Actividad antifúngica in vitro de voriconazol contra dermatofitos y aislamientos superficiales de Scopulariopsis brevicaulis

Carrillo-Muñoz, Alfonso Javier; Cárdenes, C.D.; Carrillo-Orive, Bartolomé; Rodríguez, Virginia Carbajosa; Valle, Oscar del; Casals, J. Bou; Ezkurra, Pilar Ariadna; Quindós, Guillermo

doi:10.1016/s1130-1406(05)70019-x

Cited by 19 publications

(7 citation statements)

References 23 publications

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“…Though the percentage of agreement was slightly higher than in this study (30 to 100%), they considered 63-dilution step as equal, while in our case a 62-dilution step was considered, which might explain the slight discrepancy. The negative correlation observed between BMD and the Etest against DD, though not significant, was similar to what has been observed in similar studies with other fungi (20)(21)(22)(23). The VIPcheck is equally advantageous as it is highly reproducible, could be produced in house, and can be optimized in a 24-well plate to enhance throughput and also reduce cost.…”

Section: Discussionsupporting

confidence: 84%

Comparison of Disc Diffusion, Etest, and a Modified CLSI Broth Microdilution Method for In Vitro Susceptibility Testing of Itraconazole, Posaconazole, and Voriconazole against Madurella mycetomatis

Nyuykonge

Amelsvoort

Eadie

et al. 2021

Antimicrob Agents Chemother

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For many fungal infections, in vitro susceptibility testing is used to predict if an isolate is resistant or susceptible to the antifungal agent used to treat the fungal infection. For Madurella mycetomatis , the main causative agent of mycetoma, in vitro susceptibility testing currently is not performed on a routine basis. The current in vitro susceptibility testing method is labor intensive and sonication must be done to generate a hyphal inoculum. For endpoint visualization, expensive viability dyes are needed. Here we investigated if the currently used in vitro susceptibility method could be adapted to make it amendable for use in a routine setting which can be used in low income countries, where mycetoma is endemic. First, we developed a methodology in which hyphal fragments can be generated without the need for sonication, by comparing different bead beating methodologies. Next, in vitro susceptibility was assessed using standard broth microdilution assays as well as disc diffusion, E-testing and VIPcheck™ methodologies. We demonstrate that after a hyphal suspension is generated by glass bead beating, disc diffusion, E-testing and VIPcheck™ can be used to determine susceptibility towards itraconazole, posaconazole and voriconazole of Madurella mycetomatis . The MICs found with the E-test were comparable to those obtained with our modified CLSI-based broth microdilution in vitro susceptibility assay for itraconazole and posaconazole. Furthermore, we found an inverse relationship between the zone of inhibition and MIC obtained with E-test and the modified CLSI broth microdilution technique.

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Section: Discussionsupporting

confidence: 84%

Comparison of Disc Diffusion, Etest, and a Modified CLSI Broth Microdilution Method for In Vitro Susceptibility Testing of Itraconazole, Posaconazole, and Voriconazole against Madurella mycetomatis

Nyuykonge

Amelsvoort

Eadie

et al. 2021

Antimicrob Agents Chemother

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“…A proper diagnosis of a causative agent of mycosis is essential to implement appropriate treatment. In the case of S. brevicaulis infection, this is of particular importance, because the species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today and responds more poorly to a variety of treatment methods than dermatophytes [ 27 – 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the genus Scopulariopsis, S. brevicaulis is the most common aetiological agent of infections in humans. Scopulariopsis brevicaulis is considered to be resistant to most antifungal drugs currently available, including amphotericin B and azole compounds [ 27 – 29 ]. The treatment of infections due to this species might be ineffective and invasive infections can directly threaten patient's life.…”

Section: Introductionmentioning

confidence: 99%

21-year retrospective study of the prevalence of Scopulariopsis brevicaulis in patients suspected of superficial mycoses

