Comparative Transcriptome Analysis

Effects of feeding rate on the growth performance of gynogenetic albino sterlet,Acipenser ruthenus(Linnaeus, 1758) larvae

Disturbances in the ploidy level in the gynogenetic sterlet Acipenser ruthenus

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