Archaeoseismic analysis performed in Western Sicily point to deformed archeological remains at Lilybaeum, a Punic coastal city founded in 397 B.C. at the Island’s westernmost edge. Starting from the direct observation of deformed ruins, an interdisciplinary work-strategy, which has included field-structural analysis, drone-shot high-resolution aerial photogrammetry, and geophysical prospecting, was employed to investigate whether the identified deformations may represent the ground effects of a previously unknown large earthquake in the area. Among the unearthed remains, some mosaics and a stone-paved monumental avenue show evidence of tectonic deformation being fractured, folded, and uplifted. Trend of folding and fracturing is consistent with the NNW-SSE oriented tectonic max stress axis to which western Sicily is currently undergoing. Displacement along a fracture deforming the Decumanus Maximus together with the finding of a domino-type directional collapse, enable us to interpret the observed deformation as the ground signature of a coseismic slip. Seismic rupture occurred along a previously unmapped deformation front that well fits in the seismotectonic context of Western Sicily. Measured offset, geophysical prospecting, and age-constraints all point to the possibility that a highly-energetic earthquake nucleated in the area following a coseismic rupture along a NE-SW trending back-verging reverse fault towards the end of the IV century A.D. Since seismic catalogs do not provide evidence of such a large earthquake, the latter might represent a missed event in the historical seismic record. This finding provides constraints to redefine the seismic hazard of Western Sicily, a region where recurrence-time intervals for large earthquakes are still unknown.