Active metabolites produced by Penicillium chrysogenum IFL1 growing on agro-industrial residues

Lopes, Fernanda Cortez; Tichota, Deise Michele; Sauter, Ismael Pretto; Meira, Stela Maris Meister; Segalin, Jéferson; Rott, Marilise Brittes; Rios, Alessandro de Oliveira; Brandelli, Adriano

doi:10.1007/s13213-012-0532-6

Cited by 21 publications

(17 citation statements)

References 46 publications

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“…Microbial extracts represent a good source of biotechnologically important new molecules. Aspergillus and Penicillium species are known as producers of a large number of useful metabolites, especially those with antimicrobial activity [35]. Indeed, the CCF of A. piperis A/5 exhibited significant activity against P. aphanidermatum.…”

Section: Inhibition Of P Aphanidermatum By a Piperis A/5 Extracellumentioning

confidence: 99%

Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum

Jovičić-Petrović

Jeremić

Vučković

et al. 2016

Arch biol sci (Beogr)

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Adding compost to soil can result in plant disease suppression through the mechanisms of antagonistic action of compost microflora against plant pathogens. The aim of the study was to select effective antagonists of Pythium aphanidermatum from compost, to assess the effect of its extracellular metabolites on the plant pathogen, and to characterize antifungal metabolites. The fungal isolate selected by a confrontation test was identified as Aspergillus piperis A/5 on the basis of morphological features and the internal transcribed spacer (ITS) region, ?-tubulin and calmodulin partial sequences. Liquid chromatography-mass spectroscopy (LC-MS) analysis showed that gluconic and citric acid were the most abundant in the organic culture extract. However, the main antifungal activity was contained in the aqueous phase remaining after the organic solvent extraction. The presence of considerable amounts of proteins in both the crude culture extract as well as the aqueous phase remaining after solvent extraction was confirmed by SDS-PAGE. Isolated Aspergillus piperis A/5 exhibits strong antifungal activity against the phytopathogen Pythium aphanidermatum. It secretes a complex mixture of metabolites consisting of small molecules, including gluconic acid, citric acid and itaconic acid derivatives, but the most potent antifungal activity was associated with proteins resistant to heat and organic solvents. Our findings about the activity and characterization of antagonistic strain metabolites contribute to the understanding of the mechanism of interaction of antifungal metabolites as well as fungal-fungal interaction. The obtained results provide a basis for further application development in agriculture and food processing. [Projekat Ministarstva nauke Republike Srbije, br. TR31080 i br. ON173048, i EU Commission project AREA, br.316004]

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Section: Inhibition Of P Aphanidermatum By a Piperis A/5 Extracellumentioning

confidence: 99%

Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum

Jovičić-Petrović

Jeremić

Vučković

et al. 2016

Arch biol sci (Beogr)

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“…For the MS detection, the conditions for the ESI-MS were used as described by Liu et al [49]. The PG standard having more than 98.0% purity (Sigma- Aldrich, Saint Louis, MO, USA) was used to approve the PG metabolites existence in the extract [50].…”

Section: Methodsmentioning

confidence: 99%

Genetic Modification of mfsT Gene Stimulating the Putative Penicillin Production in Monascus ruber M7 and Exhibiting the Sensitivity towards Precursor Amino Acids of Penicillin Pathway

et al. 2019

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The biosynthesis of penicillin G (PG) is compartmentalized, which forces penicillin and its intermediates to cross the membrane barriers. Although many aspects around the penicillin intermediates traffic system remain unclosed, the transmembrane transporter protein involvement has been only predicted. In the present work, detection of PG and isopenicillin N (IPN) in Monascus ruber M7 was performed and functions of mfst gene as a transporter were investigated by the combination of gene deletion (Δmfst) complementation (ΔmfsT::mfsT) and overexpression (M7::PtrpC-mfsT). While, the feeding of PG pathway precursor side chain and amino acids, i.e., phenylacetic acid, D-valine, and L-cysteine was performed for the interpretation of mfsT gene role as an intermediate transporter. The results showed that, the feeding of phenylacetic acid, D-valine, and L-cysteine possessed a significant effect on morphologies, secondary metabolites (SMs) production of all above-mentioned strains including M. ruber M7. The results of UPLC-MS/MS revealed that, ΔmfsT interrupt the penicillin G (PG) production in M. ruber M7 by blocking the IPN transportation, while PG and IPN produced by the ΔmfsT::mfsT have been recovered the similar levels to those of M. ruber M7. Conclusively, these findings suggest that the M. ruber M7 is, not only a PG producer, but also, indicate that the mfsT gene is supposed to play a key role in IPN intermediate compound transportation during the PG production in M. ruber M7.

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“…Agro-industrial residues as an abundant and inexpensive sources can be used for the production of bioactive metabolites and introduce environmental and economic impacts [39,40]. Penicillium chrysogenum IFL1 was used for the production of bioactive secondary metabolites against bacteria, fungi and amoeba using agro-industrial residues as the sole source for nutrients [41]. Two local strains of Penicillium spp.…”

Section: Antimicrobial Activitiesmentioning

confidence: 99%

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

Shawky¹,

Nagah²,

Ghareeb³

et al. 2019

Journal of Renewable Materials

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Rice straw (RS) collected from Egyptian agricultural environment (Al-Masied village-Alsharqia Governorate-Egypt) was used as substrate for three fungal isolates namely: 4b, 1Asp, Pleurotus ostreatus NRRL 3501 and mixed culture of (4b, 1Asp). Isolates number 4b and 1Asp were identified using the molecular technique (18S rRNA). Isolate 4b and 1Asp, were identified as Trichoderma saturnisporum MN1-EGY and Aspergillus niger MN2-EGY, respectively. Trichoderma saturnisporum MN1-EGY extract exhibited the highest antimicrobial activity compared to the other fungal extracts. Total phenolic, antimicrobial and antioxidant activities were also determined from the ethyl acetate extract of rice straw inoculated by these fungi. The antioxidant scavenging concentration(SC50) values of the tested samples were 88.84, 91.45, 94.65 and 100.0µg/ml, respectively for Pleurotus sp., Aspergiluus niger, Aspergiluus niger + Trichoderma sp., and Trichoderma sp., after 15 days incubation, compared to ascorbic acid as positive control with SC50 value equal to 8.0 µg/ml. Total phenolic contents (TPCs) were maximum in Pleurotus sp. extract and found to be the most polyphenolic enriched sample (380.64 mg GAE/g dry extract) compared to extracts of Aspergiluusniger, Aspergiluus niger + Trichoderma sp., and Trichoderma sp. (261.89, 198.52, and 119.80 mg GAE/g dry extract), respectively. The GC-MS analysis has been also performed for the promising extracts.

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Active metabolites produced by Penicillium chrysogenum IFL1 growing on agro-industrial residues

Cited by 21 publications

References 46 publications

Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum

Aspergillus piperis A/5 from plum-distilling waste compost produces a complex of antifungal metabolites active against the phytopathogen Pythium aphanidermatum

Genetic Modification of mfsT Gene Stimulating the Putative Penicillin Production in Monascus ruber M7 and Exhibiting the Sensitivity towards Precursor Amino Acids of Penicillin Pathway

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

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