Activities of Antioxidant and Detoxifying Enzymes in Rats after Lead Exposure

Abstract

Abstractalghazal m. a., V. lenártová, K. holovská, a. sobeková, m. falis, j. legáth: activities of antioxidant and Detoxifying enzymes in rats after lead exposure. acta Vet. brno 2008, 77: 347-354. the studies were undertaken to investigate the activity response of the antioxidant enzyme superoxide dismutase (soD) and detoxifying enzyme glutathione-s-transferase (gst) of rats exposed to lead. enzyme activities were determined in the liver, kidneys and heart of male and female rats that received 100 mg and 1000 mg of lead acetate per litre, respectively, in their drinking water for 18 weeks. statistical analyses indicated differences related to the organs and to the sex of animals. administration of lead evoked an increase of the soD activity in the liver and kidneys both in male and female rats but only in the heart of female rats. gst activity decreased in the liver and heart of male rats, while this activity increased in the liver and heart of female rats. in kidneys, the lower lead dose resulted in a decrease of the gst activity in both groups but the higher dose evoked an increase of activity only in male rats. thiobarbituric acid reactive substances (TBARS), an indicator of oxidative stress, significantly increased in rats that were given the high lead dose, in the kidneys of male rats and in the heart of female rats. most probably, the observed changes could be a compensatory response to different lead accumulation in the male and female organs and also the possible distinct mechanisms in ros elimination.

Ginger extract modulates Pb-induced hepatic oxidative stress and expression of antioxidant gene transcripts in rat liver

“…Alghazal et al (2008) reported an increase in GST following exposure to inorganic Pb. Consequently, increased levels of GST and GSH, the substrate for GST, may result from Pb-generated injuries.…”

Cardio protective effects of Nigella sativa oil on lead induced cardio toxicity: Anti inflammatory and antioxidant mechanism

“…There is an association between CVD, inflammation and oxidative stress (Roshan et al, 2011). Lead (Pb) is a non-essential toxic heavy metal widely distributed in the environment; various physiological, biochemical and behavioral dysfunctions are induced by chronic exposure to low levels of pb (Ahamed and Siddiqui, 2007;Alghazal et al, 2008).…”

“…of prooxidant and antioxidant balance by generation of reactive oxygen species (ROS) (Alghazal et al, 2008;Roshan et al, 2012). Adipokines such as apelin, Creactive protein (CRP), tumor necrosis factor TNF-α and E-selectin represent a family of proteins released by adipocytes that affect various biological processes including metabolism, satiety, inflammation and cardiovascular function (Gaeini et al, 2008).…”

In vivoantioxidant potential of ascorbic acid against lead and/or cypermethrin-induced oxidative tissue damage

“…These findings are in agreement with the results obtained by Valverde, Trejo, and Rojas (2001) and Marchlewicz et al (2007), suggesting induction of Pb cytotoxicity by indirect mechanisms. Evidence of a dose response relationship between Pb exposure and LPO levels in cardiac tissue was previously reported (Alghazal et al 2008).…”

“…The elevated lipid hydroperoxides in liver, kidney and spleen observed in the current study in Pb and/or CPMtreated group are indicative of membrane damage due to formation of free radicals or through exhaustion of antioxidants, leading to oxidative stress. Lead is known to induce oxidative damage in various tissues (Jackie, Haleagrahara, and Chakravarthi 2011;Hamadouche et al 2009;Alghazal et al 2008;Abdel-Moneim, Dkhil, and Al-Quraishy 2011). These findings are in agreement with the results obtained by Valverde, Trejo, and Rojas (2001) and Marchlewicz et al (2007), suggesting induction of Pb cytotoxicity by indirect mechanisms.…”