Active single cell encapsulation using SAW overcoming the limitations of Poisson distribution

Link, Andreas; McGrath, John S.; Zaimagaoglu, Mustafa; Franke, Thomas

doi:10.1039/d1lc00880c

Cited by 19 publications

(9 citation statements)

References 46 publications

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“…Metal deposition is used to deposit gold onto the LiNbO 3 substrate and S1818 photolithography generates the IDT's structure as described in Link et al 31 …”

Section: Methodsmentioning

confidence: 99%

“…From this an effective acoustic path width can be estimated to be w eff ≈ A Nt ≈ 140 μm with half width amplitude of the acoustical path. 30 Metal deposition is used to deposit gold onto the LiNbO 3 substrate and S1818 photolithography generates the IDT's structure as described in Link et al 31 Microfluidic channels were fabricated using standard twolayer SU8-photolithography and PDMS. In the same step we fabricated the cylindrical micro-lenses by integrating the design into the photomask.…”

Section: Microfluidic-chip Fabricationmentioning

confidence: 99%

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Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance

et al. 2023

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“…Metal deposition is used to deposit gold onto the LiNbO 3 substrate and S1818 photolithography generates the IDT's structure as described in Link et al 31 …”

Section: Methodsmentioning

confidence: 99%

Section: Microfluidic-chip Fabricationmentioning

confidence: 99%

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance

et al. 2023

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“…8,9 Metal deposition is used to deposit gold onto the LiNbO3 substrate and S1818 photolithography generates the IDT's structure as described in Link et. al 27 .…”

Section: Microfluidic-chip Fabricationmentioning

confidence: 98%

Sorting of droplets at kHz rates using absorbance activated acoustic sorting

Richter

Link

McGrath

et al. 2022

Preprint

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Droplet microfluidics allows one to address the ever-increasing demand to screen large libraries of biological samples. Absorbance spectroscopy complements the golden standard of fluorescence detection by label free target identification and providing more quantifiable data. However, this is limited by speed and sensitivity. In this paper we increase the speed of sorting by including acoustofluidics, achieving sorting rates of target droplets of 1 kHz. We improved the devices design for detection of absorbance using fibre-based interrogation of samples with integrated lenses in the microfluidic PDMS device for focusing and collimation of light. This optical improvement reduces the scattering and refraction artefacts, improving the signal quality and sensitivity. The novel design allows us to overcome limitations based on dielectrophoresis sorting, such as droplet size dependency, material and dielectric properties of samples. Our acoustic activated absorbance sorter removes the need for offset dyes or matching oils and sorts about a magnitude faster than current absorbance sorter.

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“…These systems are excited either by bulk acoustic waves (BAW) [27][28][29] or surface acoustic waves (SAW). 30,31 Two key differences between these systems are in terms of how the field is excited and the energy retention within the system. BAW systems excite resonances within the fluid volume in contact with a vibrating structure, typically using a piezoelectric ceramic transducer, creating planar wave reflections between channel walls.…”

Section: Introductionmentioning

confidence: 99%