Activation of the General Stress Response of <i>Bacillus subtilis</i> by Visible Light

Steen, Jeroen B. van der; Hellingwerf, Klaas J.

doi:10.1111/php.12499

Cited by 11 publications

(7 citation statements)

References 149 publications

(298 reference statements)

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“…These results suggest that there might be daily changes in the expression of genes involved in sensing environmental changes in B. subtilis. Furthermore, B. subtilis is light sensitive, harboring blue-and red-light photoreceptors (6) that could potentially entrain a circadian clock to the 24-hour day. Together, these reports led us to hypothesize that this Eubacterium might entrain to its environment using light and/or temperature signals like other circadian systems.…”

Section: Introductionmentioning

confidence: 99%

A circadian clock in a nonphotosynthetic prokaryote

Eelderink-Chen¹,

Bosman

Sartor³

et al. 2021

Sci. Adv.

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Circadian clocks create a 24-hour temporal structure, which allows organisms to occupy a niche formed by time rather than space. They are pervasive throughout nature, yet they remain unexpectedly unexplored and uncharacterized in nonphotosynthetic bacteria. Here, we identify in Bacillus subtilis circadian rhythms sharing the canonical properties of circadian clocks: free-running period, entrainment, and temperature compensation. We show that gene expression in B. subtilis can be synchronized in 24-hour light or temperature cycles and exhibit phase-specific characteristics of entrainment. Upon release to constant dark and temperature conditions, bacterial biofilm populations have temperature-compensated free-running oscillations with a period close to 24 hours. Our work opens the field of circadian clocks in the free-living, nonphotosynthetic prokaryotes, bringing considerable potential for impact upon biomedicine, ecology, and industrial processes.

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Section: Introductionmentioning

confidence: 99%

A circadian clock in a nonphotosynthetic prokaryote

Eelderink-Chen¹,

Bosman

Sartor³

et al. 2021

Sci. Adv.

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“…The Bacillus subtilis protein YtvA is the first identified prokaryotic protein with a LOV domain. − The LOV domain binds a flavin mononucleotide (FMN) as its chromophore and absorbs blue light at 448 nm. Blue light excitation of FMN triggers a photocycle involving adduct formation with a nearby cysteine residue.…”

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confidence: 99%

Photochemical Reactions of the LOV and LOV-Linker Domains of the Blue Light Sensor Protein YtvA

et al. 2016

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YtvA is a blue light sensor protein composed of an N-terminal LOV (light-oxygen-voltage) domain, a linker helix, and the C-terminal sulfate transporter and anti-σ factor antagonist domain. YtvA is believed to act as a positive regulator for light and salt stress responses by regulating the σB transcription factor. Although its biological function has been studied, the reaction dynamics and molecular mechanism underlying the function are not well understood. To improve our understanding of the signaling mechanism, we studied the reaction of the LOV domain (YLOV, amino acids 26-127), the LOV domain with its N-terminal extension (N-YLOV, amino acids 1-127), the LOV domain with its C-terminal linker helix (YLOV-linker, amino acids 26-147), and the YLOV domain with the N-terminal extension and the C-terminal linker helix (N-YLOV-linker, amino acids 1-147) using the transient grating method. The signals of all constructs showed adduct formation, thermal diffusion, and molecular diffusion. YLOV showed no change in the diffusion coefficient (D), while the other three constructs showed a significant decrease in D within ∼70 μs of photoexcitation. This indicates that conformational changes in both the N- and C-terminal helices of the YLOV domain indeed do occur. The time constant in the YtvA derivatives was much faster than the corresponding dynamics of phototropins. Interestingly, an additional reaction was observed as a volume expansion as well as a slight increase in D only when both helices were included. These findings suggest that although the rearrangement of the N- and C-terminal helices occurs independently on the fast time scale, this change induces an additional conformational change only when both helices are present.

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“…2), showing MNSH1-9K-1 up to 24-times more sensitivity to a 5/125 (1:25) Al/Ni ppm than to a 125/5 (25:1) Al/Ni ppm mixture. It has been documented that the B. megaterium genome contains open reading frames of genes involved in stress responses, including oxidative stress, osmotic stress, heat shock, and detoxification (Liu et al, 2011), which activations, at least in its most studied partner B. subtilis, overlap between general stress responses and more specific pathways (Young et al, 2013), and are highly intertwined under the control of central regulators like σ B , PerR, and extracytoplasmic sigma factors (Helmann 2002;Helmann et al, 2003;van der Steen & Hellingwerf 2015). The differences in cell survival when B. megaterium is exposed to diverse Ni/Al mixtures may be due to the diverse molecular mechanisms activated under certain metal stress conditions, directly related to the specific combination of Ni and Al concentrations present in the samples.…”

Section: Discussionmentioning

confidence: 99%

Effect of aluminum in Bacillus megaterium nickel resistance and removal capability

Rivas-Castillo¹,

Guatemala-Cisneros²,

Rojas-Avelizapa³

2017

Mexjbiotechnol

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The increasing water pollution by heavy metals is considered an alarming situation worldwide, due to the adverse impact they cause in ecosystems and human health. Although conventional techniques are available to diminish the metal concentration present in water bodies, they offer disadvantages, like inefficient metal removal, toxic sludge generation, and high operating costs. In contrast, biotechnological approaches may render a viable alternative, since they offer lower environmental impacts and operating costs, and also higher removal efficiencies when metals are present in small concentrations. It has been shown that the simultaneous presence of more than one metal can generate synergistic, additive or antagonistic effects, thus affecting their removal, and it has been previously demonstrated that B. megaterium strain MNSH1-9K-1 possesses the ability to remove metals present in liquid and solid wastes. Therefore, the goal of the present work was to study B. megaterium MNSH1-9K-1 Ni resistance and removal properties in liquid medium, and to evaluate the variation of these abilities in the presence of another toxic metal, namely Al, which is also commonly found in liquid wastes. To this end, B. megaterium was grown in LB medium with the addition of Ni and/or Al at diverse concentrations, and both metal resistance and Ni removal capabilities were assayed by viable count, and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), respectively. The results obtained strongly suggest that B. megaterium MNSH1-9K-1 presents more susceptibility to Ni than to Al, and that Ni removal is enhanced by the presence of Al.

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Activation of the General Stress Response of Bacillus subtilis by Visible Light

Cited by 11 publications

References 149 publications

A circadian clock in a nonphotosynthetic prokaryote

A circadian clock in a nonphotosynthetic prokaryote

Photochemical Reactions of the LOV and LOV-Linker Domains of the Blue Light Sensor Protein YtvA

Effect of aluminum in Bacillus megaterium nickel resistance and removal capability

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