Active probing of the mechanical properties of biological and synthetic vesicles

Piontek, Melissa C.; Lira, Rafael B.; Roos, Wouter H.

doi:10.1016/j.bbagen.2019.129486

Cited by 41 publications

(42 citation statements)

References 139 publications

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“…Towards that direction, the Cancer-ID consortium was formed to identify and characterise tdEVs and elucidate their differences from EVs of different origins. Electron microscopy, 60,61 nanoparticle tracking analysis, flow cytometry, 62,63 Raman spectroscopy, 64 surface plasmon resonance 55 and atomic force microscopy 65 have been investigated for the characterisation of tdEVs with or without EpCAM pre-enrichment. Principal component analysis of Raman spectra shows clear discrimination between EVs of cancerous cell and healthy blood cell origin.…”

Section: Discussionmentioning

confidence: 99%

Tumour-derived extracellular vesicles in blood of metastatic cancer patients associate with overall survival

Nanou

Miller²,

Zeune

et al. 2020

Br J Cancer

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BACKGROUND: Circulating tumour cells (CTCs) in blood associate with overall survival (OS) of cancer patients, but they are detected in extremely low numbers. Large tumour-derived extracellular vesicles (tdEVs) in castration-resistant prostate cancer (CRPC) patients are present at around 20 times higher frequencies than CTCs and have equivalent prognostic power. In this study, we explored the presence of tdEVs in other cancers and their association with OS. METHODS:The open-source ACCEPT software was used to automatically enumerate tdEVs in digitally stored CellSearch® images obtained from previously reported CTC studies evaluating OS in 190 CRPC, 450 metastatic colorectal cancer (mCRC), 179 metastatic breast cancer (MBC) and 137 non-small cell lung cancer (NSCLC) patients before the initiation of a new treatment. RESULTS: Presence of unfavourable CTCs and tdEVs is predictive of OS, with respective hazard ratios (HRs) of 2.4 and 2.2 in CRPC, 2.7 and 2.2 in MBC, 2.3 and 1.9 in mCRC and 2.0 and 2.4 in NSCLC, respectively. CONCLUSIONS: tdEVs have equivalent prognostic value as CTCs in the investigated metastatic cancers. CRPC, mCRC, and MBC (but not NSCLC) patients with favourable CTC counts can be further prognostically stratified using tdEVs. Our data suggest that tdEVs could be used in clinical decision-making.

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Section: Discussionmentioning

confidence: 99%

Tumour-derived extracellular vesicles in blood of metastatic cancer patients associate with overall survival

Nanou

Miller²,

Zeune

et al. 2020

Br J Cancer

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“…Atomic Force Microscopy-based Force Spectroscopy (AFM-FS), optical tweezers, etc.) are gaining the central stage 31 .…”

Section: Introductionmentioning

confidence: 99%

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

et al. 2021

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“…Although there are multiple well-established methods for extraction of mechanical information of micrometer-sized vesicles, e.g., fluctuation spectroscopy, micropipette aspiration and membrane tether pulling, AFM is currently the only method allowing for the mechanical characterization of submicrometer-sized vesicle populations (Piontek et al, 2019 ). It provides high resolution images of small vesicles on a single particle level under close-to physiological conditions and allows for performing force spectroscopy analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Although the research field of small vesicles has grown enormously during the last decades, and several attempts have been made to extract their mechanics, the understanding of the mechanical properties of small vesicles remains limited (Piontek et al, 2019 ). This is among others due to the non-standardized experimental procedures and data analysis, including application of different models, e.g., Hertz, Sneddon or thin shell models (Krieg et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

Vorselen

Piontek

Roos

et al. 2020

Front. Mol. Biosci.

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Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (<200 nm) perform essential functions in cell biology and artificial vesicles (liposomes) are used as drug delivery vehicles. Atomic Force Microscopy (AFM) is a powerful technique to study the structural properties of these vesicles. AFM is a well-established technique for imaging at nanometer resolution and for mechanical measurements under physiological conditions. Here, we describe the procedure of AFM imaging and force spectroscopy on small vesicles. We discuss how to image vesicles with minimal structural disturbance, and how to analyze the data for accurate size and shape measurements. In addition, we describe the procedure for performing nanoindentations on vesicles and the subsequent data analysis including mechanical models used for data interpretation.

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Active probing of the mechanical properties of biological and synthetic vesicles

Cited by 41 publications

References 139 publications

Tumour-derived extracellular vesicles in blood of metastatic cancer patients associate with overall survival

Tumour-derived extracellular vesicles in blood of metastatic cancer patients associate with overall survival

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

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