Activation tagging approach in a model legume, Lotus japonicus

Imaizumi, Reiji; Sato, Shusei; Kameya, Nanako; Nakamura, Ikuo; Nakamura, Yasukazu; Tabata, Satoshi; Ayabe, Shin Ichi; Aoki, Takeshi

doi:10.1007/s10265-005-0231-5

Cited by 29 publications

(13 citation statements)

References 29 publications

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“…However, in some cases, like the lap3 mutant in this study, dominant or semidominant loss-of-function phenotypes of the activation-tagging lines are caused by a reduced transcript level of the gene that is located adjacent to the enhancers. Similar cases have been reported for Z26013L and ACC oxidase genes, as they are located 12 and 11.4 kb from their enhancers, respectively, in activation-tagging lines of Arabidopsis and legume plants (Ichikawa et al 2003;Imaizumi et al 2005).…”

Section: Resultssupporting

confidence: 61%

Identification and molecular characterization of a low acid phosphatase 3 (lap3) mutant based on the screening of an Arabidopsis activation-tagged population

Jin

Won

Jeon

et al. 2010

Plant Biotechnol Rep

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A low acid phosphatase 3 (lap3) mutant was identified and characterized from an Arabidopsis activation-tagged (Weigel) population. The roots of the lap3 plants showed lower acid phosphatase (APase) activity compared to wild-type ones under low-Pi conditions (10 lM Pi). Plasmid rescue experiments revealed that the activation-tagging vector was inserted into the intergenic region between At4g31540 and At4g31550 in the Arabidopsis genome. The genotypic segregation of the lap3 mutation was tightly linked with the phenotypic segregation of root APase activity in the prgeny of lap3. The transcript level of the At4g31520 (SDA1: SEVERE DEPOLYMERIZATION OF ACTIN 1), located 7.4 kb from the CaMV 35S enhancers in the lap3 mutant, was significantly reduced compared to that in the wild type. It was speculated that cellular actin polymerization may be involved in Pi acquisition in higher plants.

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Section: Resultssupporting

confidence: 61%

Identification and molecular characterization of a low acid phosphatase 3 (lap3) mutant based on the screening of an Arabidopsis activation-tagged population

Jin

Won

Jeon

et al. 2010

Plant Biotechnol Rep

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“…It is clear that functional analyses have to be carried out for many more mycorrhiza-regulated genes. Lines with insertions spread over the genome of M. truncatula (Tadege et al 2008), L. japonicum (Imaizumi et al 2005) or rice (Kumar et al 2005) in combination with advanced screening tools will probably help to understand the role of numerous genes in a relatively short time.…”

Section: Post-transcriptomic Researchmentioning

confidence: 99%

Molecular–Physiological Aspects of the AM Symbiosis Post Penetration

Franken

2010

Arbuscular Mycorrhizas: Physiology and Function

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The establishment of an arbuscular mycorrhizal symbiosis is characterised by several stages until the fungus has penetrated the root epidermis. The present chapter deals with the subsequent phase where the fungus colonises the root cortex and, when the different functions of the symbiosis are realised. After summarising the morphological and cytological characteristics of this symbiotic phase, different strategies will be described which have been followed to understand the molecular-physiological basis of the symbiosis. Non-targeted approaches resulted in new hypotheses concerning mycorrhizal functioning based on the identification of differentially expressed transcripts or proteins. Some of these hypotheses were further proofed by various biological techniques as localisation of the gene products or down-regulation of the genes in transgenic plants. In contrast targeted approaches were directed to particular plant and fungal functions. Among those functions, the present chapter will concentrate on the assimilation, the metabolism and the distribution of carbohydrates in the plant and the fungus.

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“…Implementing a gain of function approach tool can circumvent these pitfalls. Misexpression mutants can be generated by transforming plants with multiple enhancer sequences (Imaizumi et al 2005;Jeong et al 2006;Nakazawa et al 2003). With respect to a high oil/protein-producing seed such as soybean, implementing seed-speciWc promoters in activation tagged elements would provide an additional aid for elucidating gene function.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository

Mathieu

Winters

Kong

et al. 2008

Planta

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Soybean is a major crop species providing valuable feedstock for food, feed and biofuel. In recent years, considerable progress has been made in developing genomic resources for soybean, including on-going efforts to sequence the genome. These efforts have identified a large number of soybean genes, most with unknown function. Therefore, a major research priority is determining the function of these genes, especially those involved in agronomic performance and seed traits. One means to study gene function is through mutagenesis and the study of the resulting phenotypes. Transposon-tagging has been used successfully in both model and crop plants to support studies of gene function. In this report, we describe efforts to generate a transposon-based mutant collection of soybean. The Ds transposon system was used to create activation-tagging, gene and enhancer trap elements. Currently, the repository houses approximately 900 soybean events, with flanking sequence data derived from 200 of these events. Analysis of the insertions revealed approximately 70% disrupted known genes, with the majority matching sequences derived from either Glycine max or Medicago truncatula sequences. Among the mutants generated, one resulted in male-sterility and was shown to disrupt the strictosidine synthase gene. This example clearly demonstrates that it is possible to disrupt soybean gene function by insertional mutagenesis and to derive useful mutants by this approach in spite of the tetraploid nature of the soybean genome.

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Activation tagging approach in a model legume, Lotus japonicus

Cited by 29 publications

References 29 publications

Identification and molecular characterization of a low acid phosphatase 3 (lap3) mutant based on the screening of an Arabidopsis activation-tagged population

Identification and molecular characterization of a low acid phosphatase 3 (lap3) mutant based on the screening of an Arabidopsis activation-tagged population

Molecular–Physiological Aspects of the AM Symbiosis Post Penetration

Establishment of a soybean (Glycine max Merr. L) transposon-based mutagenesis repository

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