Activation of α2 adrenoceptors inhibited NMDA receptor-mediated nociceptive transmission in spinal dorsal horn of mice with inflammatory pain

Fan, Qingqing; Lu, Li; Wang, Wentao; Yang, Xian; Suo, Zhan-Wei; Hu, Xiaodong

doi:10.1016/j.neuropharm.2013.09.024

Cited by 27 publications

(24 citation statements)

References 42 publications

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“…This increase in responsiveness leads to pain hypersensitivity. In the present study, GluN2B was found to be upregulated in RNA and protein levels after sciatic nerve ligation, consistent with the previous studies in different chronic pain models [28–30], which suggests the possibility of using this mechanism as a target for the NP treatment. Previous behavioral studies have shown that systematical application of the selective GluN2B antagonists, ifenprodil and CP-101,606, produce analgesia in animals with persistent inflammatory or neuropathic pain [29, 30].…”

Section: Discussionsupporting

confidence: 92%

Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain

Pan

Chen

et al. 2014

BioMed Research International

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Although neuropathic pain (NP) is still not fully understood by scientists and clinicians alike, studies suggest that N-methyl-D-aspartate (NMDA) receptors play an important role in the induction and maintenance of NP. A promising treatment for NP is through the downregulation of NMDA subunit GluN2B by RNA interference; however, naked siRNA (small interference RNA) is not effective in long-term treatments. In order to concoct a viable prolonged treatment for NP, Lv-siGluN2B (lentivirus carrying siRNA targeting GluN2B subunit) was prepared and the antinociception effects were observed in chronic constriction injury (CCI) rats in the present study. Results showed that Lv-siGluN2B was transduced into spinal cord cells after intrathecal injections and effectively reduced the nociception induced by sciatic nerve ligation while inhibiting the mRNA and protein expression of GluN2B. This antinociception effect lasted approximately 7 weeks. These findings suggest that GluN2B subunit could be a target for NP treatment and Lv-siGluN2B represents a new potential option for long-term treatment of NP.

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Section: Discussionsupporting

confidence: 92%

Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain

Pan

Chen

et al. 2014

BioMed Research International

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“…Based on the many different locations detailed above, there is a wide range of possibilities for novel agents to have an impact. Modulating preand postsynaptic signaling in spinal cord at the receptor level is under investigation [116]. As the genetics underlying the changes that drive LTP in peripheral and central sites become better understood, ERK1, ERK2 and MeCP2 genes will become targets for individualized pain therapy [117].…”

Section: Future Perspectivementioning

confidence: 99%

The Neurobiology of Pain Perception in Normal and Persistent Pain

2015

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Pain is a significant national burden in terms of patient suffering, expenditure and lost productivity. Understanding pain is fundamental to improving evaluation, treatment and innovation in the management of acute and persistent pain syndromes. Pain perception begins in the periphery, and then ascends in several tracts, relaying at different levels. Pain signals arrive in the thalamus and midbrain structures which form the pain neuromatrix, a constantly shifting set of networks and connections that determine conscious perception. Several cortical regions become active simultaneously during pain perception; activity in the cortical pain matrix evolves over time to produce a complex pain perception network. Dysfunction at any level has the potential to produce unregulated, persistent pain.

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“…Inflammation may promote a series of molecular biological events, resulting in the activation of inflammatory cells from the spinal cord tissues and infiltration of the circulatory system, the release of various pro-inflammatory mediators and neurotoxins, and the generation of oxygen radicals and nitroso compounds, and consequently causing cell damage (7,8).…”

Section: Introductionmentioning

confidence: 99%

Anti‑inflammatory effect of delphinidin on intramedullary spinal pressure in a spinal cord injury rat model

Wang

Zhu

et al. 2017

Exp Ther Med

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Abstract. Delphinidin, a flavonoid polyphenolic compound, is widely found in nature and is used as a food supplement due to its pharmacological activity. The aims of the present study were to examine the anti-inflammatory effect of delphinidin in alleviating spinal cord injury (SCI)-induced inflammation in a rat model and to determine the underlying mechanisms in SCI. The Basso, Beattie, Bresnahan (BBB) scores of rats were assessed to evaluate the effect of delphinidin on the recovery of motor function. ELISA kits were also used to analyze the activities of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2) and caspase-3. In addition, the protein expression levels of nuclear factor (NF)-κB, activator protein 1 (AP-1) and p38-MAPK protein expression were measured using western blot analysis. Treatment with delphinidin significantly increased the BBB scores, as well as inhibited the intramedullary spinal pressure in SCI rats. Delphinidin treatment also significantly suppressed the levels of inflammatory factors and NF-κB protein expression in SCI rats. Finally, treatment with delphinidin significantly inhibited NF-κB stimulation, COX-2 activity, PGE2 production, and AP-1 and p38-MAPK protein expression in SCI rats. These results suggest that the anti-inflammatory effect of delphinidin alleviated inflammation in the SCI rat model via alleviation of the intramedullary spinal pressure through the NF-κB and p38-MAPK signaling pathways.

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Activation of α2 adrenoceptors inhibited NMDA receptor-mediated nociceptive transmission in spinal dorsal horn of mice with inflammatory pain

Cited by 27 publications

References 42 publications

Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain

Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain

The Neurobiology of Pain Perception in Normal and Persistent Pain

Anti‑inflammatory effect of delphinidin on intramedullary spinal pressure in a spinal cord injury rat model

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