2022
DOI: 10.1101/2022.05.11.491493
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Activation of TnSmu1, an integrative and conjugative element, by an ImmR-like transcriptional regulator in Streptococcus mutans

Abstract: Integrative and conjugative elements (ICEs) are chromosomally encoded mobile genetic elements that can transfer DNA between bacterial strains. Recently, as part of efforts to determine hypothetical gene functions, we have discovered an important regulatory module encoded on an ICE known as TnSmu1 on the Streptococcus mutans chromosome. The regulatory module consists of a cI-like repressor with a helix-turn-helix DNA binding domain immRSmu (immunity repressor) and a metalloprotease immASmu (anti-repressor). It … Show more

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Cited by 1 publication
(3 citation statements)
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“…This is consent with excision noted by King, et al . when examining read coverage in an immR Tn Smu1 mutant (King et al, 2022). Together, these data demonstrate that Tn Smu1 is capable of excision from the chromosome of host cells.…”
Section: Resultsmentioning
confidence: 99%
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“…This is consent with excision noted by King, et al . when examining read coverage in an immR Tn Smu1 mutant (King et al, 2022). Together, these data demonstrate that Tn Smu1 is capable of excision from the chromosome of host cells.…”
Section: Resultsmentioning
confidence: 99%
“…However, unlike Tn 916 and ICE clc , our results indicate that Tn Smu1 does not cause host cell death. Additionally, a CRISPRi knockdown of immR Tn Smu1 caused an arrest in growth of the entire population (King et al, 2022). It is possible that the cells with an activated Tn Smu1 die following growth arrest, but we have not observed this, nor have assays measuring death been reported.…”
Section: Discussionmentioning
confidence: 99%
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