Active glucagon-like peptide-1 (GLP-1): Storage of human plasma and stability over time

Marino, L.; Griffo, Ettore; Maione, Simona; Mirabella, Martina

doi:10.1016/j.cca.2011.05.011

Cited by 10 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Insulin concentrations were measured by ELISA (DIAsource ImmunoAssays, Nivelles, Belgium) on a Triturus Analyzer (Diagnostics Grifols, Barcelona, Spain). Active GLP-1 was assayed by a nonradioactive, highly specific sandwich ELISA method (Merck-Millipore, Darmstadt, Germany) that had 100% crossreactivity with active isoforms of GLP-1 (7-36 amide and 7-37 glycine extended) but no reactivity with inactive isoforms (9-36 amide and 9-37 glycine extended), GLP-2 or glucagon [25]. Laboratory analyses were performed blinded to the assigned treatment.…”

Section: Experimental Procedures and Laboratory Methodsmentioning

confidence: 99%

Polyphenol-rich diets improve glucose metabolism in people at high cardiometabolic risk: a controlled randomised intervention trial

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Experimental Procedures and Laboratory Methodsmentioning

confidence: 99%

Polyphenol-rich diets improve glucose metabolism in people at high cardiometabolic risk: a controlled randomised intervention trial

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plasma samples were stored at − 80 °C and rigorously kept at + 4 °C during assay. The collection of blood samples with EDTA/Aprotinin under cooled conditions was appropriate to maintain GLP-1 and ghrelin stability as described by Di Marino et al [8] and Tvarijonaviciute et al [9].…”

Section: Mixed Meal Test (Mmt)mentioning

confidence: 99%

Comparative Effects of Roux-en-Y Gastric Bypass and Sleeve Gastrectomy on Glucose Homeostasis and Incretin Hormones in Obese Type 2 Diabetic Patients: A One-Year Prospective Study

et al. 2016

View full text Add to dashboard Cite

ent insulinotropic polypeptide (GIP) [6] and that the restoration of a more physiologic GI hormone profile brought about by bariatric procedures may contribute to the improvement of glucose homeostasis. Roux-en-Y gastric bypass (RYGB), one of the most widely performed bariatric techniques, creates a gastrojejunal anastomosis so that ingested food moves to the distal small intestine bypassing the duodenum and the proximal jejunum. For these characteristics, it is considered a mixed-malabsorptive procedure. RYGB has proven its clinical efficacy in obese patients with type 2 diabetes for more than 30 years, with a diabetes remission of ~75 % [1]. Vertical sleeve gastrectomy (VSG) is a more recent procedure that involves the excision of most of the stomach while maintaining intestinal anatomy. It is considered a merely restrictive procedure although it is associated with marked reduction of plasma ghrelin concentrations due to the removal of the gastric fundus. VSG results in remarkable weight loss and metabolic improvement, reaching a rate of T2DM Introduction ▼The increasing epidemic of obesity and type 2 diabetes (T2DM) is long recognized as a major healthcare problem. Bariatric surgery has emerged as the most successful therapeutic option for morbid obesity, since it results in remarkable and sustained weight loss and a dramatic improvement of glucose control in patients with T2DM [1]. The improvement/resolution of T2DM is associated with the extent of weight loss and the type of surgery ranging from 55 % after restrictive procedures to 95 % after malabsorptive interventions [2]. The beneficial effects on glucose metabolism occur early after surgery, before any substantial weight loss, suggesting a role of weight loss-independent mechanisms, possibly related to changes in gastrointestinal (GI) hormones in response to ingested nutrients [3][4][5]. There is now ample evidence that both obesity and T2DM are associated with impaired secretion/action of GI hormones, namely glucagon-like peptide 1 (GLP-1) and glucose-depend-

show abstract

“…Some hormones are very labile ex vivo, and negative bias due to proteolysis is frequently seen in the measurement of peptides, such as adrenocorticotropic hormones (ACTH), insulin, osteocalcin, C-peptide, and PTH [ 18 , 19 ]. Although the concentrations of most hormones are measured directly in the serum or plasma, it is necessary for many hormones to treat the blood sample specifically before analysis, as in the case of gastrointestinal peptides measurement [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Interpretation of Hormone Levels in Older Patients: Points for Consideration

Sztefko

Szybowska

2012

International Journal of Endocrinology

View full text Add to dashboard Cite

Blood hormone and tumor marker concentrations are usually determined by immunochemical methods which are based on an unique reaction between antigen and assay capture antibody. Despite the speed and simplicity of assays performance on automatic immunochemistry platforms, the interpretation of final results requires a deep knowledge of method fallibility. General lack of immunoassays standardization, presence of cross-reacting substances in patient’s sample, limitation of free hormones measurement due to abnormal analyte binding protein concentrations, assay interferences due to patient’s autoantibodies, and heterophilic antibodies, as well as proper interpretation of very low- and very high-sample analyte levels, are the main points discussed in respect to hormones and tumor markers measurement in geriatric population.

show abstract

Active glucagon-like peptide-1 (GLP-1): Storage of human plasma and stability over time

Cited by 10 publications

References 6 publications

Polyphenol-rich diets improve glucose metabolism in people at high cardiometabolic risk: a controlled randomised intervention trial

Polyphenol-rich diets improve glucose metabolism in people at high cardiometabolic risk: a controlled randomised intervention trial

Comparative Effects of Roux-en-Y Gastric Bypass and Sleeve Gastrectomy on Glucose Homeostasis and Incretin Hormones in Obese Type 2 Diabetic Patients: A One-Year Prospective Study

Interpretation of Hormone Levels in Older Patients: Points for Consideration

Contact Info

Product

Resources

About