Activin effects on follicular growth in &lt;i&gt;in vitro&lt;/i&gt; preantral follicle culture

Tanaka, Yu; Matsuzaki, Takashi; Tanaka, Naoko; Iwasa, Takeshi; Kuwahara, Akira; Irahara, Minoru

doi:10.2152/jmi.66.165

Cited by 11 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3] The 80-180 μm preantral follicle is selected for in vitro culture, 13,22,23 and the commonly used r-FSH concentration is 10 mIU/ mL or 100 mIU/mL. 13,23,24 Under the same conditions, the average in vitro maturation rate of follicular oocytes is 60%-70%. [1][2][3] The in vitro maturation is unstable, and the maturation rate varies greatly (8%-80.88%).…”

Section: Discussionmentioning

confidence: 99%

“…Many factors, including breed, FSH concentration, follicle size, and use of mineral oil, can affect the development of preantral follicles of mice in vitro. Therefore, how to optimize and stabilize the in vitro culture of preantral follicles is continuously being investigated 1,7,11‐14 …”

Section: Introductionmentioning

confidence: 99%

“…Therefore, how to optimize and stabilize the in vitro culture of preantral follicles is continuously being investigated. 1,7,[11][12][13][14] The growth and development of follicles are regulated by hormones. Follicular development includes primordial follicles, primary follicles (PM), secondary follicles, antral follicles, and mature follicles.…”

Section: Introductionmentioning

confidence: 99%

“…al also showed that FSH could promote the survival of preantral follicles with a diameter of 95-142 μm in mice. 21 Recently, preantral follicles with a diameter of 80-180 μm are often selected and used for in vitro culture, 13,22,23 and follicles in this diameter range include grade II, III, IV, and V follicles. The different grades of follicles have different amounts of granulosa cells and different sizes.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The optimized research of the in vitro culture of preantral follicles in mice

Wang

Sun

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The optimized research of the in vitro culture of preantral follicles in mice

Wang

Sun

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…Moreover, the growth promotion mechanisms were significantly associated with the reduction of ROS levels during follicle development. Considering the growth of preantral follicles during the long-term culture process in vitro, the analyses of RT-PCR and hormone levels were conducted after 14 days of follicle culture [45,46]. After BAPT treatment, the ROS production-related genes as SOD2, Catalase and GPx, which regulated the cellular antioxidant defense system, were significantly up-regulated with the increasing dose of BAPT, indicating that BAPT treatment significantly inhibited ROS production process of isolated follicles cultured in vitro.…”

Section: Discussionmentioning

confidence: 99%

Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles

Liu

Ren

et al. 2020

J Ovarian Res

View full text Add to dashboard Cite

The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research.

show abstract

Oviductus Ranae Promotes Follicle Growth Through the PI3K/AKT Signaling Pathway In Vivo and In Vitro in Rat

Zhao,

Wang,

Sun

et al. 2024

Food Science & Nutrition

View full text Add to dashboard Cite

The development status of follicles determines the menstrual cycle and estrogen levels, which is crucial to women's health. Oviductus Ranae is a natural product for both medicine and food, which has “estrogenic effect”. However, few studies have systematically elaborated its mechanism of action. Hence, we hypothesize the “estrogen‐like effects” of OR may stem from its positive influence on the growth and development of growing follicles in the ovaries. In this study, the effect of Oviductus Ranae (OR) on the growth and development of rat follicles and follicles cultured in vitro was investigated. The content of estrogen in rat serum and follicular culture medium in vitro was determined by radioimmunoassay, and the levels of PI3K‐Akt signal pathway and FSHR expression in rat ovary and cultured follicles were detected by RT‐PCR and Western Blot. The follicles at different developmental stages in rat ovaries were analyzed by H&E and TUNEL staining. Follicles cultured in a medium containing OR displayed a significant increase in diameter, and the E2 content in the medium was significantly increased. Moreover, Rats treated with OR showed significant increases in estradiol and progesterone levels. The number of antral follicles in the ovaries displayed a significant increase, while the percentage of atretic antral follicles and the total atretic follicles showed significant decreases. The relative expression levels of PI3K, Akt, and FSHR were significantly increased, while significantly decreased in that of PTEN In Vivo and In Vitro. OR may promote follicle growth through PI3K/ Akt pathway, exhibiting “estrogen‐like effects”.

show abstract

Activin effects on follicular growth in <i>in vitro</i> preantral follicle culture

Cited by 11 publications

References 42 publications

The optimized research of the in vitro culture of preantral follicles in mice

The optimized research of the in vitro culture of preantral follicles in mice

Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles

Oviductus Ranae Promotes Follicle Growth Through the PI3K/AKT Signaling Pathway In Vivo and In Vitro in Rat

Contact Info

Product

Resources

About