Actividad antimicrobiana in vitro de extractos de Jatropha dioica seseé contra bacterias fitopatógenas de tomate

Abstract

vesicatory (Xcv) phytopathogenic bacteria, with extracts of different polarity obtained from roots of Jatropha dioica Seseé (Blood grade), endemic plant species of Mexico valued in traditional medicine for its pharmacological and healing properties. As an indicator of cellular respiration in bacterial cultures exposed to extracts, 2,3,5-triphenyltetrazolium chloride was used. The methanolic, hexane and ethyl acetate extracts showed antimicrobial activity against the phytopathogens Cmm, Pst and Xcv. According to the results, the hexane extract showed the lowest Maximum Average Inhibitory concentration (IC50) against Pst and Xcv (0.5 ± 0.01 and 1.7 ± 0.36 mg mL-1 , respectively), so it was separated by column chromatography. As a result, a white crystalline solid identified spectroscopically as citlalitrione was isolated and purified, said compound also exhibited activity against the Cmm, Pst and Xcv bacteria with an IC50 of 1.0 ± 0.13, 1.0 ± 0.11 and 1.1 ± 0.19 mg mL-1 , respectively. These in vitro results open the possibility of using plant extracts of J. dioica for the control of diseases caused by the bacteria studied in tomato cultivation, offering an alternative to the use of antibiotics or copper compounds.

Content of polyphenolic compounds, flavonoids, antioxidant activity and antibacterial activity of Jatropha dioica hydroalcoholic extracts against Streptococcus mutans

“…In the literature, it is reported that the antibacterial activity is due to the presence of polyphenolic compounds (Mutheeswaran et al ., 2017). Yet, others emphasise the importance of the presence of different components that act synergistically (Pérez‐Pérez et al ., 2020; Alkufeidy et al ., 2022). In previous studies, J. dioica extracts have shown antimicrobial activity against Staphylococcus aureus , Klebsiella pneumoniae , Pseudomonas aeruginosa and Escherichia coli (Serrano‐Gallardo et al ., 2017).…”

“…Jatrophone, rhyolosatrione and citlalitrione are terpenes present in J. dioica . Also, diterpenic compounds could be responsible for this activity because they damage biomembranes due to their lipophilic characteristics (Pérez‐Pérez et al ., 2020; Alkufeidy et al ., 2022). On the other hand, López‐García et al .…”

Efectividad biológica de extractos de Agave striata y Fouquieria splendens contra Clavibacter michiganensis Subp. michiganensis.

“…(2020) con extractos etanólicos de hojas de Magnolia tamaulipana reportando una IC 50 y IC 90 de 64.75 -176.73 ppm;además Arredondo-Valdés et al (2021) utilizaron extractos etanólicos de hojas de Moringa oleifera contra Cmm, obteniendo una IC 50 fue de 350.48 ppm. Pérez-Pérez et al (2020) señalan que los extractos metanólicos, hexánico y de acetato de etilo a partir de raíces de Jatropha dioica Seseé (Sangre de drago), también inhibieron el crecimiento de Cmm, con una mejor respuesta con acetato de etilo con una CI 50 de 2.0 ± 0.43 mg mL-1. De igual manera se reportan con los extractos metanólicos de hojas de Calendula officinalis y Echinacea purpurea actividad inhibidora contra Cmm (Aksoy et al, 2021).…”