Actively separated microneedle patch for sustained-release of growth hormone to treat growth hormone deficiency

Yang, Li; Liu, Qingyun; Wang, Xinhui; Gao, Nansha; Li, Xiuzhen; Chen, Hongzhong; Mei, Lin; Zeng, Xiaowei

doi:10.1016/j.apsb.2022.04.015

Cited by 32 publications

(17 citation statements)

References 57 publications

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“…The mild fabrication process improved the stability and bioavailability of rhGH. This microneedle system could deliver the drug sustainably for seven days and provided a comparable effect to daily subcutaneous rhGH administration [ 226 ]. In another investigation, Li and colleagues fabricated separable thermosensitive hydrogel microneedles by crosslinking gelatin and carboxylic end-capped poly( N -isopropylacrylamide).…”

Section: Microneedle Types For Biopharmaceutical Deliverymentioning

confidence: 99%

Microneedle-Mediated Transdermal Delivery of Biopharmaceuticals

Nguyen

2023

Pharmaceutics

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Transdermal delivery provides numerous benefits over conventional routes of administration. However, this strategy is generally limited to a few molecules with specific physicochemical properties (low molecular weight, high potency, and moderate lipophilicity) due to the barrier function of the stratum corneum layer. Researchers have developed several physical enhancement techniques to expand the applications of the transdermal field; among these, microneedle technology has recently emerged as a promising platform to deliver therapeutic agents of any size into and across the skin. Typically, hydrophilic biomolecules cannot penetrate the skin by passive diffusion. Microneedle insertion disrupts skin integrity and compromises its protective function, thus creating pathways (microchannels) for enhanced permeation of macromolecules. Microneedles not only improve stability but also enhance skin delivery of various biomolecules. Academic institutions and industrial companies have invested substantial resources in the development of microneedle systems for biopharmaceutical delivery. This review article summarizes the most recent research to provide a comprehensive discussion about microneedle-mediated delivery of macromolecules, covering various topics from the introduction of the skin, transdermal delivery, microneedles, and biopharmaceuticals (current status, conventional administration, and stability issues), to different microneedle types, clinical trials, safety and acceptability of microneedles, manufacturing and regulatory issues, and the future of microneedle technology.

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Section: Microneedle Types For Biopharmaceutical Deliverymentioning

confidence: 99%

Microneedle-Mediated Transdermal Delivery of Biopharmaceuticals

Nguyen

2023

Pharmaceutics

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“…The results indicate a more sustained hypoglycemic effect in vivo compared to subcutaneous injections. Li et al [120] fabricated active separable MPs based on silk protein, which delayed the release of growth hormone and treated growth hormone deficiency. The effervescent substance (NaHCO 3 ) was added to PAA to form an enthusiastic backing (Figure 5c).…”

Section: Delivery Capacity Of Separable Mpsmentioning

confidence: 99%

mentioning

confidence: 99%

Recent Advances in Multifunctional Microneedle Patches for Wound Healing and Health Monitoring

Pan

Liu

Wang

et al. 2022

Advanced NanoBiomed Research

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Benefiting from the advantages of minimal invasiveness, painlessness, and ease to use, microneedle patches (MPs) have been extensively studied in the biomedical field through a physical penetration enhancement pathway. This study systematically summarizes the preparation methods, function optimization, and diagnostic and therapeutic applications of MPs. First of all, the raw materials and methods commonly used in different preparation processes for MPs are highlighted. Then, the corresponding solutions to the problems facing MPs, such as poor tissue adhesion, weak mechanical strength, and low drug delivery utilization in their development and application, are emphasized, which serve as approaches to improve the performance of MPs. Subsequently, the latest applications of MPs for wound healing and health monitoring in recent years are summarized in‐depth, revealing the unique merits of MPs in diagnosis and treatment. Finally, the key points for the urgent improvement of MPs in clinical translation and commercial application are deliberated, in order to inspire the future direction of multifunctional and intelligent MPs.

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“…It also stimulates the production of insulin-like growth factor 1 (IGF-1), which can accelerate the cell proliferation of keratinocytes and fibroblasts [ 2 , 4 , 9 , 10 ]. However, there is a lack of studies to apply hGH to wound healing treatment due to its high molecular weight and low skin permeability, although some studies have been conducted to improve its skin permeability through the use of absorption enhancers, microneedle, ultrasound, and conjugation with a specific peptide that has permeability through the cell membrane [ 7 , 11 , 12 , 13 , 14 ]. Among them, cell-penetrating peptide-mediated delivery is of particular interest due to their unique ability to translocate across plasma membranes efficiently and carry a wide range of macromolecular cargoes with them.…”

Section: Introductionmentioning

confidence: 99%

Topical Delivery of Cell-Penetrating Peptide-Modified Human Growth Hormone for Enhanced Wound Healing

et al. 2023

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Protein drugs have been emerging as a class of promising therapeutics. However, their topical application has been limited by their high molecular weight and poor permeability to the cell membrane. In this study, we aimed to enhance human growth hormone (hGH) permeability for topical application by conjugation of TAT peptide, a cell-penetrating peptide, to hGH via crosslinker. After TAT was conjugated to hGH, TAT-hGH was purified by affinity chromatography. TAT-hGH significantly increased cell proliferation compared with the control. Interestingly, the effect of TAT-hGH was higher than hGH at the same concentration. Furthermore, the conjugation of TAT to hGH enhanced the permeability of TAT-hGH across the cell membrane without affecting its biological activity in vitro. In vivo, the topical application of TAT-hGH into scar tissue markedly accelerated wound healing. Histological results showed that TAT-hGH dramatically promoted the re-epithelialization of wounds in the initial stage. These results demonstrate TAT-hGH as a new therapeutic potential drug for wound healing treatment. This study also provides a new method for topical protein application via enhancement of their permeability.

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Actively separated microneedle patch for sustained-release of growth hormone to treat growth hormone deficiency

Cited by 32 publications

References 57 publications

Microneedle-Mediated Transdermal Delivery of Biopharmaceuticals

Microneedle-Mediated Transdermal Delivery of Biopharmaceuticals

Recent Advances in Multifunctional Microneedle Patches for Wound Healing and Health Monitoring

Topical Delivery of Cell-Penetrating Peptide-Modified Human Growth Hormone for Enhanced Wound Healing

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