Activation pattern of neutrophils from blood of elderly individuals with Candida-related denture stomatitis

Gasparoto, Thaís Helena; Oliveira, Carine Ervolino de; Vieira, Narciso Almeida; Porto, Vinícius Carvalho; Cunha, Fernando; Garlet, Gustavo; Campanelli, Ana Paula; Lara, Vanessa Soares

doi:10.1007/s10096-011-1439-z

Cited by 14 publications

(25 citation statements)

References 44 publications

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“…For example, neutrophils cultured with either Mycobacterium tuberculosis or lipoarabinomann (its major cell wall component) have been shown to release CXCL1 and CXCL8 [34], two chemokines involved in neutrophil recruitment. Similarly, neutrophils release CXCL8 when exposed in vitro to Candida albicans [35], Helicobacter pylori water soluble surface protein [36] and H. pylori neutrophil-activating protein (HP-NAP) [37]. By contrast, phagocytosed Staphylococcus aureus has been shown to reduce the production of CXCL8 by neutrophils, concomitantly with suppressing phosphorylation of nuclear factor-B and accelerating cell death, in this manner favoring its own survival and promoting disease [38].…”

Section: Human Neutrophilsmentioning

confidence: 99%

Neutrophil-derived chemokines on the road to immunity

Tecchio

Cassatella

2016

Seminars in Immunology

205

141

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During recent years, it has become clear that polymorphonuclear neutrophils are remarkably versatile cells, whose functions go far beyond phagocytosis and killing. In fact, besides being involved in primary defense against infections-mainly through phagocytosis, generation of toxic molecules, release of toxic enzymes and formation of extracellular traps-neutrophils have been shown to play a role in finely regulating the development and the evolution of inflammatory and immune responses. These latter neutrophil-mediated functions occur by a variety of mechanisms, including the production of newly manufactured cytokines. Herein, we provide a general overview of the chemotactic cytokines/chemokines that neutrophils can potentially produce, either under inflammatory/immune reactions or during their activation in more prolonged processes, such as in tumors. We highlight recent observations generated from studying human or rodent neutrophils in vitro and in vivo models. We also discuss the biological significance of neutrophil-derived chemokines in the context of infectious, neoplastic and immune-mediated diseases. The picture that is emerging is that, given their capacity to produce and release chemokines, neutrophils exert essential functions in recruiting, activating and modulating the activities of different leukocyte populations.

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Section: Human Neutrophilsmentioning

confidence: 99%

Neutrophil-derived chemokines on the road to immunity

Tecchio

Cassatella

2016

Seminars in Immunology

205

141

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“…There are also indications of reduced apoptosis of neutrophils isolated from aged donors. For example, Gasparoto et al [67] recently described neutrophils from elderly human donors to persist longer in culture without stimulation, and a lower rate of spontaneous apoptosis was noted in a separate study with a similar population of subjects [62]. However, the above findings are in conflict with reports of neutrophils from aged individuals being more susceptible to inflammatory-induced apoptosis and unable to be rescued by cytokines such as GM-CSF, unlike their counterparts in younger individuals [68].…”

Section: Neutrophil Phenotype Change and Emerging Subsetsmentioning

confidence: 99%

Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity

2012

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Leucocytes form the principal cellular components of immunity and inflammation, existing as multiple subsets defined by distinct phenotypic and functional profiles. To date, this has most notably been documented for lymphocytes and monocytes. In contrast, as neutrophils are traditionally considered, to be short-lived, terminally differentiated cells that do not re-circulate, the potential existence of distinct neutrophil subsets with functional and phenotypic heterogeneity has not been widely considered or explored. A growing body of evidence is now challenging this scenario, and there is significant evidence for the existence of different neutrophil subsets under both physiological and pathological conditions. This review will summarize the key findings that have triggered a renewed interest in neutrophil phenotypic changes, both in terms of functional implications and consequences within disease models. Special emphasis will be placed on the potential pro- and anti-inflammatory roles of neutrophil subsets, as indicated by the recent works in models of ischaemia–reperfusion injury, trauma, cancer and sepsis.

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“…Much of this deterioration of immunity in elderly is related to predisposition to infectious diseases, such as caused by Gram-negative microorganisms that are frequent in nursing homes and hospital settings [15,16] and mainly combated by innate immune cells. Although the functions of innate immunity appear to be more preserved during aging than the mechanisms developed by the adaptive immune system [8,17], recent evidence has shown that most innate immune functions are at least partly affected during aging [5-7,18-20]. Thus, changes related to immunosenescence could affect the defense mechanism as a whole; however, this complex process is not yet completely understood.…”

Section: Introductionmentioning

confidence: 99%

Proinflammatory profile of in vitro monocytes in the ageing is affected by lymphocytes presence

et al. 2013

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BackgroundAging is associated with complex and constant remodeling of the immune function, resulting in an increasing susceptibility to infection and others diseases. The infections caused by Gram-negative microorganisms, present in nursing homes and hospitals, constitute one of the most common infections in the elderly, and are mainly combated by innate immune cells. Although the functions of innate immunity seem more preserved during aging than of adaptive immune mechanisms, two systems operate in an integrated way in the body, so that injury in one part of the immune system inevitably affects the other as they are part of a defensive network. The aim of this study was to investigate the in vitro production of proinflammatory (TNF-α, IL-6, IL-1β, CXCL-8 and MCP-1) and anti-inflammatory (TGF-β and IL-10) cytokines by monocytes, stimulated or not (basal) with lipopolysaccharide, from healthy young and elderly subjects. By means of PBMCs, we also studied if cytokine profile is altered in these different patient groups, in the presence of lymphocytes, under the same experimental conditions.ResultsThe monocytes from elderly presented higher basal production of TNF-α, MCP-1 and lower of TGF-β than young monocytes. PBMC showed similar cytokines production, irrespective age or stimulation presence. In the presence of lymphocytes, the spontaneous production of IL-10 was higher and of TGF-β was lower than monocytes, regardless of age. After LPS-stimulation, the presence of lymphocytes resulted in increased IL-6, IL-1β, MCP-1 and IL-10 and decreased CXCL-8 and TGF-β in comparison to pure culture of monocytes from young patients. With age, the same differences were observed, except for CXCL-8 and TGF-β which production was the same between monocytes and PBMC stimulated with LPS.ConclusionThese findings reinforce the systemic state of inflamm-aging frequently reported in elderly and considered a factor of susceptibility to numerous diseases. Still, the cytokine production from just monocytes of the elderly showed alterations, while in the lymphocyte presence not, suggesting an immunomodulator role of lymphocytes on monocytes. In addition, the differences between the production patterns by LPS-stimulated PBMC between young and elderly volunteers can be related with an imbalance in response against Gram-negative bacteria in throughout life.

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Activation pattern of neutrophils from blood of elderly individuals with Candida-related denture stomatitis

Cited by 14 publications

References 44 publications

Neutrophil-derived chemokines on the road to immunity

Neutrophil-derived chemokines on the road to immunity

Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity

Proinflammatory profile of in vitro monocytes in the ageing is affected by lymphocytes presence

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