Active Digital Microfluidic Paper Chips with Inkjet‐Printed Patterned Electrodes

Ko, Hyojin; Lee, Jumi; Kim, Yong-Jun; Lee, Byeongno; Jung, Chan-Hee; Choi, Jae‐Hak; Kwon, Oh‐Sun; Shin, Kwanwoo

doi:10.1002/adma.201305014

Cited by 137 publications

(104 citation statements)

References 44 publications

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“…[1][2][3][4][5][6] These miniaturized, highly integrated systems have the potential to achieve high-resolution molecular detection, more rapid and multiplexed analyses and reduced reagent sample volume (that is, the μl or even pl range). 2,7 Although innovative and more advanced sensors have been developed for specific biomolecule detection down to approximately fM resolution, [8][9][10][11][12] the nm-μm-sized sensor resolution is not limited by the signal transduction limitation but instead by the lack of appropriate analyte transport in solution that governs the detection time and results in poor sensor performance.…”

Section: Introductionmentioning

confidence: 99%

Concentric manipulation and monitoring of protein-loaded superparamagnetic cargo using magnetophoretic spider web

et al. 2017

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A lab-on-a-chip (LOC) magnetophoretic system for the remotely controllable transport of magnetic particles actuated by thin permalloy magnetic tracks has been developed as a novel architecture composed of radii and spiral tracks resembling a spider web network, where the network tracks have the asymmetric and anisotropic magnetic properties for the directional transportation of particles (cargos). A planar Hall resistance (PHR) sensor is integrated with the web networks, and the manipulation and detection are achieved via superparamagnetic particles with dual functions as a biomolecule cargo for transportation and labels for monitoring. The streptavidin protein-coated magnetic particles are precisely manipulated toward the PHR sensor surface via the radii and spiral tracks by applying an external rotating magnetic field. The stray field was analyzed in terms of the particle coverage on the sensor surface, where the sensor signal linearly varies with the number of particles on the sensor surface. This allows the effective collection of low-density biomolecule carriers to one specific point and monitors the accumulated carriers. The developed novel technology could affect multiple fields, including bioassays, cell manipulation and separation and biomechanics.

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Section: Introductionmentioning

confidence: 99%

Concentric manipulation and monitoring of protein-loaded superparamagnetic cargo using magnetophoretic spider web

et al. 2017

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“…These techniques are inexpensive (printing devices onto paper), the device performances are outstanding, and the manufacturing processes have the potential for scaling up to high-volume manufacturing. This is an exciting advance, but the two previous reports leave much to be desired -one 34 has only been demonstrated for oneplate devices (which have limited functionality -likely not useful for high-performance diagnostic assays), while the other 35 uses the powerful two-plate format (which is wellsuited for high-performance diagnostic assays 36 ), but (a) requires an expensive industrial-grade printer, and (b) the device top plates were formed from conventional, expensive materials used in the cleanroom (indium-tin oxide-coated glass). But the biggest drawback for both of the IJP methods reported previously 34,35 is that the dielectric and hydrophobic layers were applied using cleanroom-techniques (i.e., spincoating and chemical vapour deposition) that are not compatible with a scalable, inexpensive production scheme.…”

Section: Introductionmentioning

confidence: 99%

“…16 There have been various efforts at forming DMF devices using cleanroom-free methods over the years, [30][31][32][33] but the performance of devices generated using these techniques was limited, and the techniques themselves were typically not scalable for mass manufacturing. In a critical breakthrough, two groups recently reported methods for forming devices by inkjet printing 34,35 (IJP). These techniques are inexpensive (printing devices onto paper), the device performances are outstanding, and the manufacturing processes have the potential for scaling up to high-volume manufacturing.…”

Section: Introductionmentioning

confidence: 99%

An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays

Dixon

Fobel

et al. 2016

Lab Chip

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The diagnosis of infectious disease is typically carried out at the point-of-care (POC) using the lateral flow assay (LFA). While cost-effective and portable, LFAs often lack the clinical sensitivity and specificity required for accurate diagnoses. In response to this challenge, we introduce a new digital microfluidic (DMF) platform fabricated using a custom inkjet printing and roll-coating process that is scalable to mass production. The performance of the new devices is on par with that of traditional DMF devices fabricated in a cleanroom, with a materials cost for the new devices of only US $0.63 per device. To evaluate the usefulness of the new platform, we performed a 13-step rubella virus (RV) IgG immunoassay on the inkjet printed, roll-coated devices, which yielded a limit of detection of 0.02 IU mL, well below the diagnostic cut-off of 10 IU mL −1 for RV infection and immunity. We propose that this represents a breakthrough for DMF, lowering the costs to a level such that the new platforms will be an attractive alternative to LFAs for the diagnosis of infectious disease at the POC.

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“…Electrowetting on a dielectric (EWOD) is one the actuation techniques used to manipulate the droplets in both open and closed DMF devices [29]. DMF chips can be fabricated by several methods such as photolithography [30], inkjet printing, and rapid prototyping on paper substrates [31][32][33]. Open and closed DMF systems may each offer some advantages and disadvantages for different applications.…”

Section: Chip Fabricationmentioning

confidence: 99%

Ultra-Portable Smartphone Controlled Integrated Digital Microfluidic System in a 3D-Printed Modular Assembly

et al. 2015

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Portable sensors and biomedical devices are influenced by the recent advances in microfluidics technologies, compact fabrication techniques, improved detection limits and enhanced analysis capabilities. This paper reports the development of an integrated ultraportable, low-cost, and modular digital microfluidic (DMF) system and its successful integration with a smartphone used as a high-level controller and post processing station. Low power and cost effective electronic circuits are designed to generate the high voltages required for DMF operations in both open and closed configurations (from 100 to 800 V). The smartphone in turn commands a microcontroller that manipulate the voltage signals required for droplet actuation in the DMF chip and communicates wirelessly with the microcontroller via Bluetooth module. Moreover, the smartphone acts as a detection and image analysis station with an attached microscopic lens. The holder assembly is fabricated using three-dimensional (3D) printing technology to facilitate rapid prototyping. The holder features a modular design that enables convenient attachment/detachment of a variety of DMF chips to/from an electrical busbar. The electrical circuits, controller and communication system are designed to minimize the power consumption in order to run the device on small lithium ion batteries. Successful controlled DMF operations and a basic colorimetric assay using the smartphone are demonstrated.

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Active Digital Microfluidic Paper Chips with Inkjet‐Printed Patterned Electrodes

Cited by 137 publications

References 44 publications

Concentric manipulation and monitoring of protein-loaded superparamagnetic cargo using magnetophoretic spider web

Concentric manipulation and monitoring of protein-loaded superparamagnetic cargo using magnetophoretic spider web

An inkjet printed, roll-coated digital microfluidic device for inexpensive, miniaturized diagnostic assays

Ultra-Portable Smartphone Controlled Integrated Digital Microfluidic System in a 3D-Printed Modular Assembly

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