Active flow management in preparative chromatographic separations: A preliminary investigation into enhanced separation using a curtain flow inlet fitting and segmented flow outlet fitting

Abstract

Active flow management in the form of curtain flow sample introduction and segmented outlet flow control has been shown to enable sample to elute through a chromatography column under the principles of the "infinite diameter column". Such an elution process avoids the detrimental effects of the heterogeneity of particle-packed chromatographic columns by injecting the sample directly into the radial core region of the column, thus avoiding wall effects. The process described herein illustrates how the principles of the infinite diameter column can be applied using conventional injection devices suitable for long-term analysis that requires robust protocols. Using this approach, sensitivity in separation was 2.5 times greater than conventional chromatography, yielding a product at twice the concentration. Benefits of curtain flow injection are thus relevant to both preparative-scale and analytical-scale separations.

Using reaction flow chromatography for the analysis of amino acid: Derivatisation with fluorescamine reagent

“…To negate the effects of large post-column dead volumes, and at the same time enable PCD capabilities, Camenzuli et al [9][10][11][12][13][14][15] developed a new type of technique, known as Reaction Flow (RF), specifically designed to improve post-column derivatisation reactions, and also enable multiplexed detection [13][14][15]. These columns have been developed to overcome column bed heterogeneity [9][10][11][12][16][17][18][19][20], specifically the Parallel Segmented Flow column (PSF) [16,21].…”

“…These columns have been developed to overcome column bed heterogeneity [9][10][11][12][16][17][18][19][20], specifically the Parallel Segmented Flow column (PSF) [16,21]. The outlet fitting of the RF column can be used as a mixing chamber, the frit being an efficient mixing device especially since at the column outlet there is still several bars of pressure.…”

A Rapid Screening Analysis of Antioxidant Compounds in Native Australian Food Plants Using Multiplexed Detection with Active Flow Technology Columns

“…This is achieved through a purpose-built four-port end-fitting and a three-component annular frit with an impermeable ring, which separates the wall and central flow regions of the column, without cross-flow, at the outlet of the column [4]. The design advantages of these columns have been comprehensively reviewed [4,5,6,7,8,9,10]. One column design within the AFT suite is termed the Parallel Segmented Flow (PSF) column, which provides the added advantage of enabling multiplexed detection protocols.…”

Curtain Flow Column: Optimization of Efficiency and Sensitivity

“…Column beds are heterogeneous in both the radial direction [9][10][11][12] , and along the column axis 10,[12][13][14][15] . The wall-effect especially is an important contributor to the loss of separation performance 7,[16][17][18] . Shalliker and Ritchie 7 recently reviewed aspects of column bed heterogeneity and hence this need not be discussed here further.…”