Active accumulation of very diluted biomolecules by nano-dispensing for easy detection below the femtomolar range

Grilli, Simonetta; Miccio, Lisa; Gennari, Oriella; Coppola, Sara; Vespini, Veronica; Battista, Luigi; Orlando, Pierangelo; Ferraro, Pietro

doi:10.1038/ncomms6314

Cited by 53 publications

(40 citation statements)

References 39 publications

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“…Thanks to the largest known magnitude of spontaneous polarization at room temperature (≈78 μC cm −2 ), LN has been demonstrated to be useful for a wide variety of applications with the key advantage of being electrode‐free, and thus opening the route to more versatile configurations. These include, for example, dielectrophoresis‐based patterning of fluids and particles, nozzle‐free nanodispensing, reshaping, and even biosensing …”

Section: List Of Polymer Solutions Used For Fabricating the Pyroelectmentioning

confidence: 99%

Bipolar Patterning of Polymer Membranes by Pyroelectrification

et al. 2015

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Polymer freestanding membranes with permanent bipolar patterns are fabricated by "pyroelectrification". The thermal stimulation of periodically poled lithium niobate (PPLN) crystals simultaneously generates the pyroelectric effect, the glass transition of the polymer, and therefore the periodic electric poling of the polymer. The reliability of these membranes is demonstrated for applications under both dry and wet conditions, including cell patterning.

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Section: List Of Polymer Solutions Used For Fabricating the Pyroelectmentioning

confidence: 99%

Bipolar Patterning of Polymer Membranes by Pyroelectrification

et al. 2015

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“…Recent advancements in automated diagnostic platforms based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA) have enabled the routine detection of trace levels of nucleic acid and protein biomarker. Additionally, parallel research efforts in sample pre-concentration techniques have shown further potential for enhancement of traditional assays [3]. While these techniques achieve impressive results, all rely heavily upon a complicated sequences of sample preparation and amplification processes that limits their effectiveness outside of well-equipped laboratory environments [4].…”

Section: Introductionmentioning

confidence: 99%

Robust Visualization and Discrimination of Nanoparticles by Interferometric Imaging

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Sevenler

et al. 2017

IEEE J. Select. Topics Quantum Electron.

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Single-molecule and single-nanoparticle biosensors are a growing frontier in diagnostics. Digital biosensors are those which enumerate all specifically immobilized biomolecules or biological nanoparticles, and thereby achieve limits of detection usually beyond the reach of ensemble measurements. Here we review modern optical techniques for single nanoparticle detection and describe the single-particle interferometric reflectance imaging sensor (SP-IRIS). We present challenges associated with reliably detecting faint nanoparticles with SP-IRIS, and describe image acquisition processes and software modifications to address them. Specifically, we describe a image acquisition processing method for the discrimination and accurate counting of nanoparticles that greatly reduces both the number of false positives and false negatives. These engineering improvements are critical steps in the translation of SP-IRIS towards applications in medical diagnostics.

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“…Therefore, triamine/SDS can make the drops uniformly spread on a superhydrophobic surface over a wide range of We , as low as 2 and as high as 614. The uniform round shape with uniform distribution for the solutes is beneficial for highly sensitive detection in bioarrays …”

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confidence: 99%

Uniform Spread of High‐Speed Drops on Superhydrophobic Surface by Live‐Oligomeric Surfactant Jamming

et al. 2019

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self-cleaning and antifouling ability for repelling the deposition of other materials and liquid confining properties for enhancing printing resolution and avoiding coffee-ring effects. [13] However, inertial water drops impacting superhydrophobic surfaces can bounce off quickly or splash violently. [14][15][16][17][18][19][20][21][22][23] Undesired rebound and splash cause material waste [24] and weaken the related performance and efficiency. Many attempts have been conducted to promote water drop spreading on hydrophobic surfaces by using polymers [1,23,[25][26][27][28] or surfactants. [22,[29][30][31][32] However, these two methods still have drawbacks for achieving drop deposition, not to mention uniform spreading: 1) Polymer additives can delay drop retraction but leave drops with hemispherical shape and nonuniform material distribution on the hydrophobic substrate.2) The poor wettability and large mole cular weight of polymer additives restrict the ejecting process during inkjet printing. 3) Surfactant additives can promote drop spreading in a static state owing to the reduced surface tension (γ); [33] however, the low surface tension increases the instability of the impacting drop and leads to drop splashing with satellite droplets, according to the Kelvin-Helmholtz instability, [34] k max ∼ 2ρ a U r 2 /3γ (ρ a is the air density). It is therefore a great challenge for uniform shape spreading on superhydrophobic surfaces without any loss of the drops. Here, we show a new and simple strategy for uniform round-shape drop spreading on superhydrophobic surfaces after high-speed impact, up to 5.0 m s −1 , by utilizing live-oligomeric surfactant jamming, diethylenetriamine/sodium dodecyl sulfate (triamine/SDS). The live-oligomeric surfactant, which noncovalently constructed by SDS and triamine through electrostatic interaction, has a dynamic equilibrium between monomer surfactant and oligomeric surfactant. Figure 1 shows the contrast spread dynamics of a liveoligomeric surfactant drop and other drops impacting superhydrophobic surfaces at an impacting velocity (U ) of 2.42 m s −1 from side and bottom views (Movie S1, Supporting Information). The diameter (D 0 ) of pure water and the surfactant drops is ≈2.25 and 1.90-2.00 mm, respectively (Figure S1, Supporting Information for experimental setup). The Weber number (We), We = ρDV 2 /γ, of water, SDS, N2C3/SDS, triamine/SDS, and 12-3-12-3-12 is 182. 68, 295.29, 358.29, 383.00, and 292.29, respectively. The superhydrophobic surface [35] composed of random micro-nanostructures of typical size and spacing of Inkjet printing of water-based inks on superhydrophobic surfaces is important in high-resolution bioarray detection, chemical analysis, and highperformance electronic circuits and devices. Obtaining uniform spreading of a drop on a superhydrophobic surface is still a challenge. Uniform round drop spreading and high-resolution inkjet printing patterns are demonstrated on superhydrophobic surfaces without splash or rebound after high-speed impacting by introducing...

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Active accumulation of very diluted biomolecules by nano-dispensing for easy detection below the femtomolar range

Cited by 53 publications

References 39 publications

Bipolar Patterning of Polymer Membranes by Pyroelectrification

Bipolar Patterning of Polymer Membranes by Pyroelectrification

Robust Visualization and Discrimination of Nanoparticles by Interferometric Imaging

Uniform Spread of High‐Speed Drops on Superhydrophobic Surface by Live‐Oligomeric Surfactant Jamming

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