Internal combustion engines (ICE), as a consequence of new pollutant emissions standards, need several emission control strategies (and related procedures) such as Exhaust Gases Recirculation (EGR), Diesel/Gasoline Particulate Filter (DPF/GPF), Selective Catalyst Reduction (SCR) that allow them to comply with complete requirements defined on those standards. These strategies provoke faster degradation of the engine oil and one of the most relevant consequences is an increase in soot contamination level. All of these strategies facilitate the soot generation.Consequently, soot is one of the most important contaminants present in the engine oil and its relevance is still increasing. The main and classic technique to measure the content of soot in oil is Thermogravimetric analysis (TGA), but this technique has certain limitations. TGA needs a long specific procedure and has limitations to measure small concentration of soot in oil. Therefore, the design of an alternative technique to quantifying the soot in oil concentration is acquiring relevance. One alternative can be the Fourier-transform infra-red (FTIR) spectroscopy, but it also has limitations related to small concentrations of soot in oil. This work presents an alternative technique based on the Ultraviolet-Visible (UV-Vis) spectroscopy, which will let us to quantify 2 small soot contents in used engine oil samples and avoiding potential interferences from other typical contaminants or related with the own measurement process such as sample cuvette material.