Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage

Xiao, Xian-mei; Xu, Yan-mei; Zeng, Ze-xiang; Tan, Xiaoxiao; Liu, Zong-li; Chen, Jianwen; Su, Xiaolu; Chen, Jian-Ye

doi:10.3390/ijms20163860

Cited by 23 publications

(12 citation statements)

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“…Furthermore, melatonin application significantly repressed the transcript level of the DELLA protein-encoding gene GAI (used for GA deactivation or as a GA suppressor) as well as GA catabolic regulating gene ( GA2ox1 and GA2ox2 ) expression ( Figure 6D ). Our findings are also supported by previous works, which noted that the application of GA delays natural or stress-induced leaf senescence ( Fan J. et al, 2018 ; Xiao et al, 2019 ). The supplementation with melatonin increases active GAs under salinity stress in cucumber seedlings by amplifying GA biosynthetic genes ( Zhang N. et al, 2014 ) and delays plant senescence by preventing ROS production and optimizing antioxidant enzyme activities ( Wang et al, 2017 ).…”

Section: Discussionsupporting

confidence: 92%

“…Bioactive GA is an essential element that plays an active role in delaying leaf senescence in plants subjected to stressful environments ( Fan J. et al, 2018 ; Xiao et al, 2019 ). In the present investigation, the content of endogenous GA accumulation along with GA biosynthetic encoding enzyme gene ( GA20ox1 and GA20ox2 ) expression was significantly elevated in melatonin-treated plants under heat stress conditions, and the opposite trend was notified in only heat-stressed seedlings ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…A large number of gibberellins are found in the plant kingdom but a limited version of GAs is proactive and helpful for plant development ( Yamaguchi et al, 1998 ). Beyond other activities, GAs are used to prolong leaf senescence ( Beevers, 1966 ; Whyte and Luckwill, 1966 ; Lü et al, 2014 ; Xiao et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Melatonin Pretreatment Confers Heat Tolerance and Repression of Heat-Induced Senescence in Tomato Through the Modulation of ABA- and GA-Mediated Pathways

et al. 2021

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Heat stress and abscisic acid (ABA) induce leaf senescence, whereas melatonin (MT) and gibberellins (GA) play critical roles in inhibiting leaf senescence. Recent research findings confirm that plant tolerance to diverse stresses is closely associated with foliage lifespan. However, the molecular mechanism underlying the signaling interaction of MT with GA and ABA regarding heat-induced leaf senescence largely remains undetermined. Herein, we investigated putative functions of melatonin in suppressing heat-induced leaf senescence in tomato and how ABA and GA coordinate with each other in the presence of MT. Tomato seedlings were pretreated with 100 μM MT or water and exposed to high temperature (38/28°C) for 5 days (d). Heat stress significantly accelerated senescence, damage to the photosystem and upregulation of reactive oxygen species (ROS), generating RBOH gene expression. Melatonin treatment markedly attenuated heat-induced leaf senescence, as reflected by reduced leaf yellowing, an increased Fv/Fm ratio, and reduced ROS production. The Rbohs gene, chlorophyll catabolic genes, and senescence-associated gene expression levels were significantly suppressed by MT addition. Exogenous application of MT elevated the endogenous MT and GA contents but reduced the ABA content in high-temperature-exposed plants. However, the GA and ABA contents were inhibited by paclobutrazol (PCB, a GA biosynthesis inhibitor) and sodium tungstate (ST, an ABA biosynthesis inhibitor) treatment. MT-induced heat tolerance was compromised in both inhibitor-treated plants. The transcript abundance of ABA biosynthesis and signaling genes was repressed; however, the biosynthesis genes MT and GA were upregulated in MT-treated plants. Moreover, GA signaling suppressor and catabolic gene expression was inhibited, while ABA catabolic gene expression was upregulated by MT application. Taken together, MT-mediated suppression of heat-induced leaf senescence has collaborated with the activation of MT and GA biosynthesis and inhibition of ABA biosynthesis pathways in tomato.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

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Melatonin Pretreatment Confers Heat Tolerance and Repression of Heat-Induced Senescence in Tomato Through the Modulation of ABA- and GA-Mediated Pathways

et al. 2021

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“…The process of plant PCD is strictly mediated by hormonal signals, such as ethylene, ABA, SA and JA [ 32 , 33 ]. In addition, several recent studies have indicated that effects of plant hormones are symbiotic or opposite throughout leaf senescence [ 34 , 35 , 36 ]. The expression trends of SlBAG2 and SlBAG5b after treatment with these four hormones were respectively similar.…”

