Active repression mechanisms of eukaryotic transcription repressors

Hanna-Rose, Wendy; Hansen, Ulla

doi:10.1016/0168-9525(96)10022-6

Cited by 297 publications

(195 citation statements)

References 36 publications

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“…Repression by Ski cannot be explained by competition with an activator for binding to the GTCT site, because c-Ski linked to an unrelated DNA binding domain is able to repress transcription of a cognate reporter. Therefore, Ski can be classified as an active repressor (35). Mechanisms of active repression include interfering with the activity of a DNA-bound activator (44) and interacting with the basal transcription machinery (45) or with histone deacetylases (46).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional Repression by v-Ski and c-Ski Mediated by a Specific DNA Binding Site

Nicol¹,

Stavnezer²

1998

Journal of Biological Chemistry

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The Ski oncoprotein has been shown to bind DNA and activate transcription in conjunction with other cellular factors. Because tumor cells or myogenic cells were used for those studies, it is not clear that those activities of Ski are related to its transforming ability. In this study, we use a nuclear extract of c-ski-transformed cells to identify a specific DNA binding site for Ski with the consensus sequence GTCTAGAC. We demonstrate that both c-Ski and v-Ski in nuclear extracts are components of complexes that bind specifically to this site. By evaluating the features of the sequence that are critical for binding, we show that binding is cooperative. Although Ski cannot bind to this sequence on its own, we use cross-linking with ultraviolet light to show that Ski binds to this site along with several unidentified cellular proteins. Furthermore, we find that Ski represses transcription either through upstream copies of this element or when brought to the promoter by a heterologous DNA binding domain. This is the first demonstration that Ski acts as a repressor rather than an activator and could provide new insights into regulation of gene expression by Ski.

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Section: Discussionmentioning

confidence: 99%

“…There are a variety of mechanisms by which Ski could repress transcription through the GTCT binding site (34,35). One possibility is that Ski might not have its own repression activity but instead might be repressing transcription through the GTCT binding site by competing with another protein for binding to this sequence.…”

Section: Figmentioning

confidence: 99%

Transcriptional Repression by v-Ski and c-Ski Mediated by a Specific DNA Binding Site

Nicol¹,

Stavnezer²

1998

Journal of Biological Chemistry

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“…The high proline, alanine, and glycine content is associated with transcription activation domains in transcription factors (Mitchell and Tjian, 1989;McDowall et al, 1999). On the other hand, alanine repeats or alanine-rich regions may possess repressive activity (Briata et al, 1995;Hanna-Rose and Hansen, 1996;Maurer et al, 2003). How FOXL2 functions evolved in transcription regulation with expansion of proline-alanine-glycine-rich sequences and polyalanine tail formation remains to be clarified.…”

Section: Features Of Chicken and Mammalian Foxl2mentioning

confidence: 99%

Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Govoroun

Pannetier

Pailhoux

et al. 2004

Developmental Dynamics

192

119

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Mutations in the forkhead transcription factor gene FOXL2 are involved in ovarian failure, which occurs in human BPES syndrome. This syndrome presents a sexually dimorphic expression, specific to the ovary in several vertebrates. We cloned the open reading frame of chicken FOXL2 (cFoxL2) and studied cFoxL2 expression in developing gonads and during adulthood to examine the role of FOXL2 in ovarian differentiation and function in birds. The spatial and temporal dynamics of cFoxL2 and aromatase expression were analyzed in parallel by using real-time quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry in attempt to investigate the possible role of cFoxL2 in the regulation of aromatase. The expression patterns of cFoxL2 and aromatase transcripts were highly correlated during the sex-differentiation period (4.7-12.7 days of incubation). Aromatase and cFoxL2 proteins were colocalized in the medullar part of female gonads on embryonic day 14. Fourteen days after hatching, cFoxL2 protein was mainly detected in granulosa cells of developing follicles. In adult ovary follicular envelopes, apart from granulosa cells, cFoxL2 transcript and protein were detected at lower levels in theca cells where aromatase was present. A high level of cFoxL2 transcription was also observed in maturing and ovulated oocytes. Our results confirm that FoxL2 is an early regulator of ovarian development in birds and may be involved in aromatase transcription regulation. Developmental Dynamics 231:859 -870, 2004.

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“…In Xenopus embryos, Oct-1 protein levels differ at least sixfold between cells in different parts of the embryo . In addition, protein-protein interactions often provide a major key to establishing cell type-specific expression patterns during embryonic development (Goodrich et al, 1996;Gray and Levine, 1996;Hanna-Rose and Hansen, 1996). For example, protein-protein interactions mediated by POU domain proteins lead to either synergistic activation or transcriptional repression of target genes in cells where the interacting transcription factors are co-expressed.…”

Section: Differential Expression and Protein-protein Interactions As mentioning

confidence: 99%

Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo

Veenstra

Peterson-Maduro

et al. 1998

Cell Death Differ

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Oct-1, a member of the POU family of transcription factors, is expressed at relatively high levels in ectodermal and mesodermal cell lineages during early Xenopus embryogenesis . Here we show that overexpression of Oct-1 induces programmed cell death concomitant with the loss of the posterior part of the body axis. Truncated Oct-1 variants, missing either the C-terminal or N-terminal transactivation domain, exhibit a different capacity to cause such developmental defects. Oct-1-induced cell death is rescued in unilaterally injected embryos by non-injected cells, indicative of the non-cell autonomous character of the developmental effects of Oct-1. This was confirmed by marker gene analysis, which showed a significant decrease in brachyury expression, suggesting that Oct-1 interferes with an FGF-type signalling pathway.

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Active repression mechanisms of eukaryotic transcription repressors

Cited by 297 publications

References 36 publications

Transcriptional Repression by v-Ski and c-Ski Mediated by a Specific DNA Binding Site

Transcriptional Repression by v-Ski and c-Ski Mediated by a Specific DNA Binding Site

Isolation of chicken homolog of the FOXL2 gene and comparison of its expression patterns with those of aromatase during ovarian development

Non-cell autonomous induction of apoptosis and loss of posterior structures by activation domain-specific interactions of Oct-1 in the Xenopus embryo

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