Plasmon-induced transparency (PIT), usually observed in plasmonic metamaterial structure, remains an attractive topic for research due to its unique optical properties. However, there is almost no research on using the interaction of plasmonic metamaterial and high refractive index dielectric to realize PIT. Here, we report a novel nanophotonics system that makes it possible to realize PIT based on guided-mode resonance and numerically demonstrate its transmission and reflection characteristics by finite element method simulations. The system is composed of a high refractive-index dielectric material and a two-dimensional metallic photonic crystal with 4-fold asymmetric holes. The interaction mechanism of the proposed structure is analyzed by the coupled-mode theory, and the effects of the parameters on PIT are investigated in detail. In addition, we first consider this PIT phenomenon of such fields on atom trapping (87Rb), and the results show that a stable 3D atom trapping with a tunable range of position of about ~17 nm is achieved. Our work provides a novel, efficient way to realize PIT, and it further broadens the application of plasmonic metamaterial systems.