Section: Discussionmentioning

confidence: 99%

Characterization of SlBAG Genes from Solanum lycopersicum and Its Function in Response to Dark-Induced Leaf Senescence

Wang

Jahan

et al. 2021

Plants

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The Bcl-2-associated athanogene (BAG) family is a group of evolutionarily conserved cochaperones involved in diverse cellular functions. Here, ten putative SlBAG genes were identified in tomato. SlBAG2 and SlBAG5b have the same gene structure and conserved domains, along with highly similar identity to their homologs in Arabidopsis thaliana, Oryza sativa, and Triticum aestivum. The qPCR data showed that BAG2 and BAG5b were highly expressed in stems and flowers. Moreover, both genes were differentially expressed under diverse abiotic stimuli, including cold stress, heat stress, salt treatment, and UV irradiation, and treatments with phytohormones, namely, ABA, SA, MeJA, and ETH. Subcellular localization showed that SlBAG2 and SlBAG5b were located in the cell membrane and nucleus. To elucidate the functions in leaf senescence of BAG2 and BAG5b, the full-length CDSs of BAG2 and BAG5b were cloned, and transgenic tomatoes were developed. Compared with WT plants, those overexpressing BAG2 and BAG5b had significantly increased chlorophyll contents, chlorophyll fluorescence parameters and photosynthetic rates but obviously decreased ROS levels, chlorophyll degradation and leaf senescence related gene expression under dark stress. Conclusively, overexpression SlBAG2 and SlBAG5b could improve the tolerance of tomato leaves to dark stress and delay leaf senescence.

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“…The GA 2-oxidase 2 (GA2OX2) gene, which causes GA inactivation, is up-regulated 18-fold during leaf senescence (van der Graaff et al, 2006 ), suggesting that the decrease in active GA may be a cause of leaf senescence. TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) TF BrTCP21 directly binds the promoter of GA biosynthetic gene BrGA20ox3 , activates its transcription, and delays leaf senescence (Xiao et al, 2019 ). The transcript of BrTCP21 decreases along with leaf senescence, while GA 3 treatment keeps BrTCP21 expression in a higher level, which suggests that the positive feedback loop of GA-BrTCP21-GA plays an important role in leaf senescence.…”

Section: Plant Hormones That Delay Leaf Senescencementioning

confidence: 99%

New Advances in the Regulation of Leaf Senescence by Classical and Peptide Hormones

Huang

Guo

2022

Front. Plant Sci.

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Leaf senescence is the last stage of leaf development, manifested by leaf yellowing due to the loss of chlorophyll, along with the degradation of macromolecules and facilitates nutrient translocation from the sink to the source tissues, which is essential for the plants' fitness. Leaf senescence is controlled by a sophisticated genetic network that has been revealed through the study of the molecular mechanisms of hundreds of senescence-associated genes (SAGs), which are involved in multiple layers of regulation. Leaf senescence is primarily regulated by plant age, but also influenced by a variety of factors, including phytohormones and environmental stimuli. Phytohormones, as important signaling molecules in plant, contribute to the onset and progression of leaf senescence. Recently, peptide hormones have been reported to be involved in the regulation of leaf senescence, enriching the significance of signaling molecules in controlling leaf senescence. This review summarizes recent advances in the regulation of leaf senescence by classical and peptide hormones, aiming to better understand the coordinated network of different pathways during leaf senescence.

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Activation of the Transcription of BrGA20ox3 by a BrTCP21 Transcription Factor Is Associated with Gibberellin-Delayed Leaf Senescence in Chinese Flowering Cabbage during Storage

Cited by 23 publications

References 51 publications

Melatonin Pretreatment Confers Heat Tolerance and Repression of Heat-Induced Senescence in Tomato Through the Modulation of ABA- and GA-Mediated Pathways

Melatonin Pretreatment Confers Heat Tolerance and Repression of Heat-Induced Senescence in Tomato Through the Modulation of ABA- and GA-Mediated Pathways

Characterization of SlBAG Genes from Solanum lycopersicum and Its Function in Response to Dark-Induced Leaf Senescence

New Advances in the Regulation of Leaf Senescence by Classical and Peptide Hormones

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