Active Bowman–Birk inhibitors survive gastrointestinal digestion at the terminal ileum of pigs fed chickpea‐based diets

Clemente, Alfonso; Jiménez, Elisabeth; Marín-Manzano, M. Carmen; Rubio, Luis A.

doi:10.1002/jsfa.3115

Cited by 41 publications

(37 citation statements)

References 33 publications

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“…Bowman-Birk inhibitors (BBI) have been shown to be structurally and functionally resistant to the challenges of the GIT in vivo. BBI from chickpea seeds can resist both acidic conditions and the action of proteolytic enzymes, and transit through the stomach and small intestine without major degradation, permitting significant amounts to reach the large intestine in active form [6]. Further studies have demonstrated that the protease inhibitory activities of soybean BBI are unaffected by the metabolic/ proteolytic activities of faecal microbiota, thereby retaining activity potentially linked to CRC-preventive properties [7].…”

Section: Introductionmentioning

confidence: 98%

The cytotoxic effect of Bowman–Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases

Clemente

Moreno

Marín-Manzano

et al. 2010

Molecular Nutrition Food Res

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Bowman-Birk inhibitors (BBI) from soybean and related proteins are naturally occurring protease inhibitors with potential health-promoting properties within the gastrointestinal tract. In this work, we have investigated the effects of soybean BBI proteins on HT29 colon adenocarcinoma cells, compared with non-malignant colonic fibroblast CCD-18Co cells. Two major soybean isoinhibitors, IBB1 and IBBD2, showing considerable amino acid sequence divergence within their inhibitory domains, were purified in order to examine their functional properties, including their individual effects on the proliferation of HT29 colon cancer cells. IBB1 inhibited both trypsin and chymotrypsin whereas IBBD2 inhibited trypsin only. Despite showing significant differences in their enzyme inhibitory properties, the median inhibitory concentration values determined for IBB1 and IBBD2 on HT29 cell growth were not significantly different (39.9+/-2.3 and 48.3+/-3.5 microM, respectively). The cell cycle distribution pattern of HT29 colon cancer cells was affected by BBI treatment in a dose-dependent manner, with cells becoming blocked in the G0-G1 phase. Chemically inactive soybean BBI had a weak but non-significant effect on the proliferation of HT29 cells. The anti-proliferative properties of BBI isoinhibitors from soybean reveal that both trypsin- and chymotrypsin-like proteases involved in carcinogenesis should be considered as potential targets of BBI-like proteins.

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Section: Introductionmentioning

confidence: 98%

The cytotoxic effect of Bowman–Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases

Clemente

Moreno

Marín-Manzano

et al. 2010

Molecular Nutrition Food Res

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“…Trypsin inhibitory activity (TIA) measurements of eluted samples were carried out in flat-bottom microtitre plates by using BAPNA as specific substrate; the assay products were measured at 405 nm, as previously described. 2 Chymotrypsin inhibitory activity (CIA) evaluation of eluted samples was carried out by using BTEE as specific substrate, as previously described. 17 Purified IBB1 and IBBD2 were extensively dialysed against distilled water at 4°C overnight and freeze-dried.…”

Section: Isolation Of Major Soybean Protease Isoinhibitorsmentioning

confidence: 99%

“…BBIs are structurally and functionally resistant to the challenges, acidic conditions and the action of proteolytic enzymes, of the gastrointestinal tract in vivo. 2 The conformational rigidity of BBI linked to the number and distribution of intramolecular disulphide bonds is mostly responsible for the high stability of these proteins towards extreme conditions and helps to maintain the structural and functional features of their binding loops. [3][4][5] Colorectal cancer is the third leading cause of cancerrelated death in the United States and emerging evidence supports that increased consumption of legumes can reduce risk.…”

