Activities of DNA Turnover and Free Radical Metabolizing Enzymes in Cancerous Human Prostate Tissue

Biri, Hasan; Öztürk, Hakan; Kaçmaz, Murat; Karaca, K; Tokuçoğlu, Haluk; Durak, İlker

doi:10.3109/07357909909032872

Cited by 42 publications

(22 citation statements)

References 36 publications

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“…Observations similar to our findings have been reported in human colorectal cancer [29], lung cancer [30], colorectal adenocarcinomas [31], human malignant tumors of the kidney [32] and prostate cancer [33]. However, this does not agree with the results obtained on tissue MDA levels in OSCC, as studied by Balasenthil et al [34 ]and Saroja et al [35].…”

Section: Discussioncontrasting

confidence: 54%

Enhanced Nitrosative and Oxidative Stress with Decreased Total Antioxidant Capacity in Patients with Oral Precancer and Oral Squamous Cell Carcinoma

Korde¹,

Basak

Chaudhary³

et al. 2011

Oncology

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Objective: Interaction between reactive oxygen species (ROS) and reactive nitrogen species (RNS) is a crucial determinant in the etiology of oral squamous cell carcinoma (OSCC), and antioxidants protect against cellular and molecular damage caused by these. This study aims to evaluate ROS and RNS, together with the total antioxidant capacity (TAC), in serum and tissues of OSCC patients. Method: Total nitric oxide (TNO), malondialdehyde (MDA), and TAC were evaluated using a UV visible spectrophotometer in the serum of 30 controls, 30 precancer and 30 OSCC patients and in tissues of 10 controls and 30 OSCC patients. Results: MDA and TNO were significantly elevated with concomitant depletion of TAC in serum and tissues of OSCC patients when compared to controls. A significant positive correlation (p = 0.01), at different levels between TNO and MDA in OSCC patients, was found, which increased with the grade of OSCC. Conclusion: An increase in the levels of TNO, NO-mediated lipid peroxidation, resulting in an increase in MDA levels, the positive TNO-MDA correlation with a deranged antioxidant defense system as demonstrated by significantly low levels of TAC; when all these facts are taken together, we can conjecture that oxidative DNA damage, a vital phenomenon for carcinogenesis, occurs due to the interplay of ROS, RNS and TAC.

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Section: Discussioncontrasting

confidence: 54%

Enhanced Nitrosative and Oxidative Stress with Decreased Total Antioxidant Capacity in Patients with Oral Precancer and Oral Squamous Cell Carcinoma

Korde¹,

Basak

Chaudhary³

et al. 2011

Oncology

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“…the higher the rate of lipid peroxidation in the cells the lower the rate of cell division. [26] Our findings were in agreement with the reports of Biri et al; [27] Yilmaz et al, [28] who have reported increased TBARS concentrations suggesting oxidative stress and accelerate peroxidative reactions in the cancerous prostate tissues, even though antioxidant defense mechanisms were activated. However, DogruAbbasoglu et al [29] have found no significant change in lipid peroxidation or antioxidant system parameters in the plasma of patients with BPH and prostate cancer.…”

Section: Applicationssupporting

confidence: 93%

Rapid and Simple Determination of Plasma and Erythrocyte MDA Levels in Prostate Cancer Patients by a Validated HPLC Method

Arsova‐Sarafinovska

Aydın

Sayal

et al. 2007

Journal of Liquid Chromatography & Related Technologies

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Abstract:We undertook the present study to develop a validated HPLC method for the determination of malondialdehyde (MDA) levels and to use this method for determination of MDA levels in patients with prostate cancer and benign prostatic hyperplasia.MDA levels were estimated in the erythrocyte and plasma sample of the 25 non-metastatic prostate cancer patients, 36 benign prostatic hyperplasia (BPH) patients We report a very rapid and simple isocratic reversed-phase HPLC separation of MDA in normal human plasma and erythrocytes without previous purification of the MDA-TBA complex. All validation parameters were performed in our methods. Using this methods we have found elevated MDA in the plasma and erythrocyte of the prostate cancer group as compared to controls and BPH group.We have improved and validated an analytical HPLC method for determination of MDA in plasma and erythrocyte, which is simple to perform and having high sensitivity, specificity and substantial improvement in column life. This method has been successfully applied to determination of MDA levels in prostate cancer patients and offers an oportunity to further characterize the role of oxidative injury in the pathogenesis of this disease specifically.

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“…The specific activity of ADA in cancerous human prostate has been reported to be similar or slightly less than in normal prostatic tissue (36,37). However, ADA levels in benign prostatic hypertrophy (BPH) tissue were significantly higher than in normal prostate (36).…”

Section: Discussionmentioning

confidence: 92%

Cell Surface Adenosine Deaminase Binds and Stimulates Plasminogen Activation on 1-LN Human Prostate Cancer Cells

Gonzalez-Gronow

Hershfield

Arredondo-Vega

et al. 2004

Journal of Biological Chemistry

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Adenosine deaminase (ADA) is expressed intracellularly by all cells, but in some tissues, it is also associated with the cell surface multifunctional glycoprotein CD26/ dipeptidyl peptidase IV. By modulating extracellular adenosine, this "ecto-ADA" may regulate adenosine receptor signaling implicated in various cellular functions. CD26 is expressed on the surface of human prostate cancer 1-LN cells acting as a receptor for plasminogen (Pg). Since ADA and Pg bind to CD26 at distinct but nearby sites, we investigated a possible interaction between these two proteins on the surface of 1-LN cells. Human ADA binds to CD26 on the surface 1-LN cells and immobilized CD26 isolated from the same cells with similar affinity. In both cases, ADA binding is diminished by mutation of ADA residues known to interact with CD26. ADA was also found to bind Pg 2 in the absence of CD26 via the Pg kringle 4 (K4) domain. In the presence of 1-LN cells or immobilized CD26, exogenous ADA enhances conversion of Pg 2 to plasmin by 1-LN endogenous urinary plasminogen activator (u-PA), as well as by added tissue Pg Activator (t-PA), suggesting that ADA and Pg bind simultaneously to CD26 in a ternary complex that stimulates the Pg activation by its physiologic activators. Consistent with this, in melanoma A375 cells that bind Pg, but do not express CD26, the rate of Pg activation was not affected by ADA. Thus, ADA may be a factor regulating events in prostate cancer cells that occur when Pg binds to the cell surface and is activated.

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Activities of DNA Turnover and Free Radical Metabolizing Enzymes in Cancerous Human Prostate Tissue

Cited by 42 publications

References 36 publications

Enhanced Nitrosative and Oxidative Stress with Decreased Total Antioxidant Capacity in Patients with Oral Precancer and Oral Squamous Cell Carcinoma

Enhanced Nitrosative and Oxidative Stress with Decreased Total Antioxidant Capacity in Patients with Oral Precancer and Oral Squamous Cell Carcinoma

Rapid and Simple Determination of Plasma and Erythrocyte MDA Levels in Prostate Cancer Patients by a Validated HPLC Method

Cell Surface Adenosine Deaminase Binds and Stimulates Plasminogen Activation on 1-LN Human Prostate Cancer Cells

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