Active transport of brilliant blue FCF across the Drosophila midgut and Malpighian tubule epithelia

Livingston, Dawson B.H.; Patel, Hirva; Donini, Andrew; MacMillan, Heath A.

doi:10.1016/j.cbpa.2019.110588

Cited by 9 publications

(8 citation statements)

References 55 publications

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“…This is reminiscent of the overall alteration of controlled epithelial permeability broadly affecting living organisms during ageing 75 . The recent demonstration that the Smurf phenotype is due to increased intestinal permeability but also to decreased Malpighian tubules activity 76 is supportive of organismal functional failure occurring in the Smurf phase. Whether it is what is called multivisceral failure in humans is under investigation, but it might highlight the use of the Smurf model of ageing for the study of other barriers, especially the BBB.…”

Section: Discussionmentioning

confidence: 96%

Smurfness-based two-phase model of ageing helps deconvolve the ageing transcriptional signature

Zane

Bouzid

Besse

et al. 2022

Preprint

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Ageing is a common feature of living organisms, showing shared molecular features called hallmarks of ageing. Usually they are quantified in groups of individuals as a function of their chronological age (time passed since birth) and display continuous and progressive changes. Such approaches are based on the assumption that individuals taken at a given chronological age are biological replicates. However, even in genetically homogeneous and synchronised populations individuals do die at different chronological ages. This highlights the difference between chronological age and biological age, the latter being defined by the actual mortality risk of the organism, reflecting its physiology. The Smurf assay, previously described by Rera and colleagues, allows the identification of individuals at higher risk of death from natural causes amongst a population of a given chronological age. We found that the categorization of individuals as Smurf or non-Smurf, permits to distinguish transcriptional changes associated with either chronological or biological age. We show that transcriptional heterogeneity increases with chronological age, while four out of the six currently defined transcriptional hallmarks of ageing are associated with the biological age of individuals, i.e. their Smurf state. In conclusion, we demonstrate that studying properties of ageing by applying the Smurf classification allows us to differentiate the effect of time from the effect of a physiological response triggering an end-of-life switch (i.e. Smurf phase). More specifically, we show that the ability to isolate a pre-death phase of life in vivo enables us not only to study late life mechanisms preceding death, but also investigate early physiological changes triggering such phase. This allowed the identification of novel pro-longevity genetic interventions. We anticipate that the use of the evolutionary conserved Smurf phenotype in ageing studies will allow significant advances in our comprehension of the underlying mechanisms of ageing.

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Section: Discussionmentioning

confidence: 96%

Smurfness-based two-phase model of ageing helps deconvolve the ageing transcriptional signature

Zane

Bouzid

Besse

et al. 2022

Preprint

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“…1). For both Drosophila [39,65,66] and Locusta [67] it has been shown that epithelial barrier function is reduced at low temperature causing increased leak in cold sensitive species (Fig. 3A).…”

Section: The Influence Of Thermal Acclimation and Adaptation On Osmor...mentioning

confidence: 99%

Osmoregulatory capacity at low temperature is critical for insect cold tolerance

Overgaard

Gerber

Andersen

2021

Current Opinion in Insect Science

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“…This experimental setup of a water or ice bath can also be utilized for the opposite thermal conditions of low temperatures [ 110 , 111 , 112 ].…”

Section: Phenotyping Of D Melanogastermentioning

confidence: 99%

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

et al. 2022

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The model organism Drosophila melanogaster was increasingly applied in nutrition research in recent years. A range of methods are available for the phenotyping of D. melanogaster, which are outlined in the first part of this review. The methods include determinations of body weight, body composition, food intake, lifespan, locomotor activity, reproductive capacity and stress tolerance. In the second part, the practical application of the phenotyping of flies is demonstrated via a discussion of obese phenotypes in response to high-sugar diet (HSD) and high-fat diet (HFD) feeding. HSD feeding and HFD feeding are dietary interventions that lead to an increase in fat storage and affect carbohydrate-insulin homeostasis, lifespan, locomotor activity, reproductive capacity and stress tolerance. Furthermore, studies regarding the impacts of HSD and HFD on the transcriptome and metabolome of D. melanogaster are important for relating phenotypic changes to underlying molecular mechanisms. Overall, D. melanogaster was demonstrated to be a valuable model organism with which to examine the pathogeneses and underlying molecular mechanisms of common chronic metabolic diseases in a nutritional context.

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Active transport of brilliant blue FCF across the Drosophila midgut and Malpighian tubule epithelia

Cited by 9 publications

References 55 publications

Smurfness-based two-phase model of ageing helps deconvolve the ageing transcriptional signature

Smurfness-based two-phase model of ageing helps deconvolve the ageing transcriptional signature

Osmoregulatory capacity at low temperature is critical for insect cold tolerance

Phenotyping of Drosophila Melanogaster—A Nutritional Perspective

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