Section: Introductionmentioning

confidence: 99%

Glycation affects differently the main soybean Bowman–Birk isoinhibitors, IBB1 and IBBD2, altering their antiproliferative properties against HT29 colon cancer cells

et al. 2019

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“…Así, los inhibidores de serín proteasas de la familia de Bowman-Birk (BBI), presentes en semillas de leguminosas tales como la soja, la lenteja, el guisante y el garbanzo, están siendo evaluados como agentes quimiopreventivos colorectales (Clemente, 2014). Cantidades fisiológicamente relevantes de estos inhibidores de serín proteasas del tipo tripsina y quimotripsina alcanzan el intestino grueso en forma activa debido a su extraordinaria resistencia a condiciones extremas (pH ácido, acción de enzimas digestivas y actividad metabólica/proteolítica de la microbiota intestinal) (Clemente, Jiménez, Marín-Manzano y Rubio, 2008;Marín-Manzano, 2009). Recientemente se ha demostrado que los BBI de guisante ejercen propiedades anti-inflamatorias en un modelo de colitis ulcerosa inducida en ratón (Utrilla et al, 2015).…”

Section: Salud Gastrointestinalunclassified

Aspectos de las legumbres nutricionales y beneficiosos para la salud humana

Delgado-Andrade¹,

Olías²,

Jiménez-López³

et al. 2016

Arbor

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RESUMEN:Las leguminosas constituyen una de las familias botá-nicas más importantes desde el punto de vista nutricional, siendo un componente importante de la dieta mediterránea y esencial en la de numerosos países en desarrollo. Legumbres tales como garbanzo, lenteja, altramuz, guisante y habas son reconocidas como fuentes de proteínas, almidón, fibra, vitaminas y minerales. En este trabajo, quedan incluidas las evidencias científicas relativas al papel que juegan las legumbres en la nutrición humana así como las propiedades preventivas que ejercen algunos de sus componentes bioactivos en enfermedades tales como la diabetes tipo-2, hipercolesterolemia, hipertensión, obesidad, enfermedades inflamatorias y cáncer. Por último, se analiza los datos de consumo de legumbres en los hogares españoles, los cuales demuestran un descenso superior al 60 % en las últimas décadas. Esto debe ser corregido con políticas de promoción de dieta saludable así como por medidas de adaptación al nuevo mercado con la aparición de nuevos productos que vayan orientados a las preferencias actuales del consumidor.PALABRAS CLAVE: legumbres; nutrición humana; compuestos bioactivos; salud; prevención; inflamación; cáncer; diabetes tipo-2; hipercolesterolemia; consumidor. ABSTRACT:The Leguminosae is one of the most important botanical families from a nutritional perspective, constituting one of the main ingredients in the Mediterranean diet and an essential food in developing countries. Grain legumes like chickpea, lentil, lupin, pea and beans are well known sources of proteins, starch, fiber, vitamins and minerals. In this paper, scientific evidence for the role of legumes in human nutrition, as well as the preventive properties of some of their bioactive components in diseases like type-2 diabetes, hypercholesterolemia, hypertension, obesity, inflammatory diseases and cancer, are considered. Finally, data of consumption in Spanish households was analyzed, where a 60% decrease has been observed over the last decades. This trend must be reversed by implementing policies to promote healthy diet and by adapting to a new market by means of developing novel products oriented to consumer preferences.

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Active Bowman–Birk inhibitors survive gastrointestinal digestion at the terminal ileum of pigs fed chickpea‐based diets

Cited by 41 publications

References 33 publications

The cytotoxic effect of Bowman–Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases

The cytotoxic effect of Bowman–Birk isoinhibitors, IBB1 and IBBD2, from soybean (Glycine max) on HT29 human colorectal cancer cells is related to their intrinsic ability to inhibit serine proteases

Glycation affects differently the main soybean Bowman–Birk isoinhibitors, IBB1 and IBBD2, altering their antiproliferative properties against HT29 colon cancer cells

Aspectos de las legumbres nutricionales y beneficiosos para la salud humana

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