Recombinant granulocyte colony-stimulating factor (G-CSF),
with
a direct repair effect on injured cardiomyocytes against myocardial
infarction ischemia-reperfusion-injury (IRI), displays a poor effect
owing to the limited cardiac targeting efficacy. There are almost
no reports of nanomaterials that deliver G-CSF to the IRI site. Herein,
we propose a way to protect G-CSF by constructing one layer of nitric
oxide (NO)/hydrogen sulfide (H2S) nanomotors on its outside.
NO/H2S nanomotors with specific chemotactic ability to
high expression of reactive oxygen species (ROS)/induced nitric oxide
synthase (iNOS) at the IRI site can deliver G-CSF to the IRI site
efficiently. Meanwhile, superoxide dismutase is covalently bound to
the outermost part, reducing ROS at the IRI site through a cascade
effect with NO/H2S nanomotors. The synergistic effect between
NO and H2S on the effective regulation of the IRI microenvironment
can not only avoid toxicity caused by excessive concentration of a
single gas but also reduce inflammation level and relieve calcium
overload, so as to promote G-CSF to play a cardioprotective role.