Active and Passive Immunizations with<i>Bordetella</i>Colonization Factor A Protect Mice against Respiratory Challenge with<i>Bordetella bronchiseptica</i>

Sukumar, Neelima; Love, Cheraton F.; Conover, Matt S.; Kock, Nancy D.; Dubey, Purnima; Deora, Rajendar

doi:10.1128/iai.01076-08

Cited by 31 publications

(38 citation statements)

References 51 publications

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“…Anti-Bordetella antibodies are critical for resolving Bordetella infections, as well as for vaccine-mediated immune responses (20,25). Previous studies have demonstrated that passive immunization with sera from RB50-infected convalescent mice leads to rapid elimination of B. bronchiseptica from the lower respiratory tract (25,50). Therefore, we investigated if adoptive transfer of sera from LPaV-infected animals provided protection from colonization with wild-type strain RB50 in the mouse respiratory tract.…”

Section: Resultsmentioning

confidence: 99%

“…Immediately following sacrifice, the right lungs were harvested, fixed using neutral buffered formalin for 24 h, trimmed, and processed for histology as described previously (50). Hematoxylin and eosin (H&E)-stained sections were examined by light microscopy for evidence of injury in a blind manner by an American College of Veterinary Pathologists board-certified veterinary pathologist (N.D.K.).…”

Section: Methodsmentioning

confidence: 99%

“…Overnight cultures of the strains were diluted to obtain an optical density at 600 nm (OD 600 ) of 0.05 in 100 l of 0.1 M sodium carbonate-sodium bicarbonate buffer, and the plates were incubated at 4°C overnight. Washing and blocking were carried out as described previously (50). Serum from RB50-or LPaV-infected mice was serially diluted and added to the plates as the primary antibody.…”

Section: Methodsmentioning

confidence: 99%

“…Absorbance at 450 nm was determined using a Labsystems Multiskan Plus plate reader. The OD 450 were plotted against the corresponding dilutions, and endpoint titers were determined as previously described (50).…”

Section: Methodsmentioning

confidence: 99%

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Cross-Species Protection Mediated by a Bordetella bronchiseptica Strain Lacking Antigenic Homologs Present in Acellular Pertussis Vaccines

Sukumar¹,

Sloan²,

Conover

et al. 2010

Infect Immun

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The Bordetella species are Gram-negative bacterial pathogens that are characterized by long-term colonization of the mammalian respiratory tract and are causative agents of respiratory diseases in humans and animals. Despite widespread and efficient vaccination, there has been a world-wide resurgence of pertussis, which remains the leading cause of vaccine-preventable death in developed countries. It has been proposed that current acellular vaccines (Pa) composed of only a few bacterial proteins may be less efficacious because of vaccine-induced antigenic shifts and adaptations. To gain insight into the development of a newer generation of vaccines, we constructed a Bordetella bronchiseptica strain (LPaV) that does not express the antigenic homologs included in any of the Pa vaccines currently in use. This strain also lacks adenylate cyclase toxin, an essential virulence factor, and BipA, a surface protein. While LPaV colonized the mouse nose as efficiently as the wild-type strain, it was highly deficient in colonization of the lower respiratory tract and was attenuated in induction of inflammation and injury to the lungs. Strikingly, to our surprise, we found that in an intranasal murine challenge model, LPaV elicited cross-species protection against both B. bronchiseptica and Bordetella pertussis. Our data suggest the presence of immunogenic protective components other than those included in the pertussis vaccine. Combined with the whole-genome sequences of many Bordetella spp. that are available, the results of this study should serve as a platform for strategic development of the next generation of acellular pertussis vaccines.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cross-Species Protection Mediated by a Bordetella bronchiseptica Strain Lacking Antigenic Homologs Present in Acellular Pertussis Vaccines

Sukumar¹,

Sloan²,

Conover

et al. 2010

Infect Immun

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“…This assay was adapted from a method described previously by Sukumar et al (30). Approximately 2 × 10 6 cfus of S. pneumoniae serotype 14 (ATCC) were incubated with heat-inactivated PPS14-DNI PCMV immune serum (25% or 12.5% serum diluted in PBS), naïve serum (25% serum diluted in PBS), or PBS in a 100-μL volume at 37°C, 5% CO 2 for at least 30 min in wells of a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Conjugate-like immunogens produced as protein capsular matrix vaccines

Thanawastien¹,

Cartee²,

Griffin³

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Capsular polysaccharides are the primary antigenic components involved in protective immunity against encapsulated bacterial pathogens. Although immunization of adolescents and adults with polysaccharide antigens has reduced pathogen disease burden, pure polysaccharide vaccines have proved ineffective at conferring protective immunity to infants and the elderly, age cohorts that are deficient in their adaptive immune responses to such antigens. However, T-cell–independent polysaccharide antigens can be converted into more potent immunogens by chemically coupling to a “carrier protein” antigen. Such “conjugate vaccines” efficiently induce antibody avidity maturation, isotype switching, and immunological memory in immunized neonates. These immune responses have been attributed to T-cell recognition of peptides derived from the coupled carrier protein. The covalent attachment of polysaccharide antigens to the carrier protein is thought to be imperative to the immunological properties of conjugate vaccines. Here we provide evidence that covalent attachment to carrier proteins is not required for conversion of T-independent antigens into T-dependent immunogens. Simple entrapment of polysaccharides or a d-amino acid polymer antigen in a cross-linked protein matrix was shown to be sufficient to produce potent immunogens that possess the key characteristics of conventional conjugate vaccines. The versatility and ease of manufacture of these antigen preparations, termed protein capsular matrix vaccines (PCMVs), will likely provide improvements in the manufacture of vaccines designed to protect against encapsulated microorganisms. This in turn could improve the availability of such vaccines to the developing world, which has shown only a limited capacity to afford the cost of conventional conjugate vaccines.

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Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques

et al. 2007

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This work describes the application of several analytical techniques to characterize the development of Bordetella pertussis biofilms and to examine, in particular, the contribution of virulence factors in this development. Growth of surface-attached virulent and avirulent B. pertussis strains was monitored in continuous-flow chambers by techniques such as the crystal violet method, and nondestructive methodologies like fluorescence microscopy and Fourier transform (FT) IR spectroscopy. Additionally, B. pertussis virulent and avirulent strains expressing green fluorescent protein were grown adhered to the base of a glass chamber of 1-μm thickness. Three-dimensional images of mature biofilms, acquired by confocal laser scanning microscopy, were quantitatively analysed by means of the computer program COMSTAT. Our results indicate that only the virulent (Bvg + ) phase of B. pertussis is able to attach to surfaces and develop a mature biofilm. In the virulent phase these bacteria are capable of producing a biofilm consisting of microcolonies of approximately 200 μm in diameter and 24 μm in depth. FTIR spectroscopy allowed us not only to follow the dynamics of biofilm growth through specific biomass and biofilm marker absorption bands, but also to monitor the maturation of the biofilm by means of the increase of the carbohydrate-to-protein ratio.

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Active and Passive Immunizations withBordetellaColonization Factor A Protect Mice against Respiratory Challenge withBordetella bronchiseptica

Cited by 31 publications

References 51 publications

Cross-Species Protection Mediated by a Bordetella bronchiseptica Strain Lacking Antigenic Homologs Present in Acellular Pertussis Vaccines

Cross-Species Protection Mediated by a Bordetella bronchiseptica Strain Lacking Antigenic Homologs Present in Acellular Pertussis Vaccines

Conjugate-like immunogens produced as protein capsular matrix vaccines

Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques

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