Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance

Manimaran, P.; Reddy, S. Venkata; Moin, Mazahar; Reddy, M. Raghurami; Yugandhar, Poli; Mohanraj, Soundappan S.; Balachandran, S. M.; Kirti, Pulugurtha Bharadwaja

doi:10.1038/s41598-017-10022-9

Cited by 45 publications

(31 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They have been also found to be involved in physiological processes, for instance, the regulation of endoplasmic reticulum (ER) stress [ 8,32], photosynthesis [33][34][35] and photomorphogenesis. In addition, some NF-Y genes also involve in the response to abiotic stresses [ 18,30,[36][37][38][39][40][41][42][43][44][45][46][47]. In leguminous plants, NF-Ys haven been suggested as important regulators of organogenesis and development of symbiotic root nodules [ 18,48] .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of NF-Y gene family in peanut (Arachis hypogaea L.)

Wan

Luo

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of NF-Y gene family in peanut (Arachis hypogaea L.)

Wan

Luo

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, it requires very few primary transformants to obtain a high frequency of transposition events. The rice OsNF‐YC13 transcription factor subunit, identified using Ac / Ds activation system, revealed significant tolerance against salinity stress (Manimaran et al, ). In rice, the Ribosomal Protein Large ( RPL ) subunit genes , RPL 6 and RPL 23A, were found to be upregulated in activation‐tagged lines.…”

Section: Introductionmentioning

confidence: 99%

Development of a large population of activation‐tagged mutants in an elite indica rice variety

et al. 2019

View full text Add to dashboard Cite

In this study, we have generated more than 12,000 activation-tagged mutants in a high-yielding indica rice variety, 'BPT 5204', employing maize Ac/Ds system. Different transgenic plants obtained were analysed based on expression patterns of green fluorescence protein (GFP), red fluorescence protein (RFP), herbicide (Basta) tolerance and molecular analyses. T 1 seeds of pSQ5 and pSQ5-bar transgenics, when germinated separately on hygromycin (50 mg/L) and phosphinothricin (5 mg/L) containing medium, revealed a segregation of 3 tolerant : 1 susceptible plants. The germinal transposition frequency of Ds element in different T 2 progeny of rice plants was found to be about 18.0%. Different stable tagged mutants exhibited marked increases in plant height, number of tillers, leaf size, panicle size, seed size and number of grains per plant. The overall results indicate that the genes associated with these traits are upregulated by the enhancer element in activation-tagged mutants. As such, the various tagged mutant lines appear promising and serve as a valuable genetic resource for identification of key genes determining different agronomic traits of rice. K E Y W O R D S 4X enhancer, Ac/Ds system, activation tagging, agronomic traits, number of grains per plant, tagged mutants | 329 KOTA eT Al. Expression of genes adjacent on either side of the randomly integrated T-DNA insertion sites most often results in gain-of-function phenotypes owing to enhanced gene expression. The two-component transposon systems of Activator/Dissociation (Ac/Ds) (Chin et al., 1999; van Enckevort et al., 2005; Greco et al., 2003; Kolesnik et al., 2004) and Enhancer-Suppressor Mutator (En/Spm-dSpm) (Greco et al., 2004; Kumar, Wing, & Sundaresan, 2005) from maize have been used to generate insertion mutants in rice. Employing transposon system, a relatively small sample of parental lines can generate a large scale of transposon-tagged population (Wang et al., 2013) and transposon tags can be easily eliminated from the revertants, thereby avoiding the tedious complementation procedure. In Arabidopsis, use of GAL4::GFP enhancer trapping system promoted high GFP expression frequencies of about 30% among T 1 lines (Haseloff, 1998). Activation tagging (AT) is a unique technique adopted to improve the crop productivity potential through generation of diverse gain-of-function mutants exhibiting desired attributes. AT mostly results in dominant gain-of-function mutants facilitating enhanced functionality of duplicated genes and thus often generates beneficial attributes amenable for crop improvement. Initially, for studying gain-of-function mutants in plants, enhancer element from the cauliflower mosaic virus (CaMV) 35S gene was utilized (Odell, Nagy, & Chua, 1985). A T-DNA vector was constructed containing four copies of CaMV 35S element and the resultant tetrameric CaMV 35S enhancer could function in either orientation at a con-Government of India.

show abstract

“…Analysis of 70 Ac/Ds rice mutants for salinity tolerance identified one activation-tagged salt tolerant DS plant (DS-16, T3 generation), which showed enhanced expression of a gene encoding the NF-YC subunit, named OsNF-YC13 . The authors identified it as a possible salt stress tolerance gene ( Manimaran et al, 2017 ). In transgenic rice plants, overexpression of a NF-YC gene from Bermuda grass, CdtNF-YC1 , conferred tolerance to drought and salinity ( Chen et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

et al. 2018

View full text Add to dashboard Cite

Two groups of six spring bread wheat varieties with either high or low grain yield under the dry conditions of Central and Northern Kazakhstan were selected for analysis. Experiments were set up with the selected wheat varieties in controlled environments as follows: (1) slowly progressing drought imposed on plants in soil, (2) rapid dehydration of whole plants grown in hydroponics, (3) dehydration of detached leaves, and (4) ABA treatment of whole plants grown in hydroponics. Representatives of two different families of transcription factors (TFs), TaDREB5 and TaNFYC-A7, were found to be linked to yield-under-drought using polymorphic Amplifluor-like SNP marker assays. qRT-PCR revealed differing patterns of expression of these genes in the leaves of plants subjected to the above treatments. Under drought, TaDREB5 was significantly up-regulated in leaves of all high-yielding varieties tested and down-regulated in all low-yielding varieties, and the level of expression was independent of treatment type. In contrast, TaNFYC-A7 expression levels showed different responses in the high- and low-yield groups of wheat varieties. TaNFYC-A7 expression under dehydration (treatments 2 and 3) was higher than under drought (treatment 1) in all high-yielding varieties tested, while in all low-yielding varieties the opposite pattern was observed: the expression levels of this gene under drought were higher than under dehydration. Rapid dehydration of detached leaves and intact wheat plants grown in hydroponics produced similar changes in gene expression. ABA treatment of whole plants caused rapid stomatal closure and a rise in the transcript level of both genes during the first 30 min, which decreased 6 h after treatment. At this time-point, expression of TaNFYC-A7 was again significantly up-regulated compared to untreated controls, while TaDREB5 returned to its initial level of expression. These findings reveal significant differences in the transcriptional regulation of two drought-responsive and ABA-dependent TFs under slowly developing drought and rapid dehydration of wheat plants. The results obtained suggest that correlation between grain yield in dry conditions and TaNFYC-A7 expression levels in the examined wheat varieties is dependent on the length of drought development and/or strength of drought; while in the case of TaDREB5, no such dependence is observed.

show abstract

Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance

Cited by 45 publications

References 89 publications

Genome-wide identification and characterization of NF-Y gene family in peanut (Arachis hypogaea L.)

Genome-wide identification and characterization of NF-Y gene family in peanut (Arachis hypogaea L.)

Development of a large population of activation‐tagged mutants in an elite indica rice variety

Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA

Contact Info

Product

Resources

About