Activators generated electron transfer for atom transfer radical polymerization for immunosensing

Wu, Yafeng; Liu, Songqin

doi:10.1016/j.bios.2010.08.041

Cited by 23 publications

(10 citation statements)

References 44 publications

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“…AGET ATRP is a standalone method to detect biomolecules, but it can also be coupled with other quantitative biodectection tools such as electrochemical (EC) and electrochemiluminescence (ECL) sensors to enable ultrasensitive biodetection. [34][35][36][37][38] Expanding on their earlier works, 34,35 Liu and coworkers demonstrated that various signals tags for EC and ECL-based sensing could be coupled to a great amount of epoxy groups of poly(glycidyl methacrylate) (PGMA) generated by AGET ATRP upon molecular recognition events (Fig. 2C).…”

Section: Atom Transfer Radical Polymerization (Atrp)mentioning

confidence: 92%

“…41 In general, the amplification time could be reduced, but reducing the time would increase the limit of detection. In some cases, post-modification time is much longer than actual polymerization time as in AGET ATRP-based electrochemical biodetection, 34,35,38 where electroactive molecules are coupled to the generated polymer. Recent advances [41][42][43]63,64,68 demonstrate the use of electroactive monomers such as FMMA to reduce the amplification time.…”

Section: Comparison Of Polymerizationbased Biodetection Methods and Future Goalsmentioning

confidence: 99%

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Radical polymerization reactions for amplified biodetection signals

Kim

Sikes

2020

Polym. Chem.

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Section: Atom Transfer Radical Polymerization (Atrp)mentioning

confidence: 92%

Section: Comparison Of Polymerizationbased Biodetection Methods and Future Goalsmentioning

confidence: 99%

Radical polymerization reactions for amplified biodetection signals

Kim

Sikes

2020

Polym. Chem.

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“…These processes cannot be easily extended to industrial production, and do not present substantial improvements over the biosynthetic route. We demonstrate chemical synthesis of this copolymer using Tin(II) 2-ethylhexanoate, a catalyst approved by FDA for the production of biocompatible materials [43,44]. Moreover, we show that this procedure makes it possible to accurately control copolymer composition and molecular weight, and to finely tune its chemico-physical properties and degradation rates.…”

Section: Introductionmentioning

confidence: 96%

Chemical synthesis of a biodegradable PEGylated copolymer from ε-caprolactone and γ-valerolactone: evaluation of reaction and functional properties

et al. 2015

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This paper reports the chemical synthesis of methoxy poly(ethyleneglycol)-block-poly(ε-caprolactone-co-4-hydroxyvalerate) from ε-caprolactone and γ-valerolactone, a five-membered ring rarely used in chemical synthesis due to its low reactivity. This procedure enabled production of copolymers with controlled ratios of repeating units and molecular weights, as demonstrated by GPC, FT-IR and NMR characterization. Copolymer degradation rate was found to depend on macromolecular composition, and finely tuneable in a wide range of values. Similarly, hydrophilicity was dependent on γ-valerolactone content, and could be accurately controlled by varying the composition of the reaction feed. Importantly, this copolymer showed lower levels of acidic degradation products than other biodegradable polymers, thus resulting in improved biocompatibility. These encouraging results demonstrate the feasibility of the chemical synthesis of a novel and versatile material with interesting properties that fill a gap in the range of commercially available biodegradable polymers.

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“…These polymers can be detected by the formation of turbid spots on a sensor, [44][45][46] by turbidity in solution, 47 or by the formation of color or fluorescence and various other means. [48][49][50][51][52][53][54][55][56] In order to achieve polymerization-amplified biosensing and diagnostics, initiators can be linked to recognition motives such as antibodies or DNA fragments. 44,48,[50][51] Alternatively, the catalytic activity of certain biomarkers can be exploited to initiate radical polymerizations, leading to a dual mode of amplification, the catalytic creation of radicals, followed by chain growth polymerization.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytically Initiated Precipitation Atom Transfer Radical Polymerization (ATRP) as a Quantitative Method for Hemoglobin Detection in Biological Fluids

et al. 2019

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The hemoglobin content of blood is an important health indicator, and the presence of microscopic amounts of hemoglobin in places where it normally does not occur, e.g. in blood plasma or in urine, is a sign of diseases such as hemolytic anemia or urinary tract infections. Thus, methods to detect and quantify hemoglobin are important for clinical laboratories, blood banks, and for point-of-care diagnostics. The precipitation polymerization of N-isopropylacrylamide by hemoglobin-catalyzed atom transfer radical polymerization (ATRP) is used as an assay for hemoglobin quantification relying on the formation of turbidity as a simple optical read-out. Dose-response curves for pure hemoglobin, for hemoglobin in blood plasma, in urine, in erythrocytes, and in full blood are obtained. Turbidity formation increases with the concentration of hemoglobin. Concentrations of hemoglobin as low as low as 6.45•10 -3 mg mL -1 in solution, 4.88•10 -1 mg mL -1 in plasma, and 1.65•10 -1 mg mL -1 in urine could be detected, which is below the clinically relevant concentrations in the respective body fluids. Total hemoglobin in full blood is also accurately determined. The reaction can be regarded as a polymerization-based signal amplification for the sensing of hemoglobin, as the analyte catalyzes the formation of radicals which add many monomer units into detectable polymer chains. While most established hemoglobin tests involve the use of highly toxic reagents such as potassium cyanide, the polymerization-based test uses simple and stable organic reagents. Thus, it is an environmentally friendlier alternative to established chemical assays for hemoglobin.

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Activators generated electron transfer for atom transfer radical polymerization for immunosensing

Cited by 23 publications

References 44 publications

Radical polymerization reactions for amplified biodetection signals

Radical polymerization reactions for amplified biodetection signals

Chemical synthesis of a biodegradable PEGylated copolymer from ε-caprolactone and γ-valerolactone: evaluation of reaction and functional properties

Biocatalytically Initiated Precipitation Atom Transfer Radical Polymerization (ATRP) as a Quantitative Method for Hemoglobin Detection in Biological Fluids

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