Macura¹,

Skóra²

2015

pdia

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IntroductionIn the genus Scopulariopsis, Scopulariopsis brevicaulis is the most common aetiological agent of infections in humans. It usually affects nails and is one of the commonest moulds associated with onychomycoses. Other forms of infections (skin, subcutaneous, deep tissues, and disseminated infections) have also been described.AimTo examine the prevalence of S. brevicaulis in clinical materials obtained from patients suspected of keratinized tissues mycoses.Material and methodsThe analysis of the prevalence of S. brevicaulis in clinical specimens was based on mycological test's results carried out for patients who were referred with a suspicion of superficial mycoses to the Department of Mycology, Chair of Microbiology, Jagiellonian University Medical College from 1992 till 2012.ResultsIn the years 1992–2012 16,815 clinical samples (nail scrapings, nail swabs, skin scrapings, skin swabs, hair) were collected. Pathogenic fungi were detected in 7193 samples and S. brevicaulis was present in 255 (3.5%). The prevalence of S. brevicaulis in males and females was comparable. The species was most often isolated from toenails (80%), both from males and females. In the analysed period we observed a decrease in the prevalence of S. brevicaulis. In most cases (60%) S. brevicaulis occurred alone in 40% of S. brevicaulis positive cultures, other fungi were also isolated. The fungi most frequently isolated with S. brevicaulis were Trichophyton rubrum, T. mentagrophytes, Penicillium sp., Candida sp., and Aspergillus sp.ConclusionsScopulariopsis brevicaulis is not a common cause of superficial fungal infections of keratinized tissues, but is a typical mould associated with toenail onychomycosis. A proper identification of this species in onychomycoses is essential for the implementation of effective antifungal therapy.

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“…S. brevicaulis has been reported to be resistant in vitro to almost all the currently used drugs, including amphotericin B (AMB), flucytosine, terbinafine (TRB), caspofungin (CAS), and azole compounds (11)(12)(13)(14)(15). In double-drug combinations, synergy was observed in only a few strains of S. brevicaulis with posaconazole (POS) plus TRB and AMB plus CAS (16).…”

mentioning

confidence: 99%

“…This study examined 27 clinical isolates, most of which were S. brevicaulis. The antifungal susceptibilities of Scopulariopsis and Microascus species had been primarily tested in S. brevicaulis (11,13,14,22). Carrillo-Muñoz et al (7,(22)(23)(24) reported that S. brevicaulis is susceptible or intermediately susceptible to voriconazole, sertaconazole, AMB, and TRB.…”

mentioning

confidence: 99%

In Vitro Triple Combination of Antifungal Drugs against Clinical Scopulariopsis and Microascus Species

Yao

Wan

et al. 2015

Antimicrob Agents Chemother

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bBroth microdilution checkerboard techniques based on the methodology of the Clinical and Laboratory Standards Institute (CLSI) were employed to study the triple antifungal combination of caspofungin, posaconazole, and terbinafine against 27 clinical isolates of Scopulariopsis and Microascus species. Synergy was observed for 26 isolates, whereas antagonism was observed for Scopulariopsis candida in this study.

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Actividad antifúngica in vitro de voriconazol contra dermatofitos y aislamientos superficiales de Scopulariopsis brevicaulis

Cited by 19 publications

References 23 publications

Comparison of Disc Diffusion, Etest, and a Modified CLSI Broth Microdilution Method for In Vitro Susceptibility Testing of Itraconazole, Posaconazole, and Voriconazole against Madurella mycetomatis

Comparison of Disc Diffusion, Etest, and a Modified CLSI Broth Microdilution Method for In Vitro Susceptibility Testing of Itraconazole, Posaconazole, and Voriconazole against Madurella mycetomatis

21-year retrospective study of the prevalence of Scopulariopsis brevicaulis in patients suspected of superficial mycoses

In Vitro Triple Combination of Antifungal Drugs against Clinical Scopulariopsis and Microascus Species

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