Activation of the Wnt/β-Catenin Pathway by an Inflammatory Microenvironment Affects the Myogenic Differentiation Capacity of Human Laryngeal Mucosa Mesenchymal Stromal Cells

Yang, Runqin; Yang, Xianghua; Liu, Shiyu; Ming, Leiguo; Zhou, Zhifei; Liang, Yuanyuan; Zhao, Yu; Zhou, Fuxing; Deng, Zhihong; Jin, Yan

doi:10.1089/scd.2017.0200

Cited by 6 publications

(7 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Separate microenvironments reflect multiple differentiation potentials involving mesenchymal stromal cells. For example, an inflammatory microenvironment suppresses myogenic differentiation capability regarding laryngeal mucosa mesenchymal stromal cells [LM‐MSCs] (Yang et al, 2018 ). In the current study, we also found that inflammatory cytokines (TNF‐α and IL‐1β) in MG patients were increased.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7

Lai

Yang

et al. 2021

Cell Biology International

View full text Add to dashboard Cite

Myasthenia gravis (MG) is a disease involving neuromuscular transmission that causes fatigue of skeletal muscles and fluctuating weakness. It has been shown that impairment of myogenic differentiation and myofiber maturation may be the underlying cause of MG. In this study, we detected the abnormal expression of circular RNA (circRNA) using next‐generation sequencing in patients with MG. We then investigated the regulatory mechanism and the relationship among circRNA, microRNA, and messenger RNA using quantitative reverse‐transcription polymerase chain reaction, bioinformatics analysis, and luciferase report analysis. The expression of inflammatory cytokines and regulatory T lymphocytes was shown to be increased. Circ‐FBL was significantly increased in MG patients. Bioinformatics and luciferase report analyses confirmed that miR‐133 and PAX7 were the downstream targets of circ‐FBL. Overexpression of circ‐FBL promoted myoblast proliferation by regulation of miR‐133/PAX7. Taken together, our study showed that upregulation of circ‐FBL promoted myogenic proliferation in patients with MG by regulating miR‐133/PAX7.

show abstract

Section: Discussionmentioning

confidence: 99%

Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7

Lai

Yang

et al. 2021

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…miR‐20b, miR‐296, and Let‐7f have been shown to be involved in the regulation of the WNT signaling pathway and angiogenesis by regulating multiple TGs belonging to these pathways . Specifically, PPP2R1B, MYC, and TNF are known to be key regulators of the WNT signaling pathway and HIF1A, while VEGFA and STAT3 are described as key regulators of angiogenesis and the VEGF signaling pathway . MPP3 is known to play a pivotal role in extracellular matrix restructuring of epithelial cells .…”

Section: Discussionmentioning

confidence: 99%

miR‐20b, miR‐296, and Let‐7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes

Gentile

Lhamyani

Coín‐Aragüez

et al. 2018

Obesity

View full text Add to dashboard Cite

Objective: This study aimed to analyze the potential association of different microRNA (miRNA) molecules with both type 2 diabetes (T2D) and obesity and determine their target genes. Methods: Quantitative PCR was used to analyze the miR-20b, miR-296, and Let-7f levels in human visceral and subcutaneous adipose tissues (ATs) in relation to obesity and T2D, miRTarBase 4.0 was used for validation of target genes, and the Protein Analysis Through Evolutionary Relationships (PANTHER) Classification System and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to annotate the biological processes of the predicted targets. Results: In AT, miR-20b, miR-296, and Let-7f levels were significantly different between normoglycemic subjects and those with T2D. In visceral adipose tissue, miRNA levels were higher in normoglycemic/obesity samples than in T2D/obesity samples. miR-20b, miR-296, and Let-7f target genes that showed significant differences in both ATs in relation to obesity and T2D were CDKN1A, CX3CL1, HIF1A, PPP2R1B, STAT3, and VEGFA. These genes are known to be principally involved in the vascular endothelial growth factor (VEGF) and WNT pathways. Conclusions: This study provides experimental evidence of the possible correlation between AT miR-20b, miR-296, and Let-7f with obesity and T2D, which might involve vascular endothelial growth factor and WNTdependent pathways that are regulated by six different genes, suggesting a novel signaling pathway that could be important for understanding the mechanisms underlying the AT dysfunction associated with obesity and T2D.

show abstract

“…Indeed, IL-1β expression levels are significantly higher in OA than in healthy sites and is associated with subchondral destruction 14 . Mechanistically, the inhibitory effects of IL-1β have been associated with MSCs proliferation and the activation of osteoclastogenesis 15 , 16 . IL-1β treatment can also dramatically induce the production of ROS, nitric oxide (NO), and proteolytic enzymes such as matrix metalloproteinase (MMP) during bone formation, thus inhibits BMSCs osteogenic differentiation and bone tissue generation in vitro and in vivo 17 – 19 .…”

Section: Introductionmentioning

confidence: 99%

GABARAP ameliorates IL-1β-induced inflammatory responses and osteogenic differentiation in bone marrow-derived stromal cells by activating autophagy

Guo

2021

Sci Rep

View full text Add to dashboard Cite

Bone mesenchymal stem cells (BMSCs) are the most commonly investigated progenitor cells in bone defect repair and osteoarthritis subchondral bone regeneration; however, these studies are limited by complex inflammatory conditions. In this study, we investigated whether pro-autophagic γ-aminobutyric acid receptor-associated protein (GABARAP) promotes BMSCs proliferation and osteogenic differentiation by modulating autophagy in the presence or absence of interleukin-1 beta (IL-1β) in vitro. The expression levels of all relevant factors were evaluated by qRT-PCR or western blotting where appropriate. BMSCs differentiation were assessed by Alizarin Red, alkaline phosphatase, safranin O, and Oil Red O staining. Furthermore, the interactions between autophagy and osteogenic differentiation were investigated by co-treatment with the autophagy inhibitor 3-methyladenine (3-MA). As the results, we found that treatment with recombinant human His6-GABARAP protein promoted cell proliferation, inhibited apoptosis, and reduced ROS generation by increasing autophagic activity, particularly when co-cultured with IL-1β. Moreover, His6-GABARAP could effectively increase the osteogenic differentiation of BMSCs. The expression levels of inflammatory factors were significantly decreased by His6-GABARAP treatment, whereas its protective effects were attenuated by 3-MA. This study demonstrates that GABARAP maintains BMSCs survival and strengthens their osteogenic differentiation in an inflammatory environment by upregulating mediators of the autophagy pathway.

show abstract

Activation of the Wnt/β-Catenin Pathway by an Inflammatory Microenvironment Affects the Myogenic Differentiation Capacity of Human Laryngeal Mucosa Mesenchymal Stromal Cells

Cited by 6 publications

References 41 publications

Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7

Upregulation of circ‐FBL promotes myogenic proliferation in myasthenia gravis by regulation of miR‐133/PAX7

miR‐20b, miR‐296, and Let‐7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes

GABARAP ameliorates IL-1β-induced inflammatory responses and osteogenic differentiation in bone marrow-derived stromal cells by activating autophagy

Contact Info

Product

Resources

About