“…Onboard the International Space Station (ISS) biofilm formation [26] and consequently microbial contamination continues to pose mission risks, to crew wellbeing as the opportunistic pathogens in water systems and crew cabin present a serious health threat [27][28][29][30]. On the other hand, formation of biofilms on mechanical systems can seriously challenge the hardware reliability as they can also cause biofouling and material degradation, which can lead to system failure during long term missions [31][32][33]. Especially, that with increasing spacecraft complexity, crew numbers, duration of missions, and multiple flights for each spacecraft, new challenges have arisen for long-term control of microbial contamination and biofilm development in systems reused mission-to-mission, particularly in the water storage/distribution systems [28